BT052 Gene Cloning in Microorganisms

6 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit

Information

Code BT052
Name Gene Cloning in Microorganisms
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. BAHRİ DEVRİM ÖZCAN


Course Goal / Objective

The aim of this course is to make students comprehend gene cloning techniques in microorganisms and to understand the biotechnological, agricultural and industrial importance of gene cloning in microorganisms

Course Content

Genome structure of microorganisms, cloning vectors, cloning enzymes, cloning of PCR products, gene transfer methods, yeast cloning vectors, biotechnological, agricultural and industrial importance of gene cloning in microorganisms

Course Precondition

No prerequisite

Resources

Gene Cloning and DNA Analysis, Fifth Edition, 2006, T.A. Brown. Blackwell Publishing, UK. Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999

Notes

Moleküler Biyoteknoloji, Rekombinant DNA'nın Prensipleri ve Uygulamaları, B.R. Glick, C.L. Patten, Beşinci Baskıdan Çeviri, 2021. Çeviri Editörü: T. Kankılıç, Palme Yayınları, Ankara


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Knows the theory of gene cloning techniques in microorganisms
LO02 Isolates a gene from the genomes of microorganisms
LO03 Recognize and use cloning vectors
LO04 Recognize and use DNA modifying enzymes
LO05 Clones a microbial gene
LO06 Knows biotechnological, agricultural and industrial importance of gene cloning in microorganisms


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. 1
PLO02 Beceriler - Bilişsel, Uygulamalı To identifiy the interdisciplinary interaction of the field and to solve use of the methods.
PLO03 Beceriler - Bilişsel, Uygulamalı Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information
PLO04 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. 2
PLO05 Yetkinlikler - Öğrenme Yetkinliği To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field.
PLO06 Yetkinlikler - İletişim ve Sosyal Yetkinlik To solve the problems encountered in the field and cominicate by using research methods. 3
PLO07 Yetkinlikler - Alana Özgü Yetkinlik To gain the ability to develop and deepen the knowledge in the field of biotechnology 3
PLO08 Yetkinlikler - Alana Özgü Yetkinlik Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field.
PLO09 Yetkinlikler - Öğrenme Yetkinliği Knows the ethical rules to be considered while obtaining biotechnological products.
PLO10 Yetkinlikler - İletişim ve Sosyal Yetkinlik He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field.


Week Plan

Week Topic Preparation Methods
1 Genome structure of microorganisms Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
2 Microbial coning vectors Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
3 Chromosomal and plasmid DNA isolation from microorganisms Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
4 DNA-modifying enzymes and their use in cloning 1 Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
5 DNA-modifying enzymes and their use in cloning 2 Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
6 Competent cell preparation from microorganisms Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
7 Preparation for mid-term exam Preparation for mid-term exam Öğretim Yöntemleri:
Anlatım, Soru-Cevap
8 Mid-Term Exam Mid-term exams Ölçme Yöntemleri:
Yazılı Sınav
9 Shotgun-based cloning Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
10 PCR-based cloning Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
11 Gene transfer methods in microorganisms (transformation, electroporation, protoplast fusion) Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
12 Selection and analysis of recombinant colonies Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
13 Biotechnological importance of gene cloning in microorganisms Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
14 Agricultural importance of gene cloning in microorganisms Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
15 Industrial importance of gene cloning in microorganisms Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 Öğretim Yöntemleri:
Anlatım
16 Term Exams Term exams Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Term exams Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 5 70
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 15 15
Final Exam 1 30 30
Total Workload (Hour) 157
Total Workload / 25 (h) 6,28
ECTS 6 ECTS

Update Time: 14.05.2024 04:49