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Information
| Code | ECF301 |
| Name | Biochemistry Laboratory |
| Semester | 5. Semester |
| Duration (T+A) | 0-3 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 1 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. ÖZLEM ALPTEKİN |
Course Goal
To inform the students about the methods of biochemical analysis and to give the laboratory skills about the application of basic biochemical analysis techniques. At the end of the course students are expected to acquire skills in basic biochemical analysis techniques and applications.
Course Content
Qualitative and quantitative determinations of carbohydrates, lipids and proteins, as well as calorie quantitative sugar and protein identification; protein identification in urine; serum lipid analysis, total lipid and cholesterol determination, renal function tests, urine and serum creatinine assay; liver function tests; transaminases, bilirubin metabolism; lactate dehydrogenase enzyme design; immunoassay model application and immunodiffusion test; Analysis of proteins by PAGE and SDS-PAGE.
Course Precondition
There is no prerequisite.
Resources
Alptekin Ö. Biyokimya Laboratuvar Föyü, Eczacılık Fakültesi Yayınları, Yayın No:7, 2018, Adana.
Notes
Alptekin Ö, lecture notes uploaded to ÇÜBİS system, 2022
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | She/He says the general rules in the biochemistry laboratory. |
| LO02 | She/He explains how to use the devices in the laboratory. |
| LO03 | She/He systematically qualitatively examines a mixed sample for carbohydrate content and interprets its content. |
| LO04 | She/He explains the principles of blood sugar determination methods. |
| LO05 | She/He explains the connection between fasting blood sugar level and diabetes. |
| LO06 | She/He explains the qualitative analysis steps of amino acids. |
| LO07 | She/He says about serum proteins. |
| LO08 | She/He says general protein determination methods. |
| LO09 | She/He says some pathological conditions in which total protein and albumin levels increase and decrease. |
| LO10 | She/He explains the relationship between creatinine clearance and early renal failure. |
| LO11 | She/He determines Hemoglobin spectrophotometrically. |
| LO12 | She/He draws a calibration curve from standard cholesterol spectrophotometrically. |
| LO13 | She/He explains the principle of immunoassay techniques. |
| LO14 | She/He makes the determination of albumin in the urine using the ELISA method. |
| LO15 | She/He determines DNA purity spectrophotometrically. |
| LO16 | She/He recognizes and says the parts of the electrophoresis device. |
| LO17 | She/He calculates the molecular mass of bovine serum albumin by electrophoresis. |
| LO18 | She/He measures serum LDH activity in the laboratory. |
| LO19 | She/He gains research skills. |
| LO20 | She/He recognizes the material and device used in the analysis method. |
| LO21 | She/He uses appropriate language and format in the report. |
| LO22 | She/He gains individual management skills. |
| LO23 | She/He uses decision making skills. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have sufficient knowledge in pharmaceutical field and develop the knowledge. | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends professional knowledge with ethical responsibility through current laws and legislation. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Provides consultancy service to patients, community and health care personnel and evaluates advanced knowledge and skills with a critical approach. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Possessing the knowledge of historical and current developments in the field of Pharmacy, monitoring, evaluation and application of scientific and evidence-based data, and adopting lifelong learning. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Have the knowledge and skills to help provide patient-oriented pharmacy applications and individualized pharmaceutical care by learning the basics of Clinical Pharmacy, | 2 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes a role in the evaluation and analysis of the events and cases related to the development of pharmacy profession and education. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Integrates and develops professional knowledge via different disciplines and uses the acquired knowledge to improve the health level and quality of life of the community. | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Use effectively the concepts and applications of basic sciences and basic medical sciences in the field of medicine and pharmacy and update the information. | 5 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicates effectively in writing and speaking. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Practices the profession in accordance with Atatürk's principles and reforms, equipped with universal knowledge, uses Turkish correctly and knows at least one foreign language. | |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Be an example to society withexternal appearance, demeanor, attitude and behavior for the other professionals and society and with professional identity | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | Have knowledge about health policies and health economics, pharmacy management and define the problems in pharmaceutical field. | 2 |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | Interprets information about current health and treatment problems and attends meeting on related matters . Plays an active role in community health. | 2 |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Acquires basic information about diseases, makes decisions about the drugs used in treatment in accordance with rational drug use. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General information about the laboratory | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası, Deney / Laboratuvar, Bireysel Çalışma, Problem Çözme |
| 2 | Qualitative tests of carbohydrates | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar, Bireysel Çalışma, Problem Çözme |
| 3 | Determination of fasting blood sugar | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 4 | Qualitative tests of amino acids | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar, Bireysel Çalışma, Problem Çözme |
| 5 | Qualitative tests of proteins | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Deney / Laboratuvar, Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Bireysel Çalışma |
| 6 | Determination of total protein and albumin in serum | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar, Bireysel Çalışma, Problem Çözme |
| 7 | Quantitation of creatinine in serum | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Deney / Laboratuvar, Bireysel Çalışma, Problem Çözme |
| 8 | Mid-Term Exam | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Determination of Hemoglobin in Blood | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar, Problem Çözme |
| 10 | Determination of total cholesterol | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | DNA isolation methods | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası, Deney / Laboratuvar |
| 12 | Separation of proteins by PAGE electrophoresis | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Deney / Laboratuvar |
| 13 | Determination of albumin in the urine by ELISA | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Bireysel Çalışma, Problem Çözme, Deney / Laboratuvar |
| 14 | Determination of Lactate Dehydrogenase Activity in Serum | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Deney / Laboratuvar, Problem Çözme |
| 15 | Determination of alkaline phosphatase activity in serum | The relevant experiment is studied from the laboratory booklet. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Bireysel Çalışma, Problem Çözme |
| 16 | Term Exams | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Yazılı Sınav, Performans Değerlendirmesi |
| 17 | Term Exams | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Yazılı Sınav, Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 3 | 3 |
| Final Exam | 1 | 9 | 9 |
| Total Workload (Hour) | 54 | ||
| Total Workload / 25 (h) | 2,16 | ||
| ECTS | 2 ECTS | ||