BFZ612 Microscopy and Fluorescent Imaging of the Cell

7 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit
Information About Course

Information
Goal, Content & Resources
Course Learning Outcomes
Relation with Program Learning Outcome
Week Plan
Student Workload - ECTS

Information

Code BFZ612
Name Microscopy and Fluorescent Imaging of the Cell
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 7 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Doktora Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Doç. Dr. FİGEN ÇİÇEK


Course Goal

Learning basic principles of microscopes and decide and choose a proper microscope for a scientific project and use a microscope. Learning fluorescence imaging techniques in the cell and ability to do fluorescence imaging .

Course Content

Basic optic principles, types of microscopes and their working principles. Fluorescent principles and its use in cellular imaging.

Course Precondition

The lesson has no prerequisites

Resources

Lecture notes (For Medical Faculty Students - İsmail Günay) Biophysics (Ferit Pehlivan) Biophysics (Gürbüz Çelebi)

Notes

Internet Research


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Learns basics of light and optics.
LO02 learns the basic principles of image formation in the Microscope
LO03 Learns the selection criteria of the appropriate microscope for the experiments.
LO04 Learns to use a microscope in the laboratory and image data analysis.
LO05 Learns fluorescence with its physics principles
LO06 Learns different fluorescence imaging techniques
LO07 . Learns to decide florophore, type of the florescent system, wavelength and filters according to the experiment.
LO08 Learns image data analysis.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal To learn the basic information about the field 3
PLO02 Bilgi - Kuramsal, Olgusal To be able to establish experimental work in laboratory
PLO03 Bilgi - Kuramsal, Olgusal To prepare a project proposal by itself 2
PLO04 Bilgi - Kuramsal, Olgusal To plan an experimental research
PLO05 Bilgi - Kuramsal, Olgusal To make a report as a result of the research 3
PLO06 Bilgi - Kuramsal, Olgusal To be able to interpret research findings 3
PLO07 Bilgi - Kuramsal, Olgusal Having analytical thinking mechanism related to the field Accessing reliable information Creating and writing a research project Study and research based on ethic values 2
PLO08 Bilgi - Kuramsal, Olgusal Ability to transfer the theoretical knowledge to the audience in a systematic way 2
PLO09 Bilgi - Kuramsal, Olgusal To have scientific thought systematic about the profession 4
PLO10 Bilgi - Kuramsal, Olgusal Kendi başına bir proje önerisi hazırlayabilmek 3


Week Plan

Week Topic Preparation Methods
1 Light and alectromagnetic spectrum Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
2 Basic optic principles 1Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
3 Interference, diffraction and resolving power, polarization 2Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
4 Optical components of the simple microscopes. Magnification and numerical aperture 3Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Tartışma, Anlatım, Alıştırma ve Uygulama
5 Lighting techniques in the microscopes (1); bright field, dark field, phase contrast, cross polarized light 4Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Tartışma, Alıştırma ve Uygulama
6 Lighting techniques in the microscopes (2); Microspectroscopy (UV and UV-vis) epifluorescence microscopes, confocals 5Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Gösterip Yaptırma
7 Other microscope types; Atomic Force, X Ray microscopy, electron microscopy 6Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Alıştırma ve Uygulama, Soru-Cevap, Anlatım
8 Mid-Term Exam Mid-Term Exam Ölçme Yöntemleri:
Yazılı Sınav, Ödev, Performans Değerlendirmesi
9 Basics of the Fluorescence 7Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Tartışma, Gösteri
10 Fluorescence probes and their cellular usages 8Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Soru-Cevap, Anlatım
11 Fluorescent measurement systems and their working principles; Fluorimeter, Camera and confocal systems 9Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Soru-Cevap, Tartışma, Anlatım
12 Ion imaging in the cell and calibration 10Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Soru-Cevap, Alıştırma ve Uygulama, Anlatım
13 1Ion imaging in the cell and calibration 11Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Tartışma, Alıştırma ve Uygulama, Anlatım
14 Measurement of dynamic ion concentrations in the cell 12Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Tartışma, Soru-Cevap, Alıştırma ve Uygulama
15 Fluorescent meaurement of DNA and immunofluorescent techniques 13Reading the related chapter from the textbooks and notes Öğretim Yöntemleri:
Soru-Cevap, Anlatım
16 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav, Ödev, Performans Değerlendirmesi
17 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav, Ödev, Performans Değerlendirmesi


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 6 84
Assesment Related Works
Homeworks, Projects, Others 1 9 9
Mid-term Exams (Written, Oral, etc.) 1 8 8
Final Exam 1 16 16
Total Workload (Hour) 173
Total Workload / 25 (h) 6,92
ECTS 7 ECTS