TÜRKÇE
ENGLISH
Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (MASTER) (WITH THESIS) (INTERDISCIPLINARY) | |
| Code | BT517 |
| Name | Plant Tissue Culture |
| Term | 2024-2025 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof.Dr. SAADET BÜYÜKALACA |
| Course Instructor |
Prof. Dr. HATIRA TAŞKIN
(Güz)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
To give general and practical information about plant tissue cultures.
Course Content
Tissue culture-related definitions, techniques used in plant tissue culture, preparation of plant nutrient media, isolation and transferring of plant tissues, culture conditions, acclimatization of in vitro plants to environment, meristem culture, shoot tip culture, cell and callus culture.
Course Precondition
None
Resources
presentations prepared and publications of recent years
Notes
Bitki Biyoteknolojisi (Rüştü Hatipoğlu)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Obtains the conceptual and theoretical knowledge about plant tissue culture. |
| LO02 | Knows the factors affecting the sucsess of plant tissue culture, improvement techniques and methods to increase the success of plant tissue cultures. |
| LO03 | Knows theoretical and practical methods for different researches. |
| LO04 | Knows to transform theoretical knowledge in laboratory studies, experiments. |
| LO05 | Improves new methods to solve present problems. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on undergraduate level qualifications, they develop their knowledge at the level of expertise in the same or a different field. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 5 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 3 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO06 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 2 |
| PLO07 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 4 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | History of plant tissue cultures | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Tissue culture laboratory techniques (preparation of nutrient medium, sterilization techniques of media-explant and equipment used in tissue culture, infection sources in tissue e culture) | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | Meristem culture | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Production of disease-free plant | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | Embryo culture | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | Anther, ovule and microspore culture | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 7 | Callus and cell suspension cultures | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Preparation to mid-term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Direct and indirect organogenesis | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Direct and indirect embryogenesis | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım |
| 11 | Bioreactors and artificial seed production | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım |
| 12 | Germplasm preservation | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Somaclonal variations | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Acclimatization of in vitro plants | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | General discussion about tissue culture techniques and use of these techniques in Horticulture | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Preparation to final exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation to final exam | Ölçme Yöntemleri: Yazılı Sınav |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 40 |
| General Assessment | ||
| Midterm / Year Total | 100 | 40 |
| 1. Final Exam | - | 60 |
| Grand Total | - | 100 |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||