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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| Code | MİK612 |
| Name | Molecular Identification Methods of Viral Infections |
| Term | 2026-2027 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. FÜGEN YARKIN |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To teach students current molecular techniques used in the diagnosis of viral infections and to provide skills in interpreting results and applying them in clinical laboratory practice.
Course Content
Molecular methods used in the diagnosis of viral infections, including nucleic acid amplification techniques (PCR, RT-PCR), real-time PCR, sequencing methods, genotyping, and rapid molecular tests are covered. Processing of clinical samples, interpretation of results, and introduction to laboratory applications are also included.
Course Precondition
Basic knowledge of Microbiology and Virology is recommended.
Resources
Murray Medical Microbiology Fields Virology Jawetz, Melnick & Adelberg Medical Microbiology Course lecture notes on molecular microbiology and virology
Notes
Recent peer-reviewed articles WHO molecular diagnostic guidelines World Health Organization Clinical virology and molecular diagnostics textbooks
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Defines molecular methods used in the diagnosis of viral infections. |
| LO02 | Explains the principles of PCR, RT-PCR and real-time PCR techniques. |
| LO03 | Explains the laboratory detection processes of viral nucleic acids. |
| LO04 | Compares the advantages and limitations of molecular diagnostic methods. |
| LO05 | Describes steps of viral DNA/RNA extraction from clinical samples. |
| LO06 | Interprets the basic principles of sequencing and genotyping methods. |
| LO07 | Evaluates molecular diagnostic results in a clinical context. |
| LO08 | Explains the applications of rapid molecular tests in viral infections. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Beceriler - Bilişsel, Uygulamalı | Having acquired technical skills through laboratory studies, being able to access information, conduct research, plan experiments, solve problems, interpret experimental results, make presentations, develop strategies on microbiology-related issues | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Having received a quality microbiology education at a standard accepted in international platforms, having broadened scientific horizons and being preferred in many sectors related to microbiology, being competent in microbiology | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Isolates infectious agents | 2 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Performs the steps of identifying infectious agents | 2 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Knowing and applying laboratory methods used in general bacteriology, virology, parasitology, mycology and immunology at theoretical and practical levels | 2 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Being aware of ethical values and problems in the field of microbiology | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Ability to make critical evaluations on published research in the field of microbiology | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Gaining the competence to present a research conducted in the field of microbiology in written, oral and visual form and prepare it for publication | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Having acquired research and career ethics | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Having deepened knowledge on specific subjects studied (thesis study) |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to molecular diagnostics and basic concepts | Reading introduction to molecular diagnostic methods | Öğretim Yöntemleri: Anlatım |
| 2 | Nucleic acid structure and viral genomes | Review of DNA and RNA structures | Öğretim Yöntemleri: Anlatım |
| 3 | Principle and basic applications of PCR | Reading basic principles of PCR | Öğretim Yöntemleri: Anlatım |
| 4 | RT-PCR and viral RNA detection | Knowledge of RNA viruses and reverse transcription | Öğretim Yöntemleri: Anlatım |
| 5 | Real-time PCR (qPCR) | Review of qPCR basics | Öğretim Yöntemleri: Anlatım |
| 6 | Sample collection and nucleic acid extraction | Review of laboratory sample types | Öğretim Yöntemleri: Anlatım |
| 7 | Viral DNA/RNA extraction methods | Reading extraction steps | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | PCR contamination and quality control | Laboratory quality control concepts | Öğretim Yöntemleri: Anlatım |
| 10 | Primer and probe design in molecular diagnostics | Basic knowledge of PCR primers | Öğretim Yöntemleri: Anlatım |
| 11 | Sequencing methods (Sanger and NGS) | Basic principles of DNA sequencing | Öğretim Yöntemleri: Anlatım |
| 12 | Genotyping and variant analysis | Review of viral mutation concepts | Öğretim Yöntemleri: Anlatım |
| 13 | Rapid molecular tests and POCT | Reading rapid diagnostic systems | Öğretim Yöntemleri: Anlatım |
| 14 | Interpretation of molecular diagnostic results | Review of PCR result reports | Öğretim Yöntemleri: Anlatım |
| 15 | next-generation molecular diagnostic methods (CRISPR/Cas, NGS, isothermal tests) | General review of all next-generation molecular diagnostic methods (CRISPR/Cas, NGS, isothermal amplification) | Öğretim Yöntemleri: Anlatım, Gösteri |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 6 | 6 |
| Total Workload (Hour) | 96 | ||
| Total Workload / 25 (h) | 3,84 | ||
| ECTS | 4 ECTS | ||