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Information
| Code | HİS626 |
| Name | Histology Laboratory Techniques |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. LEMAN SENCAR GÜNDOĞDU |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to get knowledge about the basic principles and usage areas of examination techniques used in histology.
Course Content
Microscope types, light and electron microscopic tissue preparation, staining and inspection techniques and histochemical and immunohistochemical techniques.
Course Precondition
none
Resources
Michael H. Ross Histoloji Lecture and Atlas
Notes
Lecture notes and slides
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | 1- Knows the types and working principles of light and electron microscope |
| LO02 | 2-Knows the intended purpose of the light and electron microscopic solutions used in the monitoring tissue. |
| LO03 | 3- Knows the steps of light and electron microscopic tissue preperation. |
| LO04 | 4-Knows principles of tissue staining methods and principles of the histochemical staining methods. |
| LO05 | 5- Getting knowledge about immunohistochemical techniques. |
| LO06 | 6- Getting knowledge about the problems encountered in the technical steps. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have knowledge about structure and function of tissue and organ systems. | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Have knowledge about implantation biology and IVF techniques. | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains knowledge of the normal development of tissue and organ systems, developmental disorders and mechanisms. | 1 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows advanced light and electron microscopic tissue preparation, histochemical, immunohistochemistry and cell culture techniques according to the study area. | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Has knowledge about the basic properties of stem cells. | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has advanced knowledge about technological tools, devices and tools used in histological studies | 4 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical rules and ethical principles. | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has knowledge about planning of experimental studies and statistical methods. | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Prepares and examines the tissues using electron microscopic, immunohistochemistry and special staining techniques. | 5 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Usestechnological tools, devices and equipment according to the level. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Prepares experimental studies as a project. | |
| PLO12 | Bilgi - Kuramsal, Olgusal | Presents the results of research in scientific meetings and publishes them in refereed journals. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Makes critical analysis, synthesis and evaluation related to the field. | |
| PLO14 | Bilgi - Kuramsal, Olgusal | Carries out studies individually or as a team member and leads the necessary environments. | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Bilimsel bir çalışmayı bağımsız olarak planlayıp, gerçekleştirerek istatistiksel analizlerini yapar. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Uses the principles of professional development and lifelong learning in their studies. | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | Uses critical thinking, decision making and evidence-based medicine applications in their research. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | By communicating effectively, discusses the issues related to the field and defends their views. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Works with relevant disciplines in the process of selecting, applying and interpreting appropriate methods for studies and establish functional communication. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General information about histological examination method | Self-study, reading recommended soruces | Öğretim Yöntemleri: Anlatım |
| 2 | Microscope types and their usage areas | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 3 | İntended purpose of the solutions used in light microscopic tissue preperation. | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 4 | Light microscopic tissue preparation methods | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 5 | Light microscopic staining methods | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 6 | Basic principles of the Histochemistry | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 7 | Histochemical methods commonly used | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 8 | Mid-Term Exam | Self-study | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | The basic principles of immunohistochemical techniques | Self-study, reading recommended soruces | Öğretim Yöntemleri: Anlatım, Gösteri |
| 10 | Solutions used in immunohistochemical methods | Self-study | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 11 | Immunohistochemical staining steps | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 12 | Electron microscopic tissue preparation methods | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 13 | The solution and dyes used in electron microscopic tissue preperation | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 14 | The basic principles of Immunoelectronmicroscopic method | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 15 | The selection of histological techniques | Self-study | Öğretim Yöntemleri: Anlatım, Gösteri |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 122 | ||
| Total Workload / 25 (h) | 4,88 | ||
| ECTS | 5 ECTS | ||