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Information
| Code | Trans606 |
| Name | Translational Laboratory Techniques II |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 1-4 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | İngilizce |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
To learn the basic applications of molecular biology techniques in the laboratory.
Course Content
Examination of Basic Molecular Techniques in Laboratory Applications
Course Precondition
None
Resources
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler,Nobel Tıp Kitabevleri
Notes
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler,Nobel Tıp Kitabevleri
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Has knowledge of basic molecular biology techniques |
| LO02 | Knows in which researches the basic methods in molecular biology are used |
| LO03 | Knows what laboratory conditions molecular applications require |
| LO04 | Understands the scientific importance of molecular methods |
| LO05 | Knows current molecular approaches and has knowledge about literature review |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops and deepens the current and advanced level knowledge of translational medicine via unique thoughts or researches at a level of proficiency, find out original definitions that will bring innovation in the field of translational medicine. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Conceives the interdisciplinary interactions related to translational medicine; analyzes synthesizes and evaluates original and new thoughts. | 4 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Explains the usage of tool, devices and instruments requiered for knowledge and technologies about translational medicine and its related disciplines. | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Defines frequently used statistical methods in translational medicine and related disciplines, uses statistical softwares effectively. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Uses both theoretical and practical knowledge at an advanced level in the studies related to translational medicine. | |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Develops a new thoughts, method and designment/application which brings innovation in translational medicine or implements a known thoughts, method and designment/application in different fields, investigates, comprehends, designs, adapts, implements an original topic. | |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Writes the report of his/her research which he/she participated, | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Makes necessary investigations by using tool, devices and instrument required for knowledge and technologies about translational medicine and related disciplines at an advanced level, develops a new and creative solution (device, method, treatment, drug) for the problems. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Uses statistical software in the field of Translational Medicine effectively, chooses statistical methods correctly, calculates and interprets correctly. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Maintains the organization of Translational Medicine laboratories and develops solutions in case of encountering unforeseen complex situations during laboratory working hours. | 4 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Conducts scientific studies in translational medicine and related fields independently or as a team member. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Writes the report of his/her research in which he/she participated, publishes in a national or international reputed journal (indexing in SCI, SCI-Expanded, SSCI, or AHCI), and presents it at scientific meetings. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Follows evidence-based practices and conducts research on professional practices that will create evidence in their field. | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Applies the principles of advanced professional development and lifelong learning in the field of Translational Medicine. | 5 |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicate current developments and studies within the field to both professional and non-professional groups systematically using written, oral and visual techniques by supporting with quantitative and qualitative data. | |
| PLO16 | Bilgi - Kuramsal, Olgusal | Learns how to teach. | 4 |
| PLO17 | Belirsiz | Communicate and discuss orally, in written and visually with peers by using a foreign language at least at a level of European Language Portfolio B2 General Level. | |
| PLO18 | Yetkinlikler - Alana Özgü Yetkinlik | Audit the data gathering, interpretation, implementation and announcement stages by taking into consideration the cultural, scientific, and ethic values and uses these issues for social strategy, implementation plans and frame of quality processes. | |
| PLO19 | Yetkinlikler - Alana Özgü Yetkinlik | Knows the importance of ethical principles and ethical committees for the individual and society, acts ethically. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Discovery of DNA and its use in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Discovery of RNA and its use in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Obtaining DNA and RNA from the cell | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Obtaining protein from the cell | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Extraction of DNA and RNA from tissue | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Protein extraction from tissue | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Review of molecular methods in the literature | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 9 | Quantification of DNA and RNA | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | PCR types and applications | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Agarose gel electrophoresis, RFLP | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Eliza varieties and application method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | DNA sequencing methods and next generation sequencing | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Western blot method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Scanning current molecular methods in the literature | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |