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Information
| Code | BT077 |
| Name | Immunohistochemical and Immunocytochemical Techniques in Animal Tissues |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MEHMET HALIGÜR |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to determine the reactions which can be determined by immunohistochemical method in animal tissues or animal cells.
Course Content
In this course, antigen-antibody complexes in animal tissues, immunohistochemical or immunocytochemical detection of infectious agents and tumor markers, immunohistochemistry and immunocytochemistry method, types and applications and immunohistochemical characteristics will be given.
Course Precondition
None
Resources
Lin F, Prichard J. 2015. Handbook of Practical Immunohistochemistry, Springer; Dabbs D. 2021. Diagnostic Immunohistochemistry, 6th Edition, Elsevier.
Notes
Lecture notes and current literature
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Students will learn to apply immunohistochemical methods in animal tissues |
| LO02 | Learns what can be done in tissues by immunohistochemical method |
| LO03 | In experimental studies, how to determine the infectious diseases and determine the molecular changes in tissues |
| LO04 | Learns determination of proteins or receptors in tissues |
| LO05 | Identify antigen-antibody complexes and make them visible or detectable by light microscope |
| LO06 | Learn the types and stages of immunohistochemical and immunocytochemical methods |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 3 |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 5 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 4 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 2 |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 3 |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 2 |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 5 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Definition of immunohistochemistry, general feature, what is included in the content | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 2 | Definition of immunohistochemistry and the properties and types of hyperimmune sera | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 3 | The application areas of immunohistochemistry, how to apply to tissues, how to apply to cytological preparations | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 4 | The application areas of immunohistochemistry, what are the considerations when touching, what are the considerations when applying to cytological preparations. | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 5 | Types of immunohistochemistry and immunocytochemistry, PAP and APPAP methods, how to apply | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 6 | immunohistochemically identification of infectious agents, in particular bacteria such as actinomyces pyognes, which cause pathological effects in animal tissues, identification of intercellular bacteria such as mycobacterium | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 7 | immunohistochemically determining cell receptors; The role of immunohistochemistry in the detection and detection of receptors such as TNF-alpha, Fas-Ligand | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 8 | Mid-Term Exam | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 9 | Determination of cell death pathways by immunohistochemical method, mechanism of action of caspases, effect of caspases on cell death | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Use of tumor cell markers as immunohistochemically; PCNA refers to Ki-67, which markers are used in epithelial tumors | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 11 | The use of tumor cell markers in immunocytochemistry, which markers are used in mesenchymal origin tumors, which markers are used in smears, | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 12 | The markers used in the application of immunohistochemical methods, markers used in gland tumors, the markers of hemotopeoietic system cells | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 13 | The markers used in the application of immunohistochemical method; What are the ingredients used in bone tissue and tu | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 14 | Immunohistochemistry and immunocytochemistry application; application stages, what are the points to be considered at these stages and the solution of the problems related to these stages, | Lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme |
| 15 | Immunohistochemistry and immunocytochemistry application; ways to solve problems, evaluation of preparations and how to prevent incorrect evaluations | Lecture notes | Öğretim Yöntemleri: Anlatım, Problem Çözme |
| 16 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||