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Information
| Code | BY707 |
| Name | PAGE Tecniques |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. HATİCE GÜVENMEZ |
| Course Instructor |
1 |
Course Goal / Objective
To teach protein electrophoresis techniques used in molecular biology applications
Course Content
Isolation of proteins from living tissues, solutions for isolation, electrophoresis techniques and applications
Course Precondition
None
Resources
Protein Methods
Notes
Current Literatures
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Comprehend the importance of Page |
| LO02 | Understands the structure and formation of polyacrylamide gels |
| LO03 | Comprehend the usage areas and purposes of polyacrylamide gel systems |
| LO04 | comprehend the application of PAGE techniquee |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Identifies the interdisciplinary interaction that the field is related to. | 4 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Interprets the knowledge gained in the field by integrating the information from different disciplines and creates new information. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Carries out a study that requires expertise related to the field independently. | 4 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Evaluates the knowledge and skills acquired at the level of expertise in the field with a critical approach and directs its learning. | 5 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Supports the current developments in the field and their studies with quantitative and qualitative data and transmits them both in the field and outside the field systematically in written, oral and visual form. | 4 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | It supervises the social, scientific, cultural and ethical values in the collection, interpretation, application and announcement stages of the data related to the field and teaches these values. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | The information they absorbed in the field; use problem solving and / or practical skills in interdisciplinary studies. | 3 |
| PLO09 | Belirsiz | It contributes to the solution of social, scientific, cultural and ethical problems encountered in the field and supports the development of these values. | |
| PLO10 | Belirsiz | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | The buffers for proteins isolation | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 2 | Salts, Metal ions, Inhibitors and chelators | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 3 | proteinase inhibitors | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 4 | PAGE structure and formation | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Polymerization and catalizors | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Soru-Cevap, Gösterip Yaptırma |
| 6 | Physicochemical properties of gel forming components | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım |
| 7 | Preparation of separating and stacking gels | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Deney / Laboratuvar |
| 8 | Mid-Term Exam | An overview | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
| 9 | Preparation of the homogen, gradient, denature and native PAGE and running the protein samples | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Causes of errors in PAGE electrophoresis applications | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım |
| 11 | Detection methods of protein bands | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 12 | Cellulose asetate electrophoresis | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 13 | Isoelectric focusing | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 14 | Detection of enzyme activity in PAGE | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 15 | Drying the acriylamide gels and hiding. | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 16 | Term Exams | An overview | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
| 17 | Term Exams | oral examination | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 4 | 6 | 24 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||