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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (MASTER) (WITH THESIS) (INTERDISCIPLINARY) | |
| Code | BT004 |
| Name | Introduction to Basic Molecular Biology Techniques |
| Term | 2018-2019 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MEHMET BERTAN YILMAZ |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To learn genome analysis methods,polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, PCR and QF PCR results analysis and interpretation, PCR types Time PCR methods and applications, Blotting techniques, Application areas (PCR RFLP, Sequence analysis, Gene cloning).
Course Content
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, Analysis and interpretation of PCR and QF PCR results.PCR types and application areas (mutation screening, PCR RFLP, Sequence analysis, Gene cloning), Real Time PCR methods and applications, Blotting techniques
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | To have knowledge of genome analysis methods |
| LO02 | To have knowledge of Polymerase chain reaction (PCR) |
| LO03 | To have knowledge of agarose and polyacrylamide gel electrophoresis |
| LO04 | To have knowledge of Real Time PCR |
| LO05 | To have knowledge of blotting techniques |
| LO06 | To have knowledge of application areas ( Mutation screening, PCR,RFLP, Sequence analysis, Gene cloning, |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | - | Gains the ability to develop and deepen the knowledge in the field of biotechnology | |
| PLO03 | - | Identifies the interdisciplinary interaction of the field | |
| PLO04 | - | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | |
| PLO05 | - | Solve problems encountered in the field by using research methods. | |
| PLO06 | - | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO07 | - | Solve problems encountered in the field by using research methods. | |
| PLO08 | - | It supervises and teaches the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Genome analysis methods | Literature review and reading | |
| 2 | Polymerase chain reaction (PCR) mechamisms | Literature review and reading | |
| 3 | Polymerase chain reaction (PCR) optimization | Literature review and reading | |
| 4 | DNA isolation | Literature review and reading | |
| 5 | Agarose and polyacrylamide gel electrophoresis | Literature review and reading | |
| 6 | Preparation of solutions and gels and staining of gels | Literature review and reading | |
| 7 | QF PCR method and application | Literature review and reading | |
| 8 | Mid-Term Exam | question preparation | |
| 9 | PCR types | Literature review and reading | |
| 10 | PCR and QF PCR results analysis and interpretation | Literature review and reading | |
| 11 | Real Tıme PCR methods and applications | Literature review and reading | |
| 12 | Blotting techniques | Literature review and reading | |
| 13 | Application areas (mutation screening, PCR RFLP) | Literature review and reading | |
| 14 | Application fields (Sequence analysis, Gene cloning) | Literature review and reading | |
| 15 | Review of topics and brainstorming | question preparation | |
| 16 | Term Exams | question preparation | |
| 17 | Term Exams | question preparation |