BT075 Laboratory Applications in Animal Biotechnology

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit
Information About Course

Information
Goal, Content & Resources
Course Learning Outcomes
Relation with Program Learning Outcome
Week Plan
Student Workload - ECTS

Information

Code BT075
Name Laboratory Applications in Animal Biotechnology
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. BAHRİ DEVRİM ÖZCAN


Course Goal

Transfer of the basic techniques used in the field of biotechnology as theoretical and practical to postgraduate students

Course Content

Considerations in laboratory studies, solution preparation, DNA and RNA isolation from procaryotes and eukaryotes, electrophoresis, purification of DNA from agarose, isolation and electrophoresis of proteins, PCR technology

Course Precondition

No prerequisite

Resources

Animal Biotechnology, 2018. A.K. Srivastava, R.K. Singh, M.P. Yodav, CBS PUB & DIST PVT LTD INDIA, ISBN-13 ‏ : ‎ 978-8120416482

Notes

Advances in Animal Biotechnology, 2019. B. Singh, G. Mal, S.K. Gautam, M. Mukesh, Springer, ISBN: 978-3-030-21309-1


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Use tools and equipment that can be found in a biotechnology laboratory
LO02 Prepare the media and solutions that can be used in laboratory
LO03 Isolate DNA and RNA from prokaryotic and eukaryotic organisms
LO04 Performs electrophoretic analysis of nucleic acid and protein molecules
LO05 Amplify a specific region on the DNA molecule using the PCR technique


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. 2
PLO02 Beceriler - Bilişsel, Uygulamalı To identifiy the interdisciplinary interaction of the field and to solve use of the methods.
PLO03 Beceriler - Bilişsel, Uygulamalı Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information
PLO04 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Develops new strategic approaches for solving complex problems that are encountered in applications related to the field.
PLO05 Yetkinlikler - Öğrenme Yetkinliği To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field.
PLO06 Yetkinlikler - İletişim ve Sosyal Yetkinlik To solve the problems encountered in the field and cominicate by using research methods. 2
PLO07 Yetkinlikler - Alana Özgü Yetkinlik To gain the ability to develop and deepen the knowledge in the field of biotechnology 2
PLO08 Yetkinlikler - Alana Özgü Yetkinlik Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field.
PLO09 Yetkinlikler - Öğrenme Yetkinliği Knows the ethical rules to be considered while obtaining biotechnological products.
PLO10 Yetkinlikler - İletişim ve Sosyal Yetkinlik He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field.


Week Plan

Week Topic Preparation Methods
1 General introduction of biotechnology laboratory and, rules to be followed All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
2 Bacterial media and solution preparation All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
3 Genomic DNA isolatipon from bacteria All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
4 Plasmid DNA isolation from isolation All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
5 Determination of quantity and quality of DNA molecules All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
6 Agarose gel electrophoresis All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
7 Protein isolation from bacteria All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
8 Mid-Term Exam Mid-term exam Ölçme Yöntemleri:
Yazılı Sınav
9 SDS-PAGE All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
10 DNA isolation from eukaryotic tissues All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
11 PCR and PCR types All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım
12 DNA purification from agarose gel All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
13 Nucleic acid-modifying enzymes and their use in biotechnology All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım
14 Hybridization methods 1 All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım
15 Hybridization methods 2 All biotechnology and molecular biology laboratory books Öğretim Yöntemleri:
Anlatım
16 Term Exams Term exams Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Term exams Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 2 2
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS