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Information
| Code | BT0097 |
| Name | Basic Molecular Biology Technigues |
| Term | 2024-2025 Academic Year |
| Term | Fall |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, analysis and interpretation of PCR and QF PCR results, PCR types, Real Time PCR methods and applications, Blotting techniques, learning application areas (PCR RFLP, Sequence analysis, Gene cloning)
Course Content
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, analysis and interpretation of PCR and QF PCR results, PCR types and Application fields (Mutation screening, PCR RFLP, Sequence analysis, Gene cloning), Real Time PCR methods and applications, Blotting techniques
Course Precondition
None
Resources
Medical Biology and Genetics, Editor: Prof. Dr. Halil Kasap, Akademisyen Bookstore, 4th edition, 2020
Notes
Pazarbaşı A, Kasap M, Vardar MA, Alptekin D, Güzel AI, Lüleyap HÜ, Altıntaş A, Doran F.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the structure of genetic material |
| LO02 | Knows genetic diseases and genes that cause diseases |
| LO03 | Can perform molecular genetic examinations and analyzes |
| LO04 | It can isolate DNA from any tissue |
| LO05 | To know the basic of PCR |
| LO06 | Perform and interpret agarose and polyacrylamide gel electrophoresis |
| LO07 | Can measure DNA obtained from tissue in spectrophotometer |
| LO08 | Can interpret a scientific article and learn the writing of the article |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 5 |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 5 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 5 |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 5 |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Learning genome analysis methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Mechanism of polymerase chain reaction (PCR) | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Optimization of the polymerase chain reaction (PCR) 2 | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | DNA isolation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Agarose and polyacrylamide gel electrophoresis of PCR products | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | how to prepare solutions and gels and staining gels | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Understanding and applying the QF PCR method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid term exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 9 | PCR types | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Analysis and interpretation of PCR and QF PCR results | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Learning Real Time PCR methods and applications | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Blotting techniques | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Application areas (Mutation screening, PCR RFLP) | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Sequence analysis | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Gene cloning | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Final exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 17 | Final exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||