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Information
| Code | Trans602 |
| Name | DNA Damage and Repair |
| Term | 2023-2024 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | İngilizce |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
The learning objective here is for students to gain an understanding of the major DNA repair pathways in eukaryotic cells, highlighting proteins involved in the cellular responses to ionizing radiation or anti-cancer chemotherapy. We will cover the main DNA repair pathways including base excision repair, mismatch repair, nucleotide excision repair, and DNA single strand and double strand break repair. We will stress the mechanisms of non-homologous end joining and homologous recombination repair. The topics of ATM and ATR dependent signaling, H2AX foci and roles of protein phosphorylation and protein ubiquitination, and the relationship of replication stress to the DNA damage response will be covered, as well has human diseases of genomic instability and cancer predisposition.
Course Content
DNA repair pathways, highlighting the cellular responses to ionizing radiation will be covered, including: base excision repair, mismatch repair, nucleotide excision repair, and DNA single strand and double strand break repair. The relationship of replication stress to genomic instability in a cancer context will also be covered, as well has human diseases of genomic instability and cancer predisposition.
Course Precondition
Masters level Molecular Biology knowledge is required
Resources
Academic search tools and presentation documents.
Notes
https://pubmed.ncbi.nlm.nih.gov/?term=DNA+damage+and+repair&filter=pubt.review&size=100
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | The learning objective here is for students to gain an understanding of the major DNA repair pathways in eukaryotic cells, highlighting proteins involved in the cellular responses to ionizing radiation or anti-cancer chemotherapy |
| LO02 | The students will understand the relationship of replication stress to the DNA damage response will be covered, as well has human diseases of genomic instability and cancer predisposition |
| LO03 | Students read and understand article related to DNA damage and Repair system |
| LO04 | They learn the techniques to study DNA damage and Repair pathways |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops and deepens the current and advanced level knowledge of translational medicine via unique thoughts or researches at a level of proficiency, find out original definitions that will bring innovation in the field of translational medicine. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Conceives the interdisciplinary interactions related to translational medicine; analyzes synthesizes and evaluates original and new thoughts. | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Explains the usage of tool, devices and instruments requiered for knowledge and technologies about translational medicine and its related disciplines. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Defines frequently used statistical methods in translational medicine and related disciplines, uses statistical softwares effectively. | 2 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Uses both theoretical and practical knowledge at an advanced level in the studies related to translational medicine. | 2 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Develops a new thoughts, method and designment/application which brings innovation in translational medicine or implements a known thoughts, method and designment/application in different fields, investigates, comprehends, designs, adapts, implements an original topic. | 3 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Writes the report of his/her research which he/she participated, | 4 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Makes necessary investigations by using tool, devices and instrument required for knowledge and technologies about translational medicine and related disciplines at an advanced level, develops a new and creative solution (device, method, treatment, drug) for the problems. | 3 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Uses statistical software in the field of Translational Medicine effectively, chooses statistical methods correctly, calculates and interprets correctly. | 1 |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Maintains the organization of Translational Medicine laboratories and develops solutions in case of encountering unforeseen complex situations during laboratory working hours. | 1 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Conducts scientific studies in translational medicine and related fields independently or as a team member. | 4 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Writes the report of his/her research in which he/she participated, publishes in a national or international reputed journal (indexing in SCI, SCI-Expanded, SSCI, or AHCI), and presents it at scientific meetings. | 2 |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Follows evidence-based practices and conducts research on professional practices that will create evidence in their field. | 5 |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Applies the principles of advanced professional development and lifelong learning in the field of Translational Medicine. | 3 |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicate current developments and studies within the field to both professional and non-professional groups systematically using written, oral and visual techniques by supporting with quantitative and qualitative data. | 4 |
| PLO16 | Bilgi - Kuramsal, Olgusal | Learns how to teach a concept, subject, phenomenon or case related to Translational Medicine. | 3 |
| PLO17 | Belirsiz | Communicate and discuss orally, in written and visually with peers by using a foreign language at least at a level of European Language Portfolio B2 General Level. | 5 |
| PLO18 | Yetkinlikler - Alana Özgü Yetkinlik | Audit the data gathering, interpretation, implementation and announcement stages by taking into consideration the cultural, scientific, and ethic values and uses these issues for social strategy, implementation plans and frame of quality processes. | 1 |
| PLO19 | Yetkinlikler - Alana Özgü Yetkinlik | Knows the importance of ethical principles and ethical committees for the individual and society, acts ethically. | 5 |
| PLO20 | Yetkinlikler - Alana Özgü Yetkinlik | Increases the awareness of society about translational medicine. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | The importance of DNA Damage and the repair system | Masters level knowledge of Molecular Biology and Genetics | Öğretim Yöntemleri: Soru-Cevap |
| 2 | Basics I: Base and Nucleotide Damage, DNA Mismatches and pathways of repair | Masters level knowledge of Molecular Biology and Genetics | Öğretim Yöntemleri: Soru-Cevap |
| 3 | Basics I-2: BER and NER | Masters level knowledge of Molecular Biology and Genetics | Öğretim Yöntemleri: Soru-Cevap |
| 4 | Case I Parts 1-3 | Masters level knowledge of Molecular Biology and Genetics | Öğretim Yöntemleri: Örnek Olay |
| 5 | Reviewing Case I parts 1-3 | Masters level knowledge of Molecular Biology and Genetics | Öğretim Yöntemleri: Tartışma |
| 6 | Case I Parts 4-6 | Students will study their cases | Öğretim Yöntemleri: Örnek Olay |
| 7 | Reviewing Case I Parts 4-6 | Students will study their cases | Öğretim Yöntemleri: Tartışma |
| 8 | Mid-Term Exam | Student will prepare homework | Ölçme Yöntemleri: Ödev |
| 9 | DNA double strand breaks, intra/interstrand crosslinks and pathways of repair | Articles to read | Öğretim Yöntemleri: Soru-Cevap |
| 10 | Student presentations 1 “The checkpoint control of cell cycle” “p53 regulation and cell cycle” | Students will study their articles | Öğretim Yöntemleri: Grup Çalışması |
| 11 | Structural Approaches to understand DNA damage repair | Articles to read | Öğretim Yöntemleri: Anlatım |
| 12 | Student presentations 2 “Consequences of BRCA 1 and 2 mutations” “FANCs (A B and C) and Fanconi Anemia” | Articles to read | Öğretim Yöntemleri: Grup Çalışması |
| 13 | Article criticism -1 | Articles to read | Öğretim Yöntemleri: Tartışma |
| 14 | Article criticism-2 | Articles to read | Öğretim Yöntemleri: Tartışma |
| 15 | Final homework assignments | literature review | Öğretim Yöntemleri: Tartışma |
| 16 | Term Exams | Independent study | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Independent study | Ölçme Yöntemleri: Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 114 | ||
| Total Workload / 25 (h) | 4,56 | ||
| ECTS | 5 ECTS | ||