EFR517

4 ECTS - 2-0 Duration (T+A)- . Semester- 2 National Credit

Information

Code EFR517
Name
Term 2022-2023 Academic Year
Semester . Semester
Duration (T+A) 2-0 (T-A) (17 Week)
ECTS 4 ECTS
National Credit 2 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator


Course Goal / Objective

It is aimed to explain and applications of molecular biological and biochemical methods used in the investigation of subcellular components and signal transduction system in pharmacology.

Course Content

In the protein measurement, protein expression, measurement of enzyme activity, western-blot, ELISA, polymerase chain reactions used in the investigation of subcellular components and signal transducing systems in molecular pharmacology studies, laboratory safety and basic principles in molecular studies

Course Precondition

None

Resources

Molecular Pharmacology: From DNA to Drug Discovery, Willey-Blackwell, 2013 Principles and Technical Aspect of PCR Amplification, Springer, 2008

Notes

Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 1982


Course Learning Outcomes

Order Course Learning Outcomes
LO01 List and apply the rules to be considered in laboratory safety and molecular studies.
LO02 Provide sterilization conditions and safe storage of preparations in molecular studies.
LO03 Describe the current methods used in pharmacological studies.
LO04 Relates and interprets methods that can be used in research of subcellular components and signal transduction system in pharmacology experiments.
LO05 Explain and apply molecular methods used in pharmacology research.
LO06 Explain the principle of Western Blot technique, apply and explain in molecular pharmacology research.
LO07 Explain the principle of reverse transcriptase chain reaction technique, apply and explain in molecular pharmacology research.
LO08 Explain the principle of ELISA technique and its use in molecular pharmacology.
LO09 Explain the principle of immunohistochemistry technique and its use in molecular pharmacology.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Explain the mechanisms of action of drugs, their pharmacokinetic and pharmacodynamic effects. 1
PLO02 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Acts in accordance with ethical and legal principles in the field of pharmacology.
PLO03 Beceriler - Bilişsel, Uygulamalı Can project and conduct research in the field of pharmacology and analyze and interpret data. 3
PLO04 Yetkinlikler - Alana Özgü Yetkinlik It plays an active role in rational drug use and provides counseling to the patient for the correct use of the drug.
PLO05 Yetkinlikler - İletişim ve Sosyal Yetkinlik Can convey the current developments or studies in the field of pharmacology verbally or in writing. 3
PLO06 Bilgi - Kuramsal, Olgusal Follows scientific literature and current developments in the field of pharmacology. 3
PLO07 Bilgi - Kuramsal, Olgusal Establishes a relationship between the effects, mechanisms of action and drug interactions of drugs. 2
PLO08 Bilgi - Kuramsal, Olgusal Explain the physiopathology of various diseases and evalue the drugs used in the treatment of these diseases.
PLO09 Beceriler - Bilişsel, Uygulamalı Knows and uses good laboratory practices. 5
PLO10 Beceriler - Bilişsel, Uygulamalı Uses the theoretical and applied knowledge gained in the field of pharmacology.
PLO11 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Have the ability to take part in in vitro/in vivo animal studies. 3


Week Plan

Week Topic Preparation Methods
1 Introduction The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Beyin Fırtınası, Soru-Cevap, Anlatım
2 Considerations in Laboratory Safety and Molecular Studies The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Soru-Cevap
3 Molecular Pharmacology Studies The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
4 Methods that can be used in Subcellular Components and Signal Transduction System Research in Pharmacology Experiments The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Tartışma
5 Cell culture The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Soru-Cevap
6 Preparation of Homogenate from Tissues The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Gösterip Yaptırma
7 Protein Measurement Methods The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Soru-Cevap
8 Mid-Term Exam The presentation should be prepare from literature and textbook. Ölçme Yöntemleri:
Ödev
9 Basic Principles of Western-Blot Method, Investigation of Protein Expression by Western-Blot The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Soru-Cevap
10 Western-Blot Application and Analysis of Data The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Gösterip Yaptırma
11 mRNA Analysis by Polymerase Chain Reaction and Reverse Transcriptase Polymerase Chain Reaction The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Tartışma, Beyin Fırtınası
12 Basic Principles of Reverse Transcriptase Polymerase Chain Reaction Technique, Construction, Data Analysis The subject should be reviewed from the textbooks Öğretim Yöntemleri:
Anlatım, Soru-Cevap
13 ELISA Method and its use in pharmacological research The subject should be reviewed from the literature Öğretim Yöntemleri:
Anlatım, Soru-Cevap
14 Immunohistochemistry and its use in pharmacological research The subject should be reviewed from the literature Öğretim Yöntemleri:
Anlatım, Soru-Cevap
15 Student's Presentations and Discussion Prepare a presentation Öğretim Yöntemleri:
Tartışma, Soru-Cevap
16 Term Exams Notes should be studied. Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Notes should be studied. Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 2 28
Out of Class Study (Preliminary Work, Practice) 14 2 28
Assesment Related Works
Homeworks, Projects, Others 1 0 0
Mid-term Exams (Written, Oral, etc.) 1 8 8
Final Exam 1 24 24
Total Workload (Hour) 88
Total Workload / 25 (h) 3,52
ECTS 4 ECTS

Update Time: 14.11.2022 10:07