TÜRKÇE
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Information
| Code | ZO027 |
| Name | Gene Cloning |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BAHRİ DEVRİM ÖZCAN |
Course Goal / Objective
To teach the importance of gene cloning, methods, agricultural and industrial applications to the students.
Course Content
The importance and purpose of gene cloning in prokaryotic organisms, DNA isolation from cell, identification of the gene to be cloned, identification of the organism for gene transfer, viruses, phages, plasmids, DNA transformation into the viable cell, determination of cloned gene in the target cell, purification of the clone, practical applications.
Course Precondition
No prerequisite
Resources
Gene Cloning and DNA Analysis: An Introduction, 8th Edition, T. A. Brown, Wiley-Blackwell, ISBN: 978-1-119-64078-3
Notes
Moleküler Biyoteknoloji, Rekombinant DNA'nın Prensipleri ve Uygulamaları, B.R. Glick, C.L. Patten, Beşinci Baskıdan Çeviri, 2021. Çeviri Editörü: T. Kankılıç, Palme Yayınları, Ankara
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Know gene cloning techniques in microorganisms |
| LO02 | Isolate the DNA and gene from organizms |
| LO03 | Knows cloning vectors, their role in cloning and how to use them |
| LO04 | Understands the biotechnological, agricultural and industrial importance of gene cloning in microorganisms |
| LO05 | Produce technological solutions to problems related to the field |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | After undergraduate education, increases knowledge in one of the fields of animal breeding and breeding, feeds and animal nutrition, biometrics and genetics. | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interaction between different disciplines | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains the ability to develop strategic approaches and produce regional, national or international solutions for the field of animal science | 1 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Gains the ability to develop knowledge with scientific methods by using the data in animal science, and to use this knowledge with the awareness of scientific, social and ethical responsibility. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Gains the ability to use and develop information technologies with computer software and hardware knowledge required by the field of animal science. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Gains the ability to convey their own studies or current developments in the field of animal science to groups in the field or other fields of science, verbally and visually. | |
| PLO07 | Bilgi - Kuramsal, Olgusal | Gains the ability to evaluate the quality processes of animal products | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Gains the ability to keep animal production dynamic in accordance with changing economic and social conditions. | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Gains the ability to follow national and international current issues, to follow developments in lifelong learning, science and technology, to constantly renew themselves and to transfer innovations to animal production. | 3 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Hayvansal ürünler ile insan sağlığı ve toplum refahı arasındaki ilişkiyi özümser |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Importance of gene cloning | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 2 | Cloning vectors | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 3 | Isolation of chromosomal and plasmid DNA from microorganisms | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 4 | DNA modifying enzymes | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 5 | Restriction endonucleases | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 6 | Compatent cell preparation | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 7 | Preparation to mid-term exam | Preparation to mid-term exam | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Mid-term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Shotgun cloning | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 10 | PCR-based cloning | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 11 | Gene transfer methods in microorganisms | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 12 | Selection and analysis of recombinant bacteria | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 13 | Biotechnologic importance of gene cloning | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 14 | Agricultural importance of gene cloning | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 15 | Industrial importance of gene cloning | 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009. | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Term exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||