TBP316 Tissue Culture Techniques

3 ECTS - 2-0 Duration (T+A)- 6. Semester- 2 National Credit

Information

Code TBP316
Name Tissue Culture Techniques
Term 2024-2025 Academic Year
Semester 6. Semester
Duration (T+A) 2-0 (T-A) (17 Week)
ECTS 3 ECTS
National Credit 2 National Credit
Teaching Language Türkçe
Level Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. RÜŞTÜ HATİPOĞLU
Course Instructor
1 2
Prof. Dr. HAKAN ÖZKAN (A Group) (Ins. in Charge)


Course Goal / Objective

The students learn the basic principles of tissue culture and the tissue culture techniques used in the plants

Course Content

Definition of tissue culture, organization of tissue culture laboratory, preparation of medium and stelization techniques, embryogenesis. organogenesis, tissue cultue techniques used in the plants

Course Precondition

Non

Resources

Hatipoğlu, R. 2010. Lecture notes of Plant tissue culture techniques

Notes

Özcan, S., Gürel, E. ve Babaoğlu, M. 2001. Plant Biotechnology. Vol.I. Selcuk University Foundation Publications


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Learns tools and equipment required in the tissue culture laboratory
LO02 Learns the principles of sterile working
LO03 Learns medium preparation for tissue culture
LO04 Learns isolation and culture of plant explant
LO05 Learns processes of tissue culture techniques
LO06 Identify various sustainability practices.
LO07 To learn how to determine economic threshold level of weeds.
LO08 Economic threshold level of important weeds
LO09 To learn biological control of weeds
LO10 To learn physical and mechanical control of weeds
LO11 To learn chemical control of weeds
LO12 To know herbicides and their mode of action
LO13 To learn herbicides and resistance
LO14 To learn rules of herbicides use and protection of environment.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Have sufficient theoretical knowledge in the fields of Mathematics, Basic Science and Basic Engineering and Agricultural Engineering.
PLO02 Beceriler - Bilişsel, Uygulamalı Recognizes the basic field of Field Crops and also cereals, grain legumes, industrial plants, meadow pasture forage crops and energy crops, has information on the problems and solutions in the physiology, cultivation, biotechnological studies, organic farming, breeding and new variety development of these plants.
PLO03 Yetkinlikler - Öğrenme Yetkinliği Makes field studies related to Field Crops, designs, establishes, implements and operates field planting and planting plan. Evaluates and interprets research results.
PLO04 Yetkinlikler - İletişim ve Sosyal Yetkinlik Finds the source of environmental, biological, technical, production system and economic problems that may adversely affect the achievement of the desired level of yield and quality in field crops agriculture, can develop solutions based on plants and research, and uses information technologies effectively.
PLO05 Beceriler - Bilişsel, Uygulamalı Gains experience in the field of Field Crops and Agricultural Engineering, seed production and problems of Field Crops, the development of quality seeds and product storage, and learns about the standards used in the profession. Can make discipline-specific teamwork for solving problems.
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Gains experience in the identification and protection of genetic resources and reproduction methods in field crops. Ability to work effectively in disciplinary and multi-disciplinary teams; acquire the ability to work individually. 5
PLO07 Yetkinlikler - İletişim ve Sosyal Yetkinlik Have the ability to acquire new information in the field of Field Crops, research, evaluate, write reports, do consultancy and expertise, prepare and apply design and production reports, make effective presentations, and take and give clear and understandable instructions.
PLO08 Yetkinlikler - Öğrenme Yetkinliği Field Crops irrigation, fertilization, detection of diseases and pests, mechanization, post-harvest storage etc. has the ability to learn lifelong, access information, follow developments in science and technology, and constantly renew itself on discipline-specific issues.
PLO09 Yetkinlikler - Alana Özgü Yetkinlik She/He acts in accordance with ethical principles in the fields of Field Crops and Agricultural Engineering, has professional and ethical responsibility and awareness in engineering applications. (
PLO10 Yetkinlikler - Alana Özgü Yetkinlik Knowledge of applications in business life such as project management, risk management and change management in applications related to Agricultural Engineering; awareness of entrepreneurship, innovation; has knowledge of sustainable development.
PLO11 Yetkinlikler - Alana Özgü Yetkinlik Information about the effects of Agricultural Engineering practices on health, environment and safety in universal and social dimensions and the problems of the age reflected in the field of engineering; is aware of the legal consequences of engineering solutions.


Week Plan

Week Topic Preparation Methods
1 Introduction to tissue culture laboratory and its organization. Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
2 Components of the nutrient medium for tissue culture, chemical used, properties of the chemicals used in tissues culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
3 Solution preparation techniques in tissue culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
4 Solution preparation techniques in tissue culture Preparation of stock solutions for mineral elements Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
5 Preparation of stock solutions (vitamins, hormone etc) Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
6 Techniques of sterilization in tissues culture and homework will be given on this topic Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
7 Techniques of medium preparation Reading lecture topic from the course book and the articles about the topicReading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
8 Mid-Term Exam Preparation to mid-term exam Ölçme Yöntemleri:
Yazılı Sınav
9 Sterilization of plant materials in tissues culture, isolation of explant and its culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
10 Plant factors affecting succes of tissue culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
11 Definition, application and uses of embryo culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
12 Meristem culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
13 Callus ,cell and protoplast culture Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
14 Techniques of obtaining haploid plants in vitro Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
15 Somaclonal variation and somatic hybridization Reading lecture topic from the course book and the articles about the topic Öğretim Yöntemleri:
Anlatım, Tartışma
16 Term Exams Preparation to final examination Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Preparation to final examination Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 2 28
Out of Class Study (Preliminary Work, Practice) 14 2 28
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 6 6
Final Exam 1 16 16
Total Workload (Hour) 78
Total Workload / 25 (h) 3,12
ECTS 3 ECTS

Update Time: 03.05.2024 05:43