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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| BIOPHYSICS (MEDICINE) (PhD) | |
| Code | BFZ612 |
| Name | Microscopy and Fluorescent Imaging of the Cell |
| Term | 2018-2019 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 7 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. FİGEN ÇİÇEK |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Learning basic principles of microscopes and decide and choose a proper microscope for a scientific project and use a microscope. Learning fluorescence imaging techniques in the cell and ability to do fluorescence imaging .
Course Content
Basic optic principles, types of microscopes and their working principles. Fluorescent principles and its use in cellular imaging.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns basics of light and optics. |
| LO02 | learns the basic principles of image formation in the Microscope |
| LO03 | Learns the selection criteria of the appropriate microscope for the experiments. |
| LO04 | Learns to use a microscope in the laboratory and image data analysis. |
| LO05 | Learns fluorescence with its physics principles |
| LO06 | Learns different fluorescence imaging techniques |
| LO07 | . Learns to decide florophore, type of the florescent system, wavelength and filters according to the experiment. |
| LO08 | Learns image data analysis. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To learn the basic information about the field | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To be able to establish experimental work in laboratory | |
| PLO03 | Bilgi - Kuramsal, Olgusal | To prepare a project proposal by itself | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To plan an experimental research | |
| PLO05 | Bilgi - Kuramsal, Olgusal | To make a report as a result of the research | 1 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To be able to interpret research findings | |
| PLO07 | Bilgi - Kuramsal, Olgusal | Having analytical thinking mechanism related to the field Accessing reliable information Creating and writing a research project Study and research based on ethic values | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Ability to transfer the theoretical knowledge to the audience in a systematic way | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To have scientific thought systematic about the profession | |
| PLO10 | Bilgi - Kuramsal, Olgusal | Kendi başına bir proje önerisi hazırlayabilmek | 1 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Light and alectromagnetic spectrum | Reading the related chapter from the textbooks and notes | |
| 2 | Basic optic principles | Reading the related chapter from the textbooks and notes | |
| 3 | Interference, diffraction and resolving power, polarization | Reading the related chapter from the textbooks and notes | |
| 4 | Optical components of the simple microscopes. Magnification and numerical aperture | Reading the related chapter from the textbooks and notes | |
| 5 | Lighting techniques in the microscopes (1); bright field, dark field, phase contrast, cross polarized light | Reading the related chapter from the textbooks and notes | |
| 6 | Lighting techniques in the microscopes (2); Microspectroscopy (UV and UV-vis) epifluorescence microscopes, confocals | Reading the related chapter from the textbooks and notes | |
| 7 | Other microscope types; Atomic Force, X Ray microscopy, electron microscopy | Reading the related chapter from the textbooks and notes | |
| 8 | Mid-Term Exam | ||
| 9 | Basics of the Fluorescence | Reading the related chapter from the textbooks and notes | |
| 10 | Fluorescence probes and their cellular usages | Reading the related chapter from the textbooks and notes | |
| 11 | Fluorescent measurement systems and their working principles; Fluorimeter, Camera and confocal systems | Reading the related chapter from the textbooks and notes | |
| 12 | Ion imaging in the cell and calibration | Reading the related chapter from the textbooks and notes | |
| 13 | Ion imaging in the cell and calibration | Reading the related chapter from the textbooks and notes | |
| 14 | Measurement of dynamic ion concentrations in the cell | Reading the related chapter from the textbooks and notes | |
| 15 | Fluorescent meaurement of DNA and immunofluorescent techniques | Reading the related chapter from the textbooks and notes | |
| 16 | Term Exams | ||
| 17 | Term Exams |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 9 | 9 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 173 | ||
| Total Workload / 25 (h) | 6,92 | ||
| ECTS | 7 ECTS | ||