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Information
| Code | KM337 |
| Name | Biochemistry Lab 1 |
| Term | 2024-2025 Academic Year |
| Semester | 5. Semester |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. RAMAZAN BİLGİN |
| Course Instructor |
Prof. Dr. RAMAZAN BİLGİN
(A Group)
(Ins. in Charge)
Prof. Dr. GÜZİDE YÜCEBİLGİÇ (A Group) (Asst. Ins.) |
Course Goal / Objective
The aim of this course is to introduce the application and evaluation of basic biochemical analysis methods and experimental results with theoretical knowledge.
Course Content
In this course, introduction of the rules and devices to be followed in the laboratory, Carbohydrates and general recognition reactions, Determination of glucose in blood, Properties and reactions of amino acids, Determination of amino acids by paper chromatography, Determination of amino acids by titrimetric method (formol titration), Titration curves of amino acids: isoelectric point determination. , General reactions of proteins, Determination of protein in serum, Determination of salivary amylase activity, Determination of serum alkaline phosphatase activity, General reactions of lipids, Determination of cholesterol in serum are covered.
Course Precondition
None
Resources
Biochemistry Lab. Notes, Prof.Dr. Ramazan Bilgin
Notes
Biochemistry Lab. Notes, Prof.Dr. Ramazan Bilgin
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Recognizes biochemical analysis methods |
| LO02 | Understands the evaluation and interpretation of experimental results. |
| LO03 | Understands carbohydrates and general recognition reactions |
| LO04 | Understands general properties and reactions of aminoacids |
| LO05 | Understands general reactions of proteins |
| LO06 | Understands determination of amylase activity in saliva |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Relates his/her knowledge in the field of chemistry to life and industry by relying on his/her knowledge | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Evaluates the developments in the world by following and perceiving the orientations in the industry. | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains awareness of lifelong learning and renews his/her knowledge. | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Access to information and associate their knowledge with other fields of science. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Understands the need to combine information in the application of information and perceives the total quality awareness. | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | S/he has knowledge in the use of resources, in the creation of technology depending on the country's resources. | 3 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | S/he conveys what s/he knows to people close or distant to the subject by concretising. | 3 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Gains the ability to access new information and technology. | 3 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Learns to think creatively and produce solutions to problems in the field. | |
| PLO10 | Yetkinlikler - Öğrenme Yetkinliği | To be able to associate information by having the ability of reasoning and transfer what they know to practice. | 4 |
| PLO11 | Beceriler - Bilişsel, Uygulamalı | Sets up laboratory equipment and makes decisions alone. | 4 |
| PLO12 | Bilgi - Kuramsal, Olgusal | To be able to follow the current technology in the field of chemistry and gain added value. | 3 |
| PLO13 | Bilgi - Kuramsal, Olgusal | Follows the developments, gains the ability to follow the directions in the field. | 3 |
| PLO14 | Bilgi - Kuramsal, Olgusal | Knowing information sharing and participating in teamwork and directing the team. | 2 |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Gains universal thinking and critical perspective. | |
| PLO16 | Bilgi - Kuramsal, Olgusal | Starts to have a say in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction of rules and devices that should be observed in laboratories | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Carbohydrates and general recognition reactions | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Glucose determination in blood | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | General properties and reactions of aminoacids | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Determination of aminoacids by paper chromatography | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Determination of aminoacids by titration (formol titration) | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Titration curves of amino acids:Isoelectric point determination | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | General reactions of proteins | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 10 | Protein determination in blood serum | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | Determination of amylase activity in saliva | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 12 | Determination of alkaline phosphatase in blood serum | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 13 | General reactions of lipids | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 14 | Cholesterol determination in blood serum | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 15 | Make-up experiments | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 16 | Term Exams | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 58 | ||
| Total Workload / 25 (h) | 2,32 | ||
| ECTS | 2 ECTS | ||