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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| HISTOLOGY AND EMBRYOLOGY (MEDICINE) (MASTER) (WITH THESIS) | |
| Code | HİS521 |
| Name | Histological Techniques I (Z) |
| Term | 2018-2019 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. YURDUN KUYUCU |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Aim of this course is learning and applying tissue preparation techniques for light microscopic and electron microscopic evaluation of organs and tissues .
Course Content
Microscope types, light and electron microscopic tissue preparation and staining protocols and histochemical techniques
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows how to use a light microscobe. |
| LO02 | Prepares solutions for light and electron microscopic tissue preperation. |
| LO03 | Applies fixation techniques |
| LO04 | Applies dehydration and clearing of a tissue section. |
| LO05 | Be able to embed the tissue sample appropriately. |
| LO06 | Applies sectioning from a microtome and ultramicrotome. |
| LO07 | Applies staining methods. |
| LO08 | Knows the basics of histochemical staining methods. |
| LO09 | Knows the histochemical staining methods for nucleus and cytoplasmic organelles, connective tissue and enzymes. |
| LO10 | Takes photos of the specimen on light microscope. |
| LO11 | Offers solutions to problems at any technique step of the staining and preparation method. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Tell the basic histological features of cells and tissues. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Can establish connection betwwen the structure and function of the cells. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Have knowledge about the histological properties of organ systems. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Have knowledge about embryo development, development disorders and mechanisms. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Define light and electron microscopic tissue preparation and examination methods. | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has knowledge about general principles of cell culture, histochemistry and immunohistochemistry. | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Prepare tissues for light and electron microscopic analysis. | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Apply basic histochemical staining methods. | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Can distinguish tissue and organ systems at light microscopic level. | 5 |
| PLO10 | Bilgi - Kuramsal, Olgusal | He / she carries out his / her study as an individual or a team member. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Do the tasks given within the scope of research projects. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Develop a positive attitude and behavior related to lifelong learning. | |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Identifies learning needs and resources, and guides them by using resources effectively. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Evaluate the knowledge and skills at the graduate level with a critical approach. | |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Use information and communication technologies at the level required by the field. | |
| PLO16 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Communicate effectively. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transfer their ideas and solutions by quantitative and qualitative datas by supporting the current developments and their studies orally and visiually | |
| PLO18 | Yetkinlikler - Öğrenme Yetkinliği | Knows the importance of ethical principles in conducting research studies, evaluating and sharing the results. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General information about the methods of histological examination | Self-study, reading recommended references | |
| 2 | Microscope types and usage areas | Self-study, reading recommended references | |
| 3 | Light microscopic tissue preparation methods | Self-study, reading recommended references | |
| 4 | Light microscopic tissue staining methods | Self-study, reading recommended references | |
| 5 | Preparation methods for evaluation of hard tissues | Self-study, reading recommended references | |
| 6 | Special stains for determining the hematopoietic and lenforeticular tissue elements | Self-study, reading recommended references | |
| 7 | Basic principles of the histochemistry | Self-study, reading recommended references | |
| 8 | Mid-Term Exam | Self-study, reading recommended references | |
| 9 | Histochemical techniques used for connective tissue | Self-study, reading recommended references | |
| 10 | Histochemical techniques used for nerve tissue | Self-study, reading recommended references | |
| 11 | Histochemical techniques used in demonstration of nuclear and cytoplasmic elements | Self-study, reading recommended references | |
| 12 | Histochemical techniques used for enzymes, carbohydrates and lipids | Self-study, reading recommended references | |
| 13 | Electron microscopic tissue preparation techniques | Self-study, reading recommended references | |
| 14 | Solution and stains used in electron microscopic tissue preparation | Self-study, reading recommended references | |
| 15 | Artefacts | Self-study, reading recommended references | |
| 16 | Term Exams | Self-study, reading recommended references | |
| 17 | Term Exams |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 20 | 20 |
| Final Exam | 1 | 21 | 21 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||