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Information
| Code | TBB516 |
| Name | Bacterial Isolation and Inoculation Methods |
| Term | 2023-2024 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-1 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 2.5 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MUSTAFA GÖK |
| Course Instructor |
1 |
Course Goal / Objective
To provide students with knowledge and experience in isolation and inoculation of various bacteria living in soil and legume nodules.
Course Content
General and specific media, culture sampling, dilution and inoculation techniques, obtaining pure culture, morphological and microscopic observations, Gram-staining, O / F, oxidase, catalase, urease tests, classification.
Course Precondition
No prerequisites
Resources
Course notes are shared with students through the system.
Notes
Current national-international articles on course content.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Recognize microbial groups in soil. |
| LO02 | Learn the reproduction of microorganisms and the laboratory methods. |
| LO03 | Understands the importance of microbial isolation methods. |
| LO04 | Learns microbial inoculation. |
| LO05 | Learns the importance of inoculation in plant nutrition and fertilization. |
| LO06 | Understands the importance of microbial fertilization in plant nutrition. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Has the intellectual knowledge and skills about the classification of soils in accordance with natural and technical soil classification systems in accordance with their abilities and principles of sustainability by applying the theoretical and practical knowledge in different disciplines and other engineering fields related to his / her field and apply them | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | Uses the intellectual knowledge to apply new techniques on plant nutrients and applications that provide right nutrition of plants for high quality and economic, environmental and socially sustainable crop production by applying scientific approaches | 3 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Develops a new method or applies a known method in different areas that can solve existing problems in dimensions that include the other disciplines in order to analyze soils, their components or the process in which the soils are in and to fulfill the requirements of the principles of sustainable agriculture | 3 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Gains the ability to conduct research, design and apply the project in a way to include other disciplines in order to solve the problems encountered in ensuring the proper nutrition of plants in soil or soilless environment | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Gains competence in designing, researching and adapting innovations in the application, adaptation and modification of all kinds of soil, plant, fertilizer and water analysis | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Contributes to science through an original study that can be published by analyzing problems related to her/his profession by developing a new scientific method or applying a known method | |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes the leadership position in cases where specific and interdisciplinary problems need to be solved | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods in the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making; develops a positive attitude towards lifelong learning with the knowledge and skills acquired | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Has the ability to communicate effectively in front of an expert group in defending his original ideas, using English (at C1 level) as well besides Turkish | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Examines social relations and the norms that direct these relations from a critical point of view, and manages the actions directed to change in order to improve them | |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Contributes to the process of becoming an information society by introducing the technological advancements in the field in academic and professional context | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | Produces solutions for the social, scientific, and ethical problems in the field and in business life and contributes to the development of these values | |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | While specializing in special issues in her/his discipline, she/he establishes functional interaction by using strategic decision-making processes in solving the problems encountered. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Types of media | Laboratory preparation | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Culture sampling | Laboratory preparation | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Dilution | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Vaccination techniques | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma, Deney / Laboratuvar |
| 5 | Obtaining pure culture | Laboratory preparation | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Colony recognition | Laboratory preparation | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Microbial counting methods | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 8 | Mid-Term Exam | Mid-term exam preparation | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
| 9 | Morphological-microscopic observations | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Gram painting | Review of the topic | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | O/F test | Review of the topic | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 12 | Biochemical analyzes - oxidase | Review of the topic | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 13 | Catalase test | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma, Deney / Laboratuvar |
| 14 | Urease test | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma, Deney / Laboratuvar |
| 15 | Bacterial classification. | Review of the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 16 | Term Exams | Revision of the topics expressed before, Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Revision of the topics expressed before, Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 116 | ||
| Total Workload / 25 (h) | 4,64 | ||
| ECTS | 5 ECTS | ||