BK027 Identification of Fungal Plant Diseases With Classic and Molecular Methods

6 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit

Information

Code BK027
Name Identification of Fungal Plant Diseases With Classic and Molecular Methods
Term 2023-2024 Academic Year
Semester . Semester
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Doç. Dr. DAVUT SONER AKGÜL


Course Goal / Objective

Students learn to study and identify of fungal pathogens in laboratory

Course Content

Classical and molecular biology methods in fungal diagnosis

Course Precondition

No pre-requisite

Resources

Plant Pathologist's Pocketbook

Notes

Videos


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Students learn the rules of working in the laboratory
LO02 Learn the principles of sterile working in the laboratory
LO03 Learn to use laboratory devices
LO04 Learn how to make growth media, sterilization and pouring
LO05 Learn to use microscope and examine of fungal objects
LO06 Learn the pathogen isolation from the infected tissues
LO07 Learn pathogen purification and archiving
LO08 Learn how to store fungal cultures
LO09 Learn how to prepare reagents for molecular biology experiments
LO10 Learn DNA extraction
LO11 Learn PCR experiments
LO12 Learn gel electrophoresis experiments
LO13 Learn BLAST analysis
LO14 Learn to make and interpret phylogenetic tree
LO15 Learn how to register a sequence and get accession number


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Has knowledge on basic topics about general science, general agricultural engineering and plant production
PLO02 Yetkinlikler - Öğrenme Yetkinliği Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants
PLO03 Yetkinlikler - Öğrenme Yetkinliği Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice
PLO04 Yetkinlikler - Öğrenme Yetkinliği Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods
PLO05 Beceriler - Bilişsel, Uygulamalı Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports 1
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly
PLO07 Yetkinlikler - Öğrenme Yetkinliği Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations
PLO08 Bilgi - Kuramsal, Olgusal By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education
PLO09 Yetkinlikler - Alana Özgü Yetkinlik He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in.
PLO10 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Can present results of the scientific research as a report/thesis and produce scientific publications
PLO11 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production.
PLO12 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems.
PLO13 Yetkinlikler - Öğrenme Yetkinliği Develops skills in using laboratory methods and techniques 4
PLO14 Yetkinlikler - Alana Özgü Yetkinlik Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection.


Week Plan

Week Topic Preparation Methods
1 Principles working in laboratory Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
2 How to make laboratory devices Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
3 Use of pH meter and preparation of medium Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
4 Microscope use Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
5 Pathogen isolation from plant tissues Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
6 Subculturing and single spor isolation Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
7 Storage and archiving of fungal cultures Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
8 Mid-Term Exam Doing Experiment Ölçme Yöntemleri:
Performans Değerlendirmesi
9 Preparing solutions for molecular biology experiments Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
10 DNA extraction Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
11 PCR experiments Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
12 Gel electrophoresis Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
13 Gen sequencing and BLAST analysis Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
14 Making phylogenetic tree Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
15 Getting an accession number from GenBank Books and publications Öğretim Yöntemleri:
Gösterip Yaptırma
16 Term Exams Doing Experiment Ölçme Yöntemleri:
Performans Değerlendirmesi
17 Term Exams Applied exam Ölçme Yöntemleri:
Performans Değerlendirmesi


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 5 70
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 15 15
Final Exam 1 30 30
Total Workload (Hour) 157
Total Workload / 25 (h) 6,28
ECTS 6 ECTS

Update Time: 13.09.2023 11:33