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Information
| Code | ST011 |
| Name | Molecular Biological Methods in Aquaculture |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MAKBULE BAYLAN |
Course Goal / Objective
be teached and introduced of molecular biological methods used in aquaculture
Course Content
DNA isolation and analysis (DNA exraction of bacteria yeast and fish), Bacterial transformation (Chemical transformation, transformation by electroporation (electrotransformation)), DNA amplification by Polymerase Chain Reaction (PCR), isolation of proteins, purification and enzymatic analysis of proteins.
Course Precondition
None
Resources
Temizkan G., Arda N., 2004. Methods Used in Molecular Biology, Nobel Kitapevi, 613p
Notes
Temizkan G., Arda N., 2021. Basic and Advanced Molecular Biology Methods-Genomic and Proteomic Analysis.Nobel Kitapevi
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Ensuring do studying entirely on one s own of people wanting to join a study in genetics and biotechnology in aquaculture |
| LO02 | Have detailed information about DNA isolation |
| LO03 | Have detailed information about PCR amplification of DNA |
| LO04 | Gain knowledge of the laboratory |
| LO05 | Have knowledge about chemicals |
| LO06 | Have detailed information about the methods to be used. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Improves theoretical and practical knowledge in the field of Marine and Inland Water Biology and Fisheries Basic Sciences. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends interactions between Fisheries Basic Sciences and other disciplines. | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Determines strategies and investigates methods about their field of study in Fisheries Basic Science. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Produces new information and theories by interpreting and synthesising the information from other disciplines and uses the theoretical and practical information from their field of study in Fisheries Basic Science. | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Collects data, interprets results and suggests solutions by using dialectic research methodology in the certain field of Marine and Inland Water Biology and Fisheries Basic Sciences. | 3 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Independently plans, designs and performs a certain project in the field of Fisheries Basic Sciences. | 4 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Produces solutions by improving new strategic approaches and taking responsibilities for the potential problems in the field of study as an individual or team member. | 4 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Determines the requirements for Fishery Basic Science education, reaches the resources, critically interpretes knowledge and skills and gains experience to direct the education. | 2 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Has positive stance on the lifelong education and uses it for the public benefit by using the gained theoretical and practical knowledge in the field of Marine and Inland Water Biology and Fisheries Basic Sciences. | 1 |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Follows the current topics and improvements in the field of Fisheries Basic Sciences, publishes and presents the research results, contributes to constitution of a public conscience in the field of interest. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Effectively communicates about the field of Marine and Inland Water Biology and Fisheries Basic Sciences by using written and oral presentation tools, follows up and criticizes the meetings and seminars. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Follows up international publications and communicates with international collaborators by using language skills. | |
| PLO13 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Uses the communication and information technologies about the field of interest in an advanced level. | 4 |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Conforms, controls and teaches social, cultural and scientific ethics in the investigation and publication process of the data related with the field of interest. | 2 |
| PLO15 | Yetkinlikler - Öğrenme Yetkinliği | Improves strategies, politics and application codes by following scientific and technological developments on the certain field of Marine and Inland Water Biology and Fisheries Basic Sciences. Investigates and extends the results on behalf of public in frame of total quality management process. | 3 |
| PLO16 | Yetkinlikler - Öğrenme Yetkinliği | Uses the abilities and experiences on applications and solving problems that gained during the MSc education for the interdisciplinary studies. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA isolation and analysis, Chrosome DNA isolation | Reading at lecture note | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 2 | Chromosomal DNA isolation from bacteria | Reading at lecture note | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Chromosomal DNA isolation from yeast | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 4 | Chromosomal DNA isolation from fish | Reading at lecture note | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | transformation by chemical aplication in bacteria | Reading at lecture note | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | transformation by electroporation in bacteria | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 7 | DNA amplification by Polymerase Chain Reaction (PCR) | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | preparation for term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | formation mechanism of Polymerase Chain Reaction, basic components of PCR, functioning of PCR | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 10 | Amplification of correct pruduct with PCR, identification and analysis of PCR products | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 11 | prevention from contamination in PCR, types of PCR | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 12 | Protein isolation, purification and enzymatic analysis | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 13 | Tools, materials and chemicals | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 14 | Preparation of protein extract | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 15 | Protein extraction, storage conditions, extraction of fish tissue | Reading at lecture note | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | preparation for final exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | preparation for final exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||