ZO027 Gene Cloning

6 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit
Information About Course

Information
Goal, Content & Resources
Course Learning Outcomes
Relation with Program Learning Outcome
Week Plan
Student Workload - ECTS

Information

Code ZO027
Name Gene Cloning
Term 2023-2024 Academic Year
Term Fall
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Doktora Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. BAHRİ DEVRİM ÖZCAN
Course Instructor
1


Course Goal / Objective

To teach the importance of gene cloning, methods, agricultural and industrial applications to the students.

Course Content

The importance and purpose of gene cloning in prokaryotic organisms, DNA isolation from cell, identification of the gene to be cloned, identification of the organism for gene transfer, viruses, phages, plasmids, DNA transformation into the viable cell, determination of cloned gene in the target cell, purification of the clone, practical applications.

Course Precondition

No prerequisite

Resources

Gene Cloning and DNA Analysis: An Introduction, 8th Edition, T. A. Brown, Wiley-Blackwell, ISBN: 978-1-119-64078-3

Notes

Moleküler Biyoteknoloji, Rekombinant DNA'nın Prensipleri ve Uygulamaları, B.R. Glick, C.L. Patten, Beşinci Baskıdan Çeviri, 2021. Çeviri Editörü: T. Kankılıç, Palme Yayınları, Ankara


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Know gene cloning techniques in microorganisms
LO02 Isolate the DNA and gene from organizms
LO03 Knows cloning vectors, their role in cloning and how to use them
LO04 Understands the biotechnological, agricultural and industrial importance of gene cloning in microorganisms
LO05 Produce technological solutions to problems related to the field


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal After undergraduate education, increases knowledge in one of the fields of animal breeding and breeding, feeds and animal nutrition, biometrics and genetics. 2
PLO02 Bilgi - Kuramsal, Olgusal Understands the interaction between different disciplines
PLO03 Bilgi - Kuramsal, Olgusal Gains the ability to develop strategic approaches and produce regional, national or international solutions for the field of animal science 1
PLO04 Bilgi - Kuramsal, Olgusal Gains the ability to develop knowledge with scientific methods by using the data in animal science, and to use this knowledge with the awareness of scientific, social and ethical responsibility.
PLO05 Bilgi - Kuramsal, Olgusal Gains the ability to use and develop information technologies with computer software and hardware knowledge required by the field of animal science.
PLO06 Bilgi - Kuramsal, Olgusal Gains the ability to convey their own studies or current developments in the field of animal science to groups in the field or other fields of science, verbally and visually.
PLO07 Bilgi - Kuramsal, Olgusal Gains the ability to evaluate the quality processes of animal products
PLO08 Bilgi - Kuramsal, Olgusal Gains the ability to keep animal production dynamic in accordance with changing economic and social conditions. 2
PLO09 Bilgi - Kuramsal, Olgusal Gains the ability to follow national and international current issues, to follow developments in lifelong learning, science and technology, to constantly renew themselves and to transfer innovations to animal production. 3
PLO10 Bilgi - Kuramsal, Olgusal Hayvansal ürünler ile insan sağlığı ve toplum refahı arasındaki ilişkiyi özümser


Week Plan

Week Topic Preparation Methods
1 Importance of gene cloning 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
2 Cloning vectors 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
3 Isolation of chromosomal and plasmid DNA from microorganisms 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
4 DNA modifying enzymes 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
5 Restriction endonucleases 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
6 Compatent cell preparation 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
7 Preparation to mid-term exam Preparation to mid-term exam
8 Mid-Term Exam Mid-term exam
9 Shotgun cloning 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
10 PCR-based cloning 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
11 Gene transfer methods in microorganisms 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
12 Selection and analysis of recombinant bacteria 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
13 Biotechnologic importance of gene cloning 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
14 Agricultural importance of gene cloning 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
15 Industrial importance of gene cloning 1) Molecular Genetics of Bacteria, Edited by Jeremy W. Dale, 3th edition, John Wiley and Sons, 1999. 2) Gen Klonlama ve DNA Analizi Giriş, T.A. Brown, 5. baskıdan çeviri, Çeviri Kurulu: Bardakcı, F., Yenidünya, A.F., Yılmaz, N., Nobel Yayınları, 2009.
16 Term Exams Term exam
17 Term Exams Term exam


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 5 70
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 15 15
Final Exam 1 30 30
Total Workload (Hour) 157
Total Workload / 25 (h) 6,28
ECTS 6 ECTS

Update Time: 10.05.2023 03:00