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Information
| Unit | FACULTY OF SCIENCE AND LETTERS |
| BIOLOGY PR. | |
| Code | BL317 |
| Name | Moleculer Biology |
| Term | 2017-2018 Academic Year |
| Semester | 5. Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | C Compulsory |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BURHAN ARIKAN |
| Course Instructor |
Prof. Dr. BURHAN ARIKAN
(Güz)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
To teach nucleic acid in different microorganism, mobile genetic materials and their isolation, gene cloning, recombinant DNA methods, PCR and their applications
Course Content
Introductions to Molecular Biology, Plasmids and Bacteriophages, Isolation of Plasmid and Bacteriophages nucleic acids, properties and impotance of transposane and transposable structural, DNA isolation of animal and plant cells, The mechanisms of natural genetic material of microorganisms and plant cells, transformation (CaCL2, RbCL2, Protoplast and Liposome) electroporation, electrofusion, biolistic transformation, gene cloning, ploymerase chain reaction, hybridization (southern, northern, ın situ and dot blot), cloning vectors, recombinant DNA production and transfer to recipient cell, molecular diagnostic methods (DNA fingerprint, RFLP, 16S ribosomal RNA genes, RAPD)
Course Precondition
BL 317 Moloküler Biyoloji
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explains gene cloning and recombinant DNA techniques and their applications |
| LO02 | Explain plasmids and bacteriophages, their properties, plasmid DNA and bacteriophage nucleic acids. |
| LO03 | List the genetic material transfers and methods that occur in microorganisms in the natural environment. |
| LO04 | Explains cloning vectors and their properties. |
| LO05 | Explains conjugation and biolistic transformation methods and their applications. |
| LO06 | Categorize gene cloning and cloning methods |
| LO07 | Discuss the production of recombinant DNA and its transfer to the recipient organism |
| LO08 | Explains the stages of gene cloning and cloning methods. |
| LO09 | Explains PCR (Polymerase chain reaction and its application areas) |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Explain the basic concepts and principles in biology | |
| PLO02 | Bilgi - Kuramsal, Olgusal | The knowledge gained in the field of biology combine with information from other sciences. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Identifies problems in the field of biology and apply methods for their solution | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Describes the method of producing scientific knowledge in the field of biology | |
| PLO05 | Bilgi - Kuramsal, Olgusal | They will receive information on the biology area, in order to classify it uses and purposes. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | They use advanced theoretical and practical knowledge that obtain in biology field. | |
| PLO07 | Bilgi - Kuramsal, Olgusal | They are Field-related issues can inform the relevant persons and institutions, They share ideas and problems relating to suggestions as the written, oral and Visual to expert or transfer to non-experts. | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | They can take responsibility as individuals and team members to solve unpredictable problems encountered in applications related to the field. | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | they can take an active role as a member of the execution team the planning of projects related to the field. | 3 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | They know that life-long learning process and act accordingly. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They will increase the knowledge and experience outside major issues with specific elective courses in different fields of biology. | 2 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They speak English enough to communicate with colleagues to understand the basic concepts of biology. | |
| PLO13 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They both with colleagues, as well as areas with non-person establishes ethical uses of information and communication technology. In addition to professional development, in line with the interests and abilities of scientific, social, constantly improves itself by identifying learning needs in cultural and artistic fields. | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | They assesses developments in biology versatile using scientific methods and techniques | |
| PLO15 | Yetkinlikler - Öğrenme Yetkinliği | They know the importance of personal development and enforces the need. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introductions to Molecular Biology | Study in literature | |
| 2 | Plasmids and Bacteriophages, Isolation of Plasmid and Bacteriophages nucleic acids | Study in literature | |
| 3 | properties and impotance of transposane and transposable structural | Study in literature | |
| 4 | DNA isolation of animal and plant cells | Study in literature | |
| 5 | The mechanisms of natural genetic material of microorganisms and plant cells | Study in literature | |
| 6 | Conjugation, electroporation, electrofusion, biyolistic transformation | Study in literature | |
| 7 | Transformation CaCL2, RbCL2, Protoplast and Liposome | ||
| 8 | Exam | Study in literature | |
| 9 | gene cloning and cloning techniques | Study in literature | |
| 10 | PCR | Study in literature | |
| 11 | Hibridisation (Souther, northern, In situ, Dot bloth) | Study in literature | |
| 12 | cloning vectors | Study in literature | |
| 13 | recombinant DNA production and transfer to recipient cell | Study in literature | |
| 14 | Isolation of recombinant organisms | Study in literature | |
| 15 | Hibridoma and cell fusion techniques | Study in literature | |
| 16 | Final exam | ||
| 17 | Final exam |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 40 |
| General Assessment | ||
| Midterm / Year Total | 100 | 40 |
| 1. Final Exam | - | 60 |
| Grand Total | - | 100 |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 3 | 3 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||