TÜRKÇE
ENGLISH
Information
| Code | ZO024 |
| Name | Genetic Laboratory Applications in Livestock |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BAHRİ DEVRİM ÖZCAN |
Course Goal / Objective
To teach to the students the molecular genetic techniques applied in farm animals breading and research activities, bring application skills to them
Course Content
Use of genetic laboratory and basic principles of working in the laboratory. DNA isolation from animal tissues, PCR, electrophoresis, investigation of yield related gene regions, genetic diversity, RFLP and SNP analysis used in mutation scanning, microsatellite analysis, new generation sequencing technologies, RT-PCR applications
Course Precondition
No prerequisite
Resources
Animal Biotechnology, 2018. A.K. Srivastava, R.K. Singh, M.P. Yodav, CBS PUB & DIST PVT LTD INDIA, ISBN-13 : 978-8120416482
Notes
Advances in Animal Biotechnology, 2019. B. Singh, G. Mal, S.K. Gautam, M. Mukesh, Springer, ISBN: 978-3-030-21309-1
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows and uses the devices required in the genetic laboratory |
| LO02 | Isolates DNA and RNA from animal tissues and uses them for experimental purposes |
| LO03 | Gain the consciousness of duty and responsibility in teamwork |
| LO04 | Follow current studies about genetics and use them when necessary |
| LO05 | Generate and conduct an experiment related to genetics |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | After undergraduate education, increases knowledge in one of the fields of animal breeding and breeding, feeds and animal nutrition, biometrics and genetics. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interaction between different disciplines | 1 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains the ability to develop strategic approaches and produce regional, national or international solutions for the field of animal science | 1 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Gains the ability to develop knowledge with scientific methods by using the data in animal science, and to use this knowledge with the awareness of scientific, social and ethical responsibility. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Gains the ability to use and develop information technologies with computer software and hardware knowledge required by the field of animal science. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Gains the ability to convey their own studies or current developments in the field of animal science to groups in the field or other fields of science, verbally and visually. | |
| PLO07 | Bilgi - Kuramsal, Olgusal | Gains the ability to evaluate the quality processes of animal products | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Gains the ability to keep animal production dynamic in accordance with changing economic and social conditions. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | Gains the ability to follow national and international current issues, to follow developments in lifelong learning, science and technology, to constantly renew themselves and to transfer innovations to animal production. | 1 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Hayvansal ürünler ile insan sağlığı ve toplum refahı arasındaki ilişkiyi özümser |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Use of genetic laboratory and basic principles of working in the laboratory | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Preparation of solutions | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Isolation of nucleic acid from animal tissues | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | Electrophoresis of nucleic acids | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Electrophoresis of proteins | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 6 | Points to be considered in primer designing | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 7 | Article study | Article on the subject | Öğretim Yöntemleri: Grup Çalışması |
| 8 | Mid-Term Exam | Mid-term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Polymerase Chain Reaction (PCR) | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 10 | Determination of gene regions related to yield (theoretical) | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 11 | Mutation screening methods | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 12 | Microsatellite and its importance for livestock | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 13 | Sequencing methods (theoretical) | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 14 | Real Time PCR applications (theoretical) | Books and other publications on the subject | Öğretim Yöntemleri: Anlatım |
| 15 | Cloning in farm animals | Article on the subject | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Term exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||