BT505 Basic Principles of Gene Cloning

6 ECTS - 2-0 Duration (T+A)- . Semester- 2 National Credit

Information

Code BT505
Name Basic Principles of Gene Cloning
Term 2022-2023 Academic Year
Semester . Semester
Duration (T+A) 2-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 2 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. BURHAN ARIKAN


Course Goal / Objective

Gene Cloning and its applications

Course Content

The Importance of Gene Cloning, Properties and Functions of Plasmid and Bacteriophages, Isolation and Purification of Chromosomal, Pazmid and Bacteriophage DNAs from Live Organisms, Assignment of DNA Using Transformational Systems to Live Organisms and the Properties of Techniques Used in Cloning. Polymerase chain reaction, Microbiology, cloning in agriculture and medicine

Course Precondition

none

Resources

Gene cloning and DNA analyses

Notes

Gene applications


Course Learning Outcomes

Order Course Learning Outcomes
LO01 To be able to evaluate the knowledge and skills acquired in the field with a critical approach and to direct the learning
LO02 To be able to transfer the current developments and his / her studies to the groups in the field and outside of the field by supporting them with quantitative and qualitative data systematically.
LO03 To be able to follow international studies related to the field, to take part in various social interaction and scientific working groups at international level and to have communication skills in at least one foreign language in order to share their studies at international level
LO04 To be able to use computer software and information and communication technologies at a sufficient level required by the field of biotechnology
LO05 Ability to use the knowledge, problem solving and / or application skills in interdisciplinary studies
LO06 To be able to deepen the general knowledge of biotechnology science within the framework of basic theories and applications in the field of study
LO07 Being aware of social, scientific and ethical values in the studies conducted in the field; to carry out the research process within the framework of these values
LO08 To be able to critically evaluate assumptions, thoughts and theories related to biotechnology with a skeptical, logical, analytical and independent perspective.
LO09 Comprehensive knowledge about modern techniques and methods applied in natural and applied sciences and their boundaries
LO10 To be able to develop and deepen knowledge in the field of biotechnology
LO11 To be able to comprehend the interdisciplinary interaction related to biotechnology
LO12 Ability to interpret and integrate the knowledge gained in biotechnology with information from different disciplines and to create new knowledge
LO13 To be able to solve problems encountered in the field by using research methods
LO14 To be able to conduct an independent study related to the field
LO15 To be able to develop new strategic approaches to solve unforeseen complex problems encountered in applications related to biotechnology and to be able to produce solutions by taking responsibility


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods.
PLO02 Beceriler - Bilişsel, Uygulamalı To identifiy the interdisciplinary interaction of the field and to solve use of the methods. 2
PLO03 Beceriler - Bilişsel, Uygulamalı Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information 3
PLO04 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Develops new strategic approaches for solving complex problems that are encountered in applications related to the field.
PLO05 Yetkinlikler - Öğrenme Yetkinliği To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. 4
PLO06 Yetkinlikler - İletişim ve Sosyal Yetkinlik To solve the problems encountered in the field and cominicate by using research methods.
PLO07 Yetkinlikler - Alana Özgü Yetkinlik To gain the ability to develop and deepen the knowledge in the field of biotechnology 2
PLO08 Yetkinlikler - Alana Özgü Yetkinlik Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field.
PLO09 Yetkinlikler - Öğrenme Yetkinliği Knows the ethical rules to be considered while obtaining biotechnological products.
PLO10 Yetkinlikler - İletişim ve Sosyal Yetkinlik He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field.


Week Plan

Week Topic Preparation Methods
1 Importance of Gene Cloning, Study related topics Öğretim Yöntemleri:
Anlatım
2 Plasmide Study related topics Öğretim Yöntemleri:
Anlatım
3 Bakteriophage Study related topics Öğretim Yöntemleri:
Anlatım
4 Chromosomal DNA, plasmid and bacteriophage isolation techniques from bacteria Study related topics Öğretim Yöntemleri:
Anlatım, Beyin Fırtınası
5 Chromosomal DNA isolation techniques from plants Study related topics Öğretim Yöntemleri:
Anlatım
6 Chromosomal DNA isolation methods from yeast Study related topics Öğretim Yöntemleri:
Anlatım
7 Chromosomal DNA isolation methods from fungi Study related topics Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam Ölçme Yöntemleri:
Ödev
9 Conjugation Study related topics Öğretim Yöntemleri:
Anlatım
10 Transformation Study related topics Öğretim Yöntemleri:
Anlatım
11 Electroporation Study related topics Öğretim Yöntemleri:
Anlatım
12 Electrofusion, biolistic transformation and liposome transformation Study related topics Öğretim Yöntemleri:
Anlatım
13 Cloning vectors Study related topics Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
14 Recombinant DNA production and transfer to the recipient organism Study related topics Öğretim Yöntemleri:
Anlatım, Tartışma
15 Polymerase chain reaction and applications Study related topics Öğretim Yöntemleri:
Anlatım, Tartışma, Gösterip Yaptırma
16 Term Exams Ölçme Yöntemleri:
Sözlü Sınav, Ödev, Performans Değerlendirmesi
17 Term Exams Ölçme Yöntemleri:
Sözlü Sınav, Ödev, Performans Değerlendirmesi


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 2 28
Out of Class Study (Preliminary Work, Practice) 14 6 84
Assesment Related Works
Homeworks, Projects, Others 2 6 12
Mid-term Exams (Written, Oral, etc.) 1 6 6
Final Exam 1 12 12
Total Workload (Hour) 142
Total Workload / 25 (h) 5,68
ECTS 6 ECTS

Update Time: 24.11.2022 12:02