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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| HORTICULTURAL CROPS (PhD) | |
| Code | BB614 |
| Name | Methods for Identifying Genes and Gene Expression in Plants |
| Term | 2019-2020 Academic Year |
| Term | Spring |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of this course is to the teach methods for identifying genes and gene expression in plants.
Course Content
The contents of this course include structure of nucleic acids, protein translation and proteins, mechanism of transcription in plants and protein translation, gene-protein (enzyme), gene-RNA conversions, promotors and determination of promotors by PCR and sequencing techniques in plants, gene banks for promoters, RT PCR analysis to determine the presence of mRNA, determination of the amount of gene expression by qRT-PCR analysis and evaluation of results, determination of gene expression by micro-array chips, and the creation of micro-array chips, bioinformatics.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications |
| LO02 | Understanding the horticulture and the interaction between the related disciplines |
| LO03 | Solving problems by research methods and establishing cause-effect relationship |
| LO04 | Using the theoretical and practical knowledge of Horticulture, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis. |
| LO05 | Carrying out a study individually that requires professional knowledge in Horticulture |
| LO06 | Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results |
| LO07 | Leading in the situations when solving problems related to horticulture is necessary |
| LO08 | Mid-Term Exam |
| LO09 | Entering database related to horticulture, utilizing those sources and self-updating |
| LO10 | Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process |
| LO11 | Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments |
| LO12 | Using software related to horticulture sufficiently and using information and communication technologies in advanced level |
| LO13 | Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values |
| LO14 | Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating |
| LO15 | Using the assimilated horticultural knowledge in interdisciplinary studies, explaining, presenting and evaluating |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | To be able to develop, deepen, and to reach the original definitions that will bring innovation to the field with the original thinking and/or research of the current and advanced knowledge in his / her field based on his / her master's qualifications. | 0 |
| PLO02 | - | Comprehends interdisciplinary interaction related to horticultural plants; achieves original results using information that requires expertise in analyzing, synthesizing and evaluating new and complex ideas | 0 |
| PLO03 | - | Evaluates and uses new information in his / her field with a systematic approach | 0 |
| PLO04 | - | Can develop a new idea, method, design and/or application, or apply a known idea, method, design and/or application to a different field; can research, comprehend, design and apply an original topic | 0 |
| PLO05 | - | Make critical analysis, synthesis and evaluation of new and complex ideas | 0 |
| PLO06 | - | To be able to use research methods in his / her field of study | 0 |
| PLO07 | - | To develop a new idea, method, design and/or application, or apply a well-known idea, method, design and/or application to a different field independently of the original work to contribute to the progress in the field | 0 |
| PLO08 | - | To be able to publish at least one scientific article in national and international refereed journals, or to produce or interpret an original work, to be able to extend the limits of knowledge in the field. | 0 |
| PLO09 | - | To Be able to lead in environments that require solving interdisciplinary problems, use problem solving skills in interdisciplinary studies | 0 |
| PLO10 | - | Develops new ideas and methods related to his / her field by using high-level mental processes such as creative and critical thinking, problem solving and decision making | 0 |
| PLO11 | - | In the national and international platforms, they present original views in the discussion of issues related to the field of local and foreign experts, they can defend these views, examine, develop and change the norms leading to social relations and these relations from a critical point of view; they can communicate effectively showing their competence in the field. | 0 |
| PLO12 | - | Specialized in special topics in his / her field | 0 |
| PLO13 | - | To be an information society in which the society lives, to be able to maintain it, to contribute to the solution of the social, scientific, cultural and ethical problems encountered. | 0 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Structure of nucleic acids | Reading the related lecture notes | |
| 2 | Translation and proteins | Reading the related lecture notes | |
| 3 | Mechanism of transcription and translation in plants | Reading the related lecture notes | |
| 4 | Gene-protein (enzyme), gene-RNA conversions | Reading the related lecture notes | |
| 5 | Gene-protein (enzyme), gene-RNA conversions | Reading the related lecture notes | |
| 6 | Promotors and determination of promotors by PCR and sequencing techniques in plant | Reading the related lecture notes | |
| 7 | Promotors and determination of promotors by PCR and sequencing techniques in plants | Reading the related lecture notes | |
| 8 | Mid-Term Exam | Preparation of the exam | |
| 9 | Gene banks for promoters | Reading the related lecture notes | |
| 10 | Gene banks for promoters | Reading the related lecture notes | |
| 11 | RT PCR analysis to determine the presence of mRNA | Reading the related lecture notes | |
| 12 | Determination of the amount of gene expression by qRT-PCR analysis and evaluation of results | Reading the related lecture notes | |
| 13 | Determining of gene expression by microarray chips, and the creation of microarray chips | Reading the related lecture notes | |
| 14 | Determining of gene expression by microarray chips, and the creation of microarray chips | Reading the related lecture notes | |
| 15 | Bioinformatics | Reading the related lecture notes | |
| 16 | Term Exams | Preparation of the exam | |
| 17 | Term Exams | Preparation of the exam |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 152 | ||
| Total Workload / 25 (h) | 6,08 | ||
| ECTS | 6 ECTS | ||