BB614 Methods for Identifying Genes and Gene Expression in Plants

6 ECTS - 4-0 Duration (T+A)- . Semester- 4 National Credit

Information

Code BB614
Name Methods for Identifying Genes and Gene Expression in Plants
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 4-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 4 National Credit
Teaching Language Türkçe
Level Doktora Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. YILDIZ KAÇAR


Course Goal / Objective

The aim of this course is to the teach methods for identifying genes and gene expression in plants.

Course Content

The contents of this course include structure of nucleic acids, protein translation and proteins, mechanism of transcription in plants and protein translation, gene-protein (enzyme), gene-RNA conversions, promotors and determination of promotors by PCR and sequencing techniques in plants, gene banks for promoters, RT PCR analysis to determine the presence of mRNA, determination of the amount of gene expression by qRT-PCR analysis and evaluation of results, determination of gene expression by micro-array chips, and the creation of micro-array chips, bioinformatics.

Course Precondition

No

Resources

Notes and presentations prepared within the scope of the course content.

Notes

Molecular Biology Mehmet KARATAŞ Regulation of Gene Expression Editors: Gary H. Perdew, John P. Heuvel, Jeffrey M. Peters


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Knows nucleic acids and their structure, protein synthesis.
LO02 Explain and interpret the transcription and translation stages in order to understand gene expression techniques in plants.
LO03 Explain the gene-protein relationships.
LO04 Knows PCR technology and detecting gene expressions with RT PCR. Can compare different gene expression techniques.
LO05 To be able to use, explain, transfer to others the information assimilated in the field of Plant Molecular Genetics in interdisciplinary studies, and examine the results with a critical perspective.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal To be able to develop, deepen, and to reach the original definitions that will bring innovation to the field with the original thinking and/or research of the current and advanced knowledge in his / her field based on his / her master's qualifications. 2
PLO02 Bilgi - Kuramsal, Olgusal Comprehends interdisciplinary interaction related to horticultural plants; achieves original results using information that requires expertise in analyzing, synthesizing and evaluating new and complex ideas 2
PLO03 Bilgi - Kuramsal, Olgusal Evaluates and uses new information in his / her field with a systematic approach 3
PLO04 Bilgi - Kuramsal, Olgusal Can develop a new idea, method, design and/or application, or apply a known idea, method, design and/or application to a different field; can research, comprehend, design and apply an original topic 3
PLO05 Bilgi - Kuramsal, Olgusal Make critical analysis, synthesis and evaluation of new and complex ideas 1
PLO06 Bilgi - Kuramsal, Olgusal To be able to use research methods in his / her field of study 2
PLO07 Bilgi - Kuramsal, Olgusal To develop a new idea, method, design and/or application, or apply a well-known idea, method, design and/or application to a different field independently of the original work to contribute to the progress in the field 2
PLO08 Bilgi - Kuramsal, Olgusal To be able to publish at least one scientific article in national and international refereed journals, or to produce or interpret an original work, to be able to extend the limits of knowledge in the field. 2
PLO09 Bilgi - Kuramsal, Olgusal To Be able to lead in environments that require solving interdisciplinary problems, use problem solving skills in interdisciplinary studies 1
PLO10 Beceriler - Bilişsel, Uygulamalı Develops new ideas and methods related to his / her field by using high-level mental processes such as creative and critical thinking, problem solving and decision making
PLO11 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği In the national and international platforms, they present original views in the discussion of issues related to the field of local and foreign experts, they can defend these views, examine, develop and change the norms leading to social relations and these relations from a critical point of view; they can communicate effectively showing their competence in the field. 2
PLO12 Yetkinlikler - Öğrenme Yetkinliği Specialized in special topics in his / her field 3
PLO13 Yetkinlikler - Öğrenme Yetkinliği To be an information society in which the society lives, to be able to maintain it, to contribute to the solution of the social, scientific, cultural and ethical problems encountered.


Week Plan

Week Topic Preparation Methods
1 Structure of nucleic acids Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
2 Translation and proteins Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
3 Mechanism of transcription in plants Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
4 Translation mechanism in plant Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
5 Gene-protein (enzyme), gene-RNA conversions Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
6 Promotors in Plant Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
7 determination of promotors by PCR and sequencing techniques in plants Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam Preparation of the exam Ölçme Yöntemleri:
Yazılı Sınav
9 Gene banks for promoters Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
10 Microarray analyzes for generation of microarray chips and determination of gene expression Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap
11 RT PCR analysis to determine the presence of mRNA Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
12 Determination of the amount of gene expression by qRT-PCR analysis and evaluation of results Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
13 DNA sequencing Technology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
14 RNA sequencing Technology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
15 Bioinformatics Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
16 Term Exams Preparation of the exam Öğretim Yöntemleri:
Anlatım, Soru-Cevap
17 Term Exams Preparation of the exam Öğretim Yöntemleri:
Anlatım, Soru-Cevap


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 152
Total Workload / 25 (h) 6,08
ECTS 6 ECTS

Update Time: 06.05.2024 02:53