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Information
| Code | BİO644 |
| Name | Tissue Culture |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. AYFER PAZARBAŞI |
| Course Instructor |
1 |
Course Goal / Objective
To present and explain the tissue culture with every aspects.
Course Content
Learning and explaining the tissue culture with every aspects. Determine and design the requirements of tissue culture laboratory, beeing able to list and apply all needed techniques and conditions, beeing able to produce the cells and keep them in the term of cryopreservation.
Course Precondition
There is no precondition for this lesson.
Resources
Culture of animal cells: A Manual of Basic Technique, Fifth Edition, by R. Ian Freshney. Copyright 2005, John Wiley&Sons, Inc.
Notes
Methods in Molecular Biology, vol.290: Basic Cell Culture Protocols, Third Edition, Edited by CD Helgason and CL Miller. Humana Press, Inc., Totowa, NJ.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | The student identifies the terminology of tissue culture. |
| LO02 | The student sorts the criteria for tissue culture. |
| LO03 | The student comprehends the effects and results of aseptic conditions and sterilization on tissue culture. |
| LO04 | The student lists the needed equipments for tissue culture laboratory. |
| LO05 | The student could design and describe a tissue culture laboratory. |
| LO06 | The student could outline the needed materials for performing tissue culture. |
| LO07 | The student knows and applies the techniques and conditions relevant to tissue culture. |
| LO08 | The student knows to keep going the tissue culture and keep them in the term of cryopreservation. |
| LO09 | The student distinguishs mitotic cells in tissue culture. |
| LO10 | The student could find the problem and compete with it during tissue culture. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To make and interpret Medical Genetics, Molecular Genetics and Cytogenetic analyzes | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To have information about genetic diseases | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To have knowledge of Molecular Cell Biology | 1 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To apply fluorescence in-situ hybridization (FISH) technique | 1 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To make DNA isolation and PCR of all kinds of tissue | 1 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To make cell culture of all kinds of tissue (blood, amniotic fluid, chorionic villus biopsy) | 4 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To do cytogenetic analysis by recognizing human chromosomes separately | 4 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To make QF-PCR and to interpret the results | 1 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To have the knowledge and skills to teach Medical Biology and Genetics Courses in Medical Faculties | 1 |
| PLO10 | Bilgi - Kuramsal, Olgusal | To write and implement a research project on his/her own | |
| PLO11 | Bilgi - Kuramsal, Olgusal | To write an article by collecting the information obtained at the end of the research | |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | To organize laboratory courses of Medical Biology and Genetics courses at undergraduate level | 1 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | What is Tissue culture | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma, Grup Çalışması |
| 2 | Preparation of Tissue culture Laboratory | Literature rewiev and reading | Öğretim Yöntemleri: Grup Çalışması |
| 3 | Design of Tissue culture Laboratory | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma, Grup Çalışması |
| 4 | Instrumentation used at a tissue culture laboratory | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Grup Çalışması |
| 5 | Safety of the tissue culture laboratory | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | Aseptic technique, preparation and sterilization | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma |
| 7 | Tissue culture flasks | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 8 | Mid-Term Exam | Preparing a presentation | Ölçme Yöntemleri: Ödev, Performans Değerlendirmesi |
| 9 | Tissue culture media | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 10 | Media without sera | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 11 | Determination of Mycoplasma contamination and eradication of Mycoplasma | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 12 | Primary adherent tissue culture and nonadherent cell lines | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Primary tissue culture | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Cell lines | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Cryopreservation | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Preparing multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav, Performans Değerlendirmesi |
| 17 | Term Exams | Preparing multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav, Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 4 | 8 | 32 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 2 | 2 |
| Total Workload (Hour) | 148 | ||
| Total Workload / 25 (h) | 5,92 | ||
| ECTS | 6 ECTS | ||