KM334 Biochemistry 2 Lab.

2 ECTS - 0-4 Duration (T+A)- 6. Semester- 2 National Credit

Information

Code KM334
Name Biochemistry 2 Lab.
Term 2023-2024 Academic Year
Semester 6. Semester
Duration (T+A) 0-4 (T-A) (17 Week)
ECTS 2 ECTS
National Credit 2 National Credit
Teaching Language Türkçe
Level Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. RAMAZAN BİLGİN
Course Instructor Prof. Dr. GÜZİDE YÜCEBİLGİÇ (A Group) (Ins. in Charge)
Prof. Dr. RAMAZAN BİLGİN (A Group) (Asst. Ins.)


Course Goal / Objective

Application of biochemical analysis methods, evaluation and interpretation of experimental results with theoretical knowledge.

Course Content

Determination of vitamin C in fruit juice, Determination of urea in serum, Isolation of DNA, Determination of Hemoglobin in whole blood, Polarimeter experiment, Determination of molecular weight of proteins with SDS-PAGE electrophoresis, Lowry protein method, Lowry protein method, Immobilization of lipase onto Celite.

Course Precondition

Yok

Resources

Labaoratory text book

Notes

Labaoratory text book


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Learn biochemical analysis methods
LO02 Evaluate and interpret experimental results
LO03 Learn isolation of DNA
LO04 Learn determination of molecular weight of proteins with SDS-PAGE electrophoresis
LO05 Learn Lowry protein method
LO06 Learn determination of lipase activity


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Relates his/her knowledge in the field of chemistry to life and industry by relying on his/her knowledge 3
PLO02 Bilgi - Kuramsal, Olgusal Evaluates the developments in the world by following and perceiving the orientations in the industry. 4
PLO03 Bilgi - Kuramsal, Olgusal Gains awareness of lifelong learning and renews his/her knowledge. 3
PLO04 Bilgi - Kuramsal, Olgusal Access to information and associate their knowledge with other fields of science. 4
PLO05 Bilgi - Kuramsal, Olgusal Understands the need to combine information in the application of information and perceives the total quality awareness. 3
PLO06 Bilgi - Kuramsal, Olgusal S/he has knowledge in the use of resources, in the creation of technology depending on the country's resources. 3
PLO07 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği S/he conveys what s/he knows to people close or distant to the subject by concretising. 4
PLO08 Yetkinlikler - Öğrenme Yetkinliği Gains the ability to access new information and technology. 5
PLO09 Bilgi - Kuramsal, Olgusal Learns to think creatively and produce solutions to problems in the field. 3
PLO10 Yetkinlikler - Öğrenme Yetkinliği To be able to associate information by having the ability of reasoning and transfer what they know to practice. 5
PLO11 Beceriler - Bilişsel, Uygulamalı Sets up laboratory equipment and makes decisions alone. 3
PLO12 Bilgi - Kuramsal, Olgusal To be able to follow the current technology in the field of chemistry and gain added value. 3
PLO13 Bilgi - Kuramsal, Olgusal Follows the developments, gains the ability to follow the directions in the field. 4
PLO14 Bilgi - Kuramsal, Olgusal Knowing information sharing and participating in teamwork and directing the team. 2
PLO15 Beceriler - Bilişsel, Uygulamalı Gains universal thinking and critical perspective. 4
PLO16 Bilgi - Kuramsal, Olgusal Starts to have a say in the field.


Week Plan

Week Topic Preparation Methods
1 Determination of vitamin C in fruit juice Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
2 Determination of urea in serum Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
3 Isolation of DNA Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
4 Determination of Hemoglobin in whole blood Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
5 Polarimeter experiment Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
6 Determination of molecular weight of proteins with SDS-PAGE electrophoresis Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
7 Determination of molecular weight of proteins with SDS-PAGE electrophoresis I Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
8 Mid-Term Exam Study related lecture notes Ölçme Yöntemleri:
Yazılı Sınav
9 Lowry protein method Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
10 Determination of lipase activity by titration Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
11 Immobilization of lipase onto Celite Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
12 Immobilization of lipase onto Celite I Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
13 Immobilization of lipase onto Celite II Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
14 Make-up experiments Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
15 Make-up experiments I Biochemistry books, lecture notes Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
16 Term Exams Study related lecture notes Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Study related lecture notes Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 1 14
Out of Class Study (Preliminary Work, Practice) 14 2 28
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 4 4
Final Exam 1 12 12
Total Workload (Hour) 58
Total Workload / 25 (h) 2,32
ECTS 2 ECTS

Update Time: 05.05.2023 10:42