TÜRKÇE
ENGLISH
Information
| Code | VTS206 |
| Name | Analytical Methods in Pharmacology and Toxicology |
| Term | 2022-2023 Academic Year |
| Semester | 4. Semester |
| Duration (T+A) | 1-0 (T-A) (17 Week) |
| ECTS | 1 ECTS |
| National Credit | 1 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. PETEK PİNER BENLİ |
| Course Instructor |
Prof. Dr. PETEK PİNER BENLİ
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
The aim of this course is to teach students the existence and quantity of medicines / poisons affecting the body systems and the theoretical and practical knowledge of the laboratory devices and their principles used for this purpose.
Course Content
Basic laboratory tool and their usage principles, the usege and working principle of spectrophotometry, steps of sample preparation in chromatographic analyzes and its types, genotoxicity and its measurement
Course Precondition
None
Resources
İlaç ve Zehir Laboratuvar Uygulama Kitabı, Abdullah Doğan, 2019
Notes
Kromatografik Yöntemler, Ayla Demirci, Mustafa Özcimder, 2019. Enstrümantal Analiz İlkeleri, Esma Kılıç, Hamza Yılmaz, 2013 Zehirli Maddelerin Laboratuvar Analizi, Sezai Kaya, 2012
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explains basic laboratory tools and equipment, their use and working principles. |
| LO02 | Explains the use and working principle of spectrophotometry. |
| LO03 | List the basic principles used in chromatographic analysis. |
| LO04 | Explains the purpose for which Atomic Absorption Spectroscopy technique is used and its application method. |
| LO05 | List the stages of fluorescence spectroscopy technique used in drug analysis. |
| LO06 | Explain sample preparation methods in chromatographic analysis. |
| LO07 | Express how to use HPCL technique in pharmacological and toxicological analysis. |
| LO08 | Explains how to make qualitative and quantitative analyzes of drugs and toxic substances using GC technique. |
| LO09 | Shows parts of HPLC and GC devices and explains how to use them. |
| LO10 | Explains the application areas of Thin Layer Chromatography technique in pharmacology and toxicology with examples. |
| LO11 | Genotoxicity tests and Comet method explain in their own words for what purposes and how to apply. |
| LO12 | Express the stages of ELISA Technique and how it is applied. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Learns animal species and breeds, recognizes structural, functional and behavioral characteristics (anatomical, histological, physiological, biochemical, etc.). | |
| PLO02 | Bilgi - Kuramsal, Olgusal | It determines the care and nutrition alternatives for healthy aquaculture and prepares feeding programs suitable for the purpose in accordance with the cultivation patterns and expected yield characteristics of different animal species. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Determine the diagnosis and treatment protocols of diseases. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Learns basic information about chemical and biological substances to be used for prevention and treatment of diseases and their production technologies. | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Evaluates the efficiency of animal breeds. Learns the level of animal breeding. Able to manage an animal selection, hybridization and artificial insemination program. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Makes the most appropriate management planning for livestock enterprises. | |
| PLO07 | Bilgi - Kuramsal, Olgusal | Has knowledge about animal rights and animal owner rights. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has sufficient technology knowledge about hygienic production, production, presentation and sales of animal foods produced for human consumption and / or products of animal products. | 4 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Learns national and international laws and regulations related to veterinary medicine. | 3 |
| PLO10 | Bilgi - Kuramsal, Olgusal | A veterinary practitioner manages animal husbandry enterprises, animal husbandry scientific research centers and animal food production enterprises by utilizing the knowledge gained in the veterinary medicine education and by observing the professional principles. | 2 |
| PLO11 | Bilgi - Kuramsal, Olgusal | Recognizes all kinds of diseases that are possible in animals, uses diagnostic methods for this purpose, receives diagnostic materials if necessary and sends them to authorized laboratories and treats the diseases diagnosed by using all methods of medicine (such as interpretation of diagnostic methods results, drug or operative applications). It takes necessary security measures including biosecurity for the control of infectious diseases. | |
| PLO12 | Bilgi - Kuramsal, Olgusal | Prepares and uses the theoretical maintenance-feed protocols. Makes necessary applications for economic aquaculture by considering the characteristics at the business and regional level. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Inspects the production of animal foods and products of animal origin which produced for human consumption and performs health control of the final foodstuffs. | 5 |
| PLO14 | Bilgi - Kuramsal, Olgusal | Being aware of the duties and responsibilities to have the consciousness of exercising the profession. Takes responsibility in individual and group work, fulfills the task in line with the skills. | 4 |
| PLO15 | Bilgi - Kuramsal, Olgusal | In cooperation with other experts in problems outside the field of expertise, the team contributes to the solution as a member. Also trying to find solutions to common problems at the regional or national level as well as communication with other professionals. | 3 |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Comprehends the methods of producing and using scientific knowledge. Evaluates the knowledge and skills gained in a critical way, identifies the new information and information sources that should be acquired in relation to the field and participates in the activities that will meet the learning needs. | 4 |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes tasks related to the field in professional councils and organizations. Based on his professional skills and competences, he plans and realizes social projects with the awareness of society. Provides information on the problems of public and animal health related to the definition of the problem and the solution proposals and organizes events. | 3 |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | He / she can continue his / her professional development and use at least one foreign language (at least European Language Portfolio B1 general level) to establish social relations. At least at the Advanced Level of European Computer Driving License, it uses computer software and other technological tools that may be needed at professional level and social relations level. He follows the world agenda closely and is sensitive to events. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Be aware of the needs of the country and the region, and if necessary, guide them in their practice. It defines the subject of human, animal and animal owner rights and considers animal welfare in all its practices. They have the ethical values that are required by the profession, and defend these values when necessary. Have the competence to practice and defense, dominate the concepts of occupational safety in terms of work place and the personnel working with it. It is aware of the environmental health rules and makes practices to protect nature. The environment is sensitive to veterinary public health and zoonoses. | 3 |
| PLO20 | Yetkinlikler - Öğrenme Yetkinliği | Conceptualize the facts and events related to veterinary services; examine scientific techniques and methods, interpret the results; constructs analysis or methods for problems; provides solutions and / or treatment alternatives according to the data obtained. Follows the legislation that is updated on veterinary services and uses them when necessary. | 3 |
| PLO21 | Bilgi - Kuramsal, Olgusal | Evaluate the physiological and physiopathological conditions of animals conceptually and factually. | |
| PLO22 | Yetkinlikler - Alana Özgü Yetkinlik | Evaluates the laboratory results (hematological, biochemical, EKG, EEG, EMG, etc.) of animal diseases and applies correct diagnosis, diagnosis and treatment methods based on the data obtained. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction, Basic laboratory safety procedures, Basic laboratory tools and equipment | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Spectrophotometric Methods | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Atomic Absorption Spectroscopy | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Fluorescence and Phosphorescence Spectroscopy | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Basic Principles of Chromatography | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Thin Layer Chromatography (TLC) -I | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Thin Layer Chromatography (TLC) -II | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Study lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | High Performance Liquid Chromatography (HPLC) -I | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | High Performance Liquid Chromatography (HPLC) -II | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Gas Chromatography (GC) -I | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Gas Chromatography (GC) -II | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Comet Assay-I | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Comet Assay-II | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | ELISA | Study lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Study lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Study lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 1 | 14 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 4 | 4 |
| Total Workload (Hour) | 34 | ||
| Total Workload / 25 (h) | 1,36 | ||
| ECTS | 1 ECTS | ||