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Information
| Code | DM009 |
| Name | Animal Cell Culture Applications for Tissue Engineering I |
| Term | 2023-2024 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
Learning the Application Areas of Basic Methods in Cell Culture
Course Content
Cell culture Techniques, Devices Used in Cell Culture and Their Properties, Cell Culture Sowing, Propagation, Passaging and Freezing Methods, Cell Culture Safety Conditions
Course Precondition
none
Resources
Lecture Notes
Notes
Freshney RI., Culture of Animas Cells:A Manual of Basic Technique
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Can choose a medium to support cell culture |
| LO02 | Has knowledge of the basic features of cell culture |
| LO03 | Knows the differences of cell culture types and has information about comparing cell structures |
| LO04 | Has the knowledge to choose the appropriate medium for the structure of the cells according to their types |
| LO05 | Define the coordination of medium and support medium in cell culture |
| LO06 | Can identify contaminations that may occur in cell culture |
| LO07 | Determine what contamination may be in cell culture and evaluate the appropriate solution |
| LO08 | Has knowledge of the safety of the person working in cell culture applications |
| LO09 | Define the methods that can be used in living cell determination |
| LO10 | Has knowledge about the methods of using cells obtained from cell culture in molecular studies |
| LO11 | Has knowledge about the cryopreservation and storage conditions of sub-passages in cell culture |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Having advanced knowledge of the concepts, theories and principles of the Tissue Engineering Department | 5 |
| PLO02 | Yetkinlikler - Alana Özgü Yetkinlik | Being equipped with advanced mathematics, science and engineering knowledge | |
| PLO03 | Yetkinlikler - Alana Özgü Yetkinlik | To be able to design and perform advanced experiments in the Tissue Engineering Department; To be able to analyze and interpret the results and the data obtained. | 4 |
| PLO04 | Yetkinlikler - Alana Özgü Yetkinlik | Be equipped with a variety of advanced engineering techniques and skills | |
| PLO05 | Yetkinlikler - Alana Özgü Yetkinlik | Ability to lead the definition, design, development and deployment of a product or production method | 3 |
| PLO06 | Yetkinlikler - Alana Özgü Yetkinlik | Being aware of the necessity of lifelong learning and being able to constantly renew oneself | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | Ability to use English effectively both verbally and in writing on technical or non-technical subjects | 5 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Being prone to teamwork, being able to communicate well with the environment; Ability to work efficiently as a member of a multi-faceted and interdisciplinary team | |
| PLO09 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Being curious and innovative, aware of its technical and ethical responsibilities | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Gains high-level skills in using research methods in studies related to the field of Tissue Engineering. | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | What are the main cell culture application areas? | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Cell culture types | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Tecniques used in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Cell culture media | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Support media in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Cell culture contaminations | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Measures to be taken against cell culture contamination | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 9 | Safety conditions in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Cell viability determination methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Molecular methods that can be used after cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Cell culture freezing methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Storage conditions of frozen cells | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Cell count | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Flask selection according to application method in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||