TÜRKÇE
ENGLISH
Information
| Code | BB614 |
| Name | Methods for Identifying Genes and Gene Expression in Plants |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to the teach methods for identifying genes and gene expression in plants.
Course Content
The contents of this course include structure of nucleic acids, protein translation and proteins, mechanism of transcription in plants and protein translation, gene-protein (enzyme), gene-RNA conversions, promotors and determination of promotors by PCR and sequencing techniques in plants, gene banks for promoters, RT PCR analysis to determine the presence of mRNA, determination of the amount of gene expression by qRT-PCR analysis and evaluation of results, determination of gene expression by micro-array chips, and the creation of micro-array chips, bioinformatics.
Course Precondition
No
Resources
Notes and presentations prepared within the scope of the course content.
Notes
Molecular Biology Mehmet KARATAŞ Regulation of Gene Expression Editors: Gary H. Perdew, John P. Heuvel, Jeffrey M. Peters
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows nucleic acids and their structure, protein synthesis. |
| LO02 | Explain and interpret the transcription and translation stages in order to understand gene expression techniques in plants. |
| LO03 | Explain the gene-protein relationships. |
| LO04 | Knows PCR technology and detecting gene expressions with RT PCR. Can compare different gene expression techniques. |
| LO05 | To be able to use, explain, transfer to others the information assimilated in the field of Plant Molecular Genetics in interdisciplinary studies, and examine the results with a critical perspective. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To be able to develop, deepen, and to reach the original definitions that will bring innovation to the field with the original thinking and/or research of the current and advanced knowledge in his / her field based on his / her master's qualifications. | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends interdisciplinary interaction related to horticultural plants; achieves original results using information that requires expertise in analyzing, synthesizing and evaluating new and complex ideas | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Evaluates and uses new information in his / her field with a systematic approach | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Can develop a new idea, method, design and/or application, or apply a known idea, method, design and/or application to a different field; can research, comprehend, design and apply an original topic | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Make critical analysis, synthesis and evaluation of new and complex ideas | 1 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To be able to use research methods in his / her field of study | 2 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To develop a new idea, method, design and/or application, or apply a well-known idea, method, design and/or application to a different field independently of the original work to contribute to the progress in the field | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To be able to publish at least one scientific article in national and international refereed journals, or to produce or interpret an original work, to be able to extend the limits of knowledge in the field. | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To Be able to lead in environments that require solving interdisciplinary problems, use problem solving skills in interdisciplinary studies | 1 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Develops new ideas and methods related to his / her field by using high-level mental processes such as creative and critical thinking, problem solving and decision making | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | In the national and international platforms, they present original views in the discussion of issues related to the field of local and foreign experts, they can defend these views, examine, develop and change the norms leading to social relations and these relations from a critical point of view; they can communicate effectively showing their competence in the field. | 2 |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | Specialized in special topics in his / her field | 3 |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | To be an information society in which the society lives, to be able to maintain it, to contribute to the solution of the social, scientific, cultural and ethical problems encountered. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Structure of nucleic acids | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Translation and proteins | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Mechanism of transcription in plants | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Translation mechanism in plant | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Gene-protein (enzyme), gene-RNA conversions | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Promotors in Plant | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 7 | determination of promotors by PCR and sequencing techniques in plants | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Preparation of the exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Gene banks for promoters | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Microarray analyzes for generation of microarray chips and determination of gene expression | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | RT PCR analysis to determine the presence of mRNA | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 12 | Determination of the amount of gene expression by qRT-PCR analysis and evaluation of results | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 13 | DNA sequencing Technology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 14 | RNA sequencing Technology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 15 | Bioinformatics | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Preparation of the exam | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 17 | Term Exams | Preparation of the exam | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 152 | ||
| Total Workload / 25 (h) | 6,08 | ||
| ECTS | 6 ECTS | ||