TÜRKÇE
ENGLISH
Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| HORTICULTURAL CROPS (MASTER) (WITH THESIS) | |
| Code | BB595 |
| Name | Laboratory Technics of Plant Biotechnology |
| Term | 2019-2020 Academic Year |
| Term | Fall |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of this course is to examine the main techniques applied in plant biotechnology laboratory.
Course Content
The contents of this course include the definition and application of plant biotechnology, solution preparation, microscope, centrifuge, use of PHmeter in biotechnology, plant tissue cultures, the preparation of liquid-solid LB medium, creation of a single colony of bacteria, Agrobacterium tumefaciens-mediated gene transfer, DNA isolation and quantification, PCR applications, agarose gel electrophoresis, polyacrylamide gel electrophoresis, DNA sequence analysis.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications |
| LO02 | Understanding the horticulture and the interaction between the related disciplines |
| LO03 | Solving problems by research methods and establishing cause-effect relationship |
| LO04 | Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. |
| LO05 | Carrying out a study individually that requires professional knowledge in Horticulture |
| LO06 | Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results |
| LO07 | Leading in the situations when solving problems related to horticulture is necessary |
| LO08 | Mid-Term Exam |
| LO09 | Entering database related to horticulture, utilizing those sources and self-updating |
| LO10 | Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process |
| LO11 | Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments |
| LO12 | Using software related to horticulture sufficiently and using information and communication technologies in advanced level |
| LO13 | Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values |
| LO14 | Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating |
| LO15 | Evaluation of the basic techniques used in plant biotechnology |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies | 0 |
| PLO02 | - | To be able to understand the interaction between horticultural field and related disciplines | 0 |
| PLO03 | - | To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative | 0 |
| PLO04 | - | Solving problems by using research methods and solving cause and effect relationships | 0 |
| PLO05 | - | To be able to conduct a study requiring expertise on horticultural plants independently | 0 |
| PLO06 | - | To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained | 0 |
| PLO07 | - | To lead the horticultural plants in an environment that requires solving problems | 0 |
| PLO08 | - | Access to resources related to garden plants, benefiting from these resources and self-renewal | 0 |
| PLO09 | - | To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process | 0 |
| PLO10 | - | To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools | 0 |
| PLO11 | - | To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants | 0 |
| PLO12 | - | To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values. | 0 |
| PLO13 | - | To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective | 0 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Definition and application of biotechnology of plant biotechnology | Reading the related lecture notes | |
| 2 | Preparation of the solutions | Reading the related lecture notes | |
| 3 | Use of microscope, centrifuge, PH meter in biotechnology | Reading the related lecture notes | |
| 4 | Conditions laboratory of plant tissue cultures and sterilization | Reading the related lecture notes | |
| 5 | Plant tissue cultures | Reading the related lecture notes | |
| 6 | Preparation of liquid-solid LB medium, creation of a single colony of bacteria | Reading the related lecture notes | |
| 7 | Agrobacterium tumefaciens mediated gene transfer | Reading the related lecture notes | |
| 8 | Mid-Term Exam | Preparation of the exam | |
| 9 | DNA isolation and determination of quality and quantity | Reading the related lecture notes | |
| 10 | RNA and mRNA isolation, cDNA synthesis and determination of the quality-quantity | Reading the related lecture notes | |
| 11 | application of PCR | Reading the related lecture notes | |
| 12 | Agarose gel electrophoresis | Reading the related lecture notes | |
| 13 | Polyacyrilamide gelelectrophoresis | Reading the related lecture notes | |
| 14 | DNA bands imaging methods in polyacrylamide gel | Reading the related lecture notes | |
| 15 | DNA sequencing | Reading the related lecture notes | |
| 16 | Term Exams | Preparation of the exam | |
| 17 | Term Exams | Preparation of the exam |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||