TÜRKÇE
ENGLISH
Information
| Unit | FACULTY OF AGRICULTURE |
| FIELD CROPS PR. | |
| Code | TBP354 |
| Name | Tissue Culture Techniques |
| Term | 2019-2020 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Üniversite Dersi |
| Type | Normal |
| Label | E Elective |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. CELALEDDİN BARUTÇULAR |
| Course Instructor |
Prof. Dr. RÜŞTÜ HATİPOĞLU
(Bahar)
(A Group)
(Ins. in Charge)
Prof. Dr. HAKAN ÖZKAN (Bahar) (A Group) (Asst. Ins.) |
Course Goal / Objective
The students learn the basic principles of tissue culture and the tissue culture techniques used in the plants
Course Content
Definition of tissue culture, organization of tissue culture laboratory, preparation of medium and stelization techniques, embryogenesis. organogenesis, tissue cultue techniques used in the plants
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns tools and equipment required in the tissue culture laboratory |
| LO02 | Learns the principles of sterile working |
| LO03 | Learns medium preparation for tissue culture |
| LO04 | Learns isolation and culture of plant explant |
| LO05 | Learns processes of tissue culture techniques |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | Has knowledge about agricultural engineering as well as agronomy and breeding of field crops. | 4 |
| PLO02 | - | Determines and solves the problems related to agricultural engineering as well as agronomy and breeding of field crops. | 4 |
| PLO03 | - | Graduates gain abilty to synthetize the basic concepts related to the field crops. | 4 |
| PLO04 | - | Rrecognises problems related to agricultural engineering,makes decisions and takes initiative to solve the problems. | 2 |
| PLO05 | - | Gains knowledge about sustainable agriculture, protection of environment and natural sources, biodiversity and conservation of genetic sources. | 4 |
| PLO06 | - | Gains ability to optimize the plant production by sustainable use of natural resources. | 4 |
| PLO07 | - | Learns basic principles of breeding and biotechnology of field crops. | 5 |
| PLO08 | - | Chooses and uses modern technical equipments for the agricultural engineering applications as well as for the applications in the agronomy and breeding of field crops. | 4 |
| PLO09 | - | Gains ability to establish suitable research experiments for the purpose and the ability to interpret its results by scientific methods. | 3 |
| PLO10 | - | Works both individually and in a team. | 1 |
| PLO11 | - | Evaluates the learned knowledge by analytical and critical approach. | 3 |
| PLO12 | - | Internalizes the necessity of lifelong learning. | 1 |
| PLO13 | - | Has an effective and healthy communication in his fıeld and use communication technologies. | 1 |
| PLO14 | - | Improve themselves consistently by determining educational requirements in scientific, cultural and social areas depending on their abilities,besides their career development | 0 |
| PLO15 | - | Shows respect to job ethic. | 2 |
| PLO16 | - | Becomes competent in the legislation and management systems related to agricultural engineering. | 3 |
| PLO17 | - | Becomes proficient in doing, applying, managing and monitoring plans and projects about agricultural engineering | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to tissue culture laboratory and its organization. | Reading lecture topic from the course book and the articles about the topic | |
| 2 | Components of the nutrient medium for tissue culture, chemical used, properties of the chemicals used in tissues culture | Reading lecture topic from the course book and the articles about the topic | |
| 3 | Solution preparation techniques in tissue culture | Reading lecture topic from the course book and the articles about the topic | |
| 4 | Solution preparation techniques in tissue culture Preparation of stock solutions for mineral elements | Reading lecture topic from the course book and the articles about the topic | |
| 5 | Preparation of stock solutions (vitamins, hormone etc) | Reading lecture topic from the course book and the articles about the topic | |
| 6 | Techniques of sterilization in tissues culture and homework will be given on this topic | Reading lecture topic from the course book and the articles about the topic | |
| 7 | Techniques of medium preparation | Reading lecture topic from the course book and the articles about the topicReading lecture topic from the course book and the articles about the topic | |
| 8 | Mid-Term Exam | Preparation to mid-term exam | |
| 9 | Sterilization of plant materials in tissues culture, isolation of explant and its culture | Reading lecture topic from the course book and the articles about the topic | |
| 10 | Plant factors affecting succes of tissue culture | Reading lecture topic from the course book and the articles about the topic | |
| 11 | Definition, application and uses of embryo culture | Reading lecture topic from the course book and the articles about the topic | |
| 12 | Meristem culture | Reading lecture topic from the course book and the articles about the topic | |
| 13 | Callus ,cell and protoplast culture | Reading lecture topic from the course book and the articles about the topic | |
| 14 | Techniques of obtaining haploid plants in vitro | Reading lecture topic from the course book and the articles about the topic | |
| 15 | Somaclonal variation and somatic hybridization | Reading lecture topic from the course book and the articles about the topic | |
| 16 | Term Exams | Preparation to final examination | |
| 17 | Term Exams | Preparation to final examination |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Homework | 100 | 20 |
| General Assessment | ||
| Midterm / Year Total | 100 | 20 |
| 1. Final Exam | - | 80 |
| Grand Total | - | 100 |