Information
Code | BK027 |
Name | Identification of Fungal Plant Diseases With Classic and Molecular Methods |
Term | 2023-2024 Academic Year |
Semester | . Semester |
Duration (T+A) | 3-0 (T-A) (17 Week) |
ECTS | 6 ECTS |
National Credit | 3 National Credit |
Teaching Language | Türkçe |
Level | Yüksek Lisans Dersi |
Type | Normal |
Mode of study | Yüz Yüze Öğretim |
Catalog Information Coordinator | Doç. Dr. DAVUT SONER AKGÜL |
Course Goal / Objective
Students learn to study and identify of fungal pathogens in laboratory
Course Content
Classical and molecular biology methods in fungal diagnosis
Course Precondition
No pre-requisite
Resources
Plant Pathologist's Pocketbook
Notes
Videos
Course Learning Outcomes
Order | Course Learning Outcomes |
---|---|
LO01 | Students learn the rules of working in the laboratory |
LO02 | Learn the principles of sterile working in the laboratory |
LO03 | Learn to use laboratory devices |
LO04 | Learn how to make growth media, sterilization and pouring |
LO05 | Learn to use microscope and examine of fungal objects |
LO06 | Learn the pathogen isolation from the infected tissues |
LO07 | Learn pathogen purification and archiving |
LO08 | Learn how to store fungal cultures |
LO09 | Learn how to prepare reagents for molecular biology experiments |
LO10 | Learn DNA extraction |
LO11 | Learn PCR experiments |
LO12 | Learn gel electrophoresis experiments |
LO13 | Learn BLAST analysis |
LO14 | Learn to make and interpret phylogenetic tree |
LO15 | Learn how to register a sequence and get accession number |
Relation with Program Learning Outcome
Order | Type | Program Learning Outcomes | Level |
---|---|---|---|
PLO01 | Bilgi - Kuramsal, Olgusal | Has knowledge on basic topics about general science, general agricultural engineering and plant production | |
PLO02 | Yetkinlikler - Öğrenme Yetkinliği | Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants | |
PLO03 | Yetkinlikler - Öğrenme Yetkinliği | Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice | |
PLO04 | Yetkinlikler - Öğrenme Yetkinliği | Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods | |
PLO05 | Beceriler - Bilişsel, Uygulamalı | Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports | 1 |
PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly | |
PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations | |
PLO08 | Bilgi - Kuramsal, Olgusal | By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education | |
PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in. | |
PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Can present results of the scientific research as a report/thesis and produce scientific publications | |
PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production. | |
PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems. | |
PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develops skills in using laboratory methods and techniques | 4 |
PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection. |
Week Plan
Week | Topic | Preparation | Methods |
---|---|---|---|
1 | Principles working in laboratory | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
2 | How to make laboratory devices | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
3 | Use of pH meter and preparation of medium | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
4 | Microscope use | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
5 | Pathogen isolation from plant tissues | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
6 | Subculturing and single spor isolation | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
7 | Storage and archiving of fungal cultures | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
8 | Mid-Term Exam | Doing Experiment | Ölçme Yöntemleri: Performans Değerlendirmesi |
9 | Preparing solutions for molecular biology experiments | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
10 | DNA extraction | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
11 | PCR experiments | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
12 | Gel electrophoresis | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
13 | Gen sequencing and BLAST analysis | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
14 | Making phylogenetic tree | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
15 | Getting an accession number from GenBank | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
16 | Term Exams | Doing Experiment | Ölçme Yöntemleri: Performans Değerlendirmesi |
17 | Term Exams | Applied exam | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
Works | Number | Time (Hour) | Workload (Hour) |
---|---|---|---|
Course Related Works | |||
Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
Assesment Related Works | |||
Homeworks, Projects, Others | 0 | 0 | 0 |
Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
Final Exam | 1 | 30 | 30 |
Total Workload (Hour) | 157 | ||
Total Workload / 25 (h) | 6,28 | ||
ECTS | 6 ECTS |