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Information
| Code | BK027 |
| Name | Identification of Fungal Plant Diseases With Classic and Molecular Methods |
| Term | 2023-2024 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. DAVUT SONER AKGÜL |
| Course Instructor |
1 |
Course Goal / Objective
Students learn to study and identify of fungal pathogens in laboratory
Course Content
Classical and molecular biology methods in fungal diagnosis
Course Precondition
No pre-requisite
Resources
Plant Pathologist's Pocketbook
Notes
Videos
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Students learn the rules of working in the laboratory |
| LO02 | Learn the principles of sterile working in the laboratory |
| LO03 | Learn to use laboratory devices |
| LO04 | Learn how to make growth media, sterilization and pouring |
| LO05 | Learn to use microscope and examine of fungal objects |
| LO06 | Learn the pathogen isolation from the infected tissues |
| LO07 | Learn pathogen purification and archiving |
| LO08 | Learn how to store fungal cultures |
| LO09 | Learn how to prepare reagents for molecular biology experiments |
| LO10 | Learn DNA extraction |
| LO11 | Learn PCR experiments |
| LO12 | Learn gel electrophoresis experiments |
| LO13 | Learn BLAST analysis |
| LO14 | Learn to make and interpret phylogenetic tree |
| LO15 | Learn how to register a sequence and get accession number |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Has knowledge on basic topics about general science, general agricultural engineering and plant production | |
| PLO02 | Yetkinlikler - Öğrenme Yetkinliği | Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants | |
| PLO03 | Yetkinlikler - Öğrenme Yetkinliği | Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice | |
| PLO04 | Yetkinlikler - Öğrenme Yetkinliği | Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports | 1 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations | |
| PLO08 | Bilgi - Kuramsal, Olgusal | By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Can present results of the scientific research as a report/thesis and produce scientific publications | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develops skills in using laboratory methods and techniques | 4 |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Principles working in laboratory | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 2 | How to make laboratory devices | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 3 | Use of pH meter and preparation of medium | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 4 | Microscope use | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 5 | Pathogen isolation from plant tissues | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 6 | Subculturing and single spor isolation | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 7 | Storage and archiving of fungal cultures | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 8 | Mid-Term Exam | Doing Experiment | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 9 | Preparing solutions for molecular biology experiments | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 10 | DNA extraction | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 11 | PCR experiments | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 12 | Gel electrophoresis | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 13 | Gen sequencing and BLAST analysis | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 14 | Making phylogenetic tree | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 15 | Getting an accession number from GenBank | Books and publications | Öğretim Yöntemleri: Gösterip Yaptırma |
| 16 | Term Exams | Doing Experiment | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 17 | Term Exams | Applied exam | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||