BY611 DNA Cloning and Preparation of Gene Libraries

6 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit

Information

Code BY611
Name DNA Cloning and Preparation of Gene Libraries
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. HATİCE GÜVENMEZ


Course Goal / Objective

Comprehend the principles of DNA cloning and forming gene libraries.

Course Content

PCR, basic principles of gene cloning and DNA analysis, Enzymes used in molecular cloning, gene libraries

Course Precondition

none

Resources

Gene Cloning and Analyses of DNA

Notes

Current Literatures


Course Learning Outcomes

Order Course Learning Outcomes
LO01 understand the pcr and its importance
LO02 comprehend the basic principles of gene cloning and DNA analysis
LO03 comprehend the function of enzymes used in cloning.
LO04 define and compare cloning vectors


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods.
PLO02 Bilgi - Kuramsal, Olgusal Identifies the interdisciplinary interaction that the field is related to. 3
PLO03 Bilgi - Kuramsal, Olgusal Interprets the knowledge gained in the field by integrating the information from different disciplines and creates new information.
PLO04 Bilgi - Kuramsal, Olgusal Carries out a study that requires expertise related to the field independently.
PLO05 Beceriler - Bilişsel, Uygulamalı Evaluates the knowledge and skills acquired at the level of expertise in the field with a critical approach and directs its learning. 4
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Supports the current developments in the field and their studies with quantitative and qualitative data and transmits them both in the field and outside the field systematically in written, oral and visual form.
PLO07 Yetkinlikler - Öğrenme Yetkinliği It supervises the social, scientific, cultural and ethical values in the collection, interpretation, application and announcement stages of the data related to the field and teaches these values. 4
PLO08 Bilgi - Kuramsal, Olgusal The information they absorbed in the field; use problem solving and / or practical skills in interdisciplinary studies. 4
PLO09 Belirsiz It contributes to the solution of social, scientific, cultural and ethical problems encountered in the field and supports the development of these values.
PLO10 Belirsiz Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making.


Week Plan

Week Topic Preparation Methods
1 Basic principles of gene cloning and DNA analysis. reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Soru-Cevap
2 The importance of gene cloning and DNA analysis reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Tartışma
3 PCR and its importance reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Örnek Olay
4 DNA purification from living cells reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Soru-Cevap
5 Manipulation of purified DNA reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Beyin Fırtınası
6 DNA entry into living cells reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Soru-Cevap
7 Vectors for gene cloning reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam An overview Ölçme Yöntemleri:
Sözlü Sınav
9 Behavior of enzymes such as ligase, polymerase, transferase, transcriptase, endonuclease and exonuclease reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım
10 restriction endonucleases reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım
11 Sequences for recognizing the major class II endonucleases reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Tartışma
12 The usability of RFI and RFII type supercoil DNA molecules as vector reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım
13 amplification of genes reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Örnek Olay
14 Methods for detecting clones reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Soru-Cevap
15 Gene's Libraries reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
16 Term Exams An overview Ölçme Yöntemleri:
Sözlü Sınav
17 Term Exams An overview Ölçme Yöntemleri:
Sözlü Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 5 70
Assesment Related Works
Homeworks, Projects, Others 4 6 24
Mid-term Exams (Written, Oral, etc.) 1 10 10
Final Exam 1 10 10
Total Workload (Hour) 156
Total Workload / 25 (h) 6,24
ECTS 6 ECTS

Update Time: 07.05.2024 02:59