BTE003 Structure elucidation methods in bioactive molecules

Information

Course Goal

The objective of this course, it is aimed to teach the methods used in elucidating the structure of bioactive molecules and the techniques applied in these methods together with the strategies for elucidating the structure of bioactive molecules.

Course Content

Molecular spectroscopy, Electromagnetic Spectrum, UV-Visible Spectroscopy, UV-Absorption bands of some pharmaceutical and chromophore groups, Applications of IR-spectroscopy in bioactive compounds, Interpretation of IR-Spectrums, IR-Spectroscopy in the Identification of functional groups in drug or bioactive molecules, Raman spectroscopy, Nuclear Magnetic Resonance (NMR) Spectroscopy, Chemical Shift and Spin-Spin Interactions, Multiplicity of the signal, 1 H-NMR 13 C-NMR, 2 D-COSY, DEPT, HETCOR, NOESY and nOe differences techniques. Interpretation of NMR Spectrums, Use of NMR spectroscopy for structure elucidation of bioactive and drug molecules, MS Spectrometry, Spectrums evaluation and use of structural determination in drug and bioactive molecules. Single crystal X-ray spectroscopy and its use in structure determination, Use of Combined Spectral Analysis Methods in the analysis of drugs, bioorganic and bioactive compounds. Identification of stereochemistry in bioactive molecules. Determination of chirality in bioactive molecules.

Course Precondition

none

Resources

Lecture notes

Notes

Horst Friebolin Transl. by Jack Becconsall Basic One-and-Two-dimensional NMR spectroscopy, 1993 Pavia DL., Lampman GM., Kriz GS. Introduction to Spectroscopy , Saunders College Publishing, 1979 lremore, I.R., Evaluation of Instrumentation for UV-Visible Spectrophotometry, J. Chem. Educ., 63 : A, 216, 1986 Nakanishi, K., Infrared Absorption Spectroscopy, 2nd ed., San Fransisco, Holden Day, 1997 Kar, A, Pharmaceutical Drug Analysis, New Age Int. Publishers, 2005 Williams H. D., Fleming I. Spectroscopic methods in organic chemistry. Fourth Edt. Revised, McGraw-Hill Book Company, England, 1989 Balcı M. Nükleer manyetik rezonans Spektroskopisi. ODTÜ Geliştirme Vakfı Yayıncılık ve İletişim A.Ş. Birinci Baskı, Ankara, 2000 De Hoffmann E., Stoobant V. Mass Spectroscopy. Newyork, John wiley Sons, Second Ed. 2001 Rene E. Van Grieken, Andrzej A. Markowicz, Handbook of X-ray spectrometry: methods and techniques, 1993 Manz, Pamme, N., Lossfidis, Bioanalytical Chemistry, Imperial colege Press, 2003 Erdik, E; Organik Kimyada Spektroskopik Yöntemler, 3. Baskı, Gazi Kitapevi, 2005

Course Learning Outcomes

Relation with Program Learning Outcome

Week Plan

Student Workload - ECTS