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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (MASTER) (WITH THESIS) (INTERDISCIPLINARY) | |
| Code | BT014 |
| Name | Polymerase Chain Reaction (PCR), Optimisation and Screening of the Results |
| Term | 2018-2019 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. ARMAĞAN ERDEM ÜTÜK |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of the course is to enable the graduates to have knowledge about Polymerase Chain Reaction, to be able to apply the test and to solve the problems they may encounter during the applications.
Course Content
The components of this course consist of Polymerase Chain Reaction, it's standardisation and the evaluation of the test results.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Students will learn the molecular structure of the cell |
| LO02 | Students will learn DNA ekstraction from the tissues |
| LO03 | Students will have a knowledge about PCR and PCR based molecular techniques |
| LO04 | Students will solve the problems while they are using PCR and optimise the test |
| LO05 | Student can perform electrophoresis and imaging processing of PCR products |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | - | Gains the ability to develop and deepen the knowledge in the field of biotechnology | |
| PLO03 | - | Identifies the interdisciplinary interaction of the field | |
| PLO04 | - | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | |
| PLO05 | - | Solve problems encountered in the field by using research methods. | |
| PLO06 | - | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO07 | - | Solve problems encountered in the field by using research methods. | |
| PLO08 | - | It supervises and teaches the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Structure of the cell | Lecture notes,slide projection | |
| 2 | The structure and replication of of DNA | Lecture notes,slide projection | |
| 3 | Transcription and translation | Lecture notes,slide projection | |
| 4 | Gen, Genom and Regulation of DNA | Lecture notes,slide projection | |
| 5 | Mutations (Genom, Gen and DNA mutations) | Lecture notes,slide projection | |
| 6 | DNA extraction methods | Lecture notes,slide projection | |
| 7 | Introduction to Polymerase Chain Reaction (PCR) | Lecture notes,slide projection | |
| 8 | Mid-Term Exam | Oral exam | |
| 9 | Modifications of PCR (Semi-Nested PCR, Nested PCR, Multiplex-PCR, RAPD-PCR, Real Time-PCR) | Lecture notes,slide projection | |
| 10 | Optimisation in PCR ( enzyme, buffer, magnesium chloride, potassium chloride, dNTP mix, primers) | Lecture notes,slide projection | |
| 11 | Optimisation in PCR (Optimisation of PCR cycles) | Lecture notes,slide projection | |
| 12 | Preparation of gels (agarose, PAGE) that used in the electrophoresis of the amplicons | Lecture notes,slide projection | |
| 13 | Electrophoresis, staining of PCR gels and screanin of the results | Lecture notes,slide projection | |
| 14 | Optimisation in the electrophoresis, jel preparation and screening | Lecture notes,slide projection | |
| 15 | Problems in PCR and solution offers | Lecture notes,slide projection | |
| 16 | Term Exams | Oral exam | |
| 17 | Term Exams |