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Information
| Code | TBP316 |
| Name | Tissue Culture Techniques |
| Term | 2024-2025 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. RÜŞTÜ HATİPOĞLU |
| Course Instructor |
1 2 |
Course Goal / Objective
The students learn the basic principles of tissue culture and the tissue culture techniques used in the plants
Course Content
Definition of tissue culture, organization of tissue culture laboratory, preparation of medium and stelization techniques, embryogenesis. organogenesis, tissue cultue techniques used in the plants
Course Precondition
Non
Resources
Hatipoğlu, R. 2010. Lecture notes of Plant tissue culture techniques
Notes
Özcan, S., Gürel, E. ve Babaoğlu, M. 2001. Plant Biotechnology. Vol.I. Selcuk University Foundation Publications
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns tools and equipment required in the tissue culture laboratory |
| LO02 | Learns the principles of sterile working |
| LO03 | Learns medium preparation for tissue culture |
| LO04 | Learns isolation and culture of plant explant |
| LO05 | Learns processes of tissue culture techniques |
| LO06 | Identify various sustainability practices. |
| LO07 | To learn how to determine economic threshold level of weeds. |
| LO08 | Economic threshold level of important weeds |
| LO09 | To learn biological control of weeds |
| LO10 | To learn physical and mechanical control of weeds |
| LO11 | To learn chemical control of weeds |
| LO12 | To know herbicides and their mode of action |
| LO13 | To learn herbicides and resistance |
| LO14 | To learn rules of herbicides use and protection of environment. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have sufficient theoretical knowledge in the fields of Mathematics, Basic Science and Basic Engineering and Agricultural Engineering. | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | Recognizes the basic field of Field Crops and also cereals, grain legumes, industrial plants, meadow pasture forage crops and energy crops, has information on the problems and solutions in the physiology, cultivation, biotechnological studies, organic farming, breeding and new variety development of these plants. | |
| PLO03 | Yetkinlikler - Öğrenme Yetkinliği | Makes field studies related to Field Crops, designs, establishes, implements and operates field planting and planting plan. Evaluates and interprets research results. | |
| PLO04 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Finds the source of environmental, biological, technical, production system and economic problems that may adversely affect the achievement of the desired level of yield and quality in field crops agriculture, can develop solutions based on plants and research, and uses information technologies effectively. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Gains experience in the field of Field Crops and Agricultural Engineering, seed production and problems of Field Crops, the development of quality seeds and product storage, and learns about the standards used in the profession. Can make discipline-specific teamwork for solving problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Gains experience in the identification and protection of genetic resources and reproduction methods in field crops. Ability to work effectively in disciplinary and multi-disciplinary teams; acquire the ability to work individually. | 5 |
| PLO07 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Have the ability to acquire new information in the field of Field Crops, research, evaluate, write reports, do consultancy and expertise, prepare and apply design and production reports, make effective presentations, and take and give clear and understandable instructions. | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Field Crops irrigation, fertilization, detection of diseases and pests, mechanization, post-harvest storage etc. has the ability to learn lifelong, access information, follow developments in science and technology, and constantly renew itself on discipline-specific issues. | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | She/He acts in accordance with ethical principles in the fields of Field Crops and Agricultural Engineering, has professional and ethical responsibility and awareness in engineering applications. ( | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Knowledge of applications in business life such as project management, risk management and change management in applications related to Agricultural Engineering; awareness of entrepreneurship, innovation; has knowledge of sustainable development. | |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Information about the effects of Agricultural Engineering practices on health, environment and safety in universal and social dimensions and the problems of the age reflected in the field of engineering; is aware of the legal consequences of engineering solutions. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to tissue culture laboratory and its organization. | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Components of the nutrient medium for tissue culture, chemical used, properties of the chemicals used in tissues culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | Solution preparation techniques in tissue culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Solution preparation techniques in tissue culture Preparation of stock solutions for mineral elements | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 5 | Preparation of stock solutions (vitamins, hormone etc) | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | Techniques of sterilization in tissues culture and homework will be given on this topic | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 7 | Techniques of medium preparation | Reading lecture topic from the course book and the articles about the topicReading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 8 | Mid-Term Exam | Preparation to mid-term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Sterilization of plant materials in tissues culture, isolation of explant and its culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Plant factors affecting succes of tissue culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 11 | Definition, application and uses of embryo culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 12 | Meristem culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 13 | Callus ,cell and protoplast culture | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Techniques of obtaining haploid plants in vitro | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 15 | Somaclonal variation and somatic hybridization | Reading lecture topic from the course book and the articles about the topic | Öğretim Yöntemleri: Anlatım, Tartışma |
| 16 | Term Exams | Preparation to final examination | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation to final examination | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 78 | ||
| Total Workload / 25 (h) | 3,12 | ||
| ECTS | 3 ECTS | ||