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Information
| Code | BTE0036 |
| Name | Introduction to Basic Molecular Biology Techniques-II |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | İngilizce |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
To learn the basic applications of molecular biology techniques in the laboratory.
Course Content
Investigation of Application Areas of Basic Molecular Biology Techniques
Course Precondition
None
Resources
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri
Notes
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Has knowledge of basic molecular biology techniques |
| LO02 | Knows in which researches the basic methods in molecular biology are used |
| LO03 | Has knowledge about the laboratory conditions under which molecular biology applications can be performed |
| LO04 | Comprehends the scientific importance of molecular biological methods |
| LO05 | Knows current molecular biological approaches and has knowledge about literature review |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 4 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 4 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 4 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Gains the ability to develop and deepen their knowledge in the field of biotechnology at the level of expertise. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Discovery of DNA and its use in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Discovery RNA and its use in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Obtaining DNA and RNA from the cell | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Obtaining protein from the cell | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Extraction of DNA and RNA from tissue | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Protein extraction from tissue | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Review of molecular biological methods in the literatureReview of molecular biological methods in the literature | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 9 | Quantification of DNA and RNA | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | PCR types and applications | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Agarose gel electrophoresis | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Eliza varieties and application method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | DNA sequencing methods and next generation sequencing | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Western blot method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Researching studies involving current molecular biological techniques | Literature browsing and reading | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 16 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||