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Information
| Code | BFZ504 |
| Name | Intracellular Potential Measurement Techniques |
| Term | 2023-2024 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MUSTAFA EMRE |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to introduce intracellular potential measurement techniques used in biophysics.
Course Content
Membrane current / transmittance components, voltage clamping and patch clamping recording technique used in single channel investigations will be explained.
Course Precondition
There is no prerequisite for the course.
Resources
Related articles
Notes
Internet resources
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns the concept of membrane potential and its properties. |
| LO02 | Learns the electrical properties of nerve cells. |
| LO03 | Learns basic electrophysiology information |
| LO04 | Knows voltage clamp theory and practice knowledge. |
| LO05 | Knows patch clamp theory and practice knowledge. |
| LO06 | Learns the analysis of electrophysiology recording data. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Learns basic information about the field | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Sets up and works experimental setups in the laboratory | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Prepares a project proposal on its own | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Plans an experimental study | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Reports the research result | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Interpret the research findings | 1 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Has the analytical thinking mechanism related to the field, Reaches reliable information. Creates and writes a research project, works and researches based on ethical values. | |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | She/he systematically transfers the theoretical knowledge she has researched to the audience. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Have a scientific thinking systematic in relation to their profession. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to membrane potentials 1 | Reading related text book | Öğretim Yöntemleri: Anlatım, Tartışma, Deney / Laboratuvar |
| 2 | Introduction to membrane potentials 2 | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 3 | Kinetic analysis of membrane currents | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 4 | passive properties of nerve cells | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 5 | Basic electrophysiology methods 1 | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 6 | Basic electrophysiology methods 2 | Reading related text book | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 7 | Theory of voltage clamp | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Basic voltage clamp protocols 1 | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 10 | Basic voltage clamp protocols 2 | Reading related text book | Öğretim Yöntemleri: Gösterip Yaptırma |
| 11 | Basic patch clamp protocols 1 | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 12 | Basic patch clamp protocols 2 | Reading related text book | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 13 | Evaluation of single channel recordings | Reading related text book | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 14 | Filtering | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 15 | Signal recording processing principles | Reading related text book | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | works related texts | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | works related texts | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 108 | ||
| Total Workload / 25 (h) | 4,32 | ||
| ECTS | 4 ECTS | ||