BT0099 Animal Cell Culture Applications-I

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit

Information

Code BT0099
Name Animal Cell Culture Applications-I
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Doktora Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator


Course Goal / Objective

Learning the Application Areas of Basic Methods in Cell Culture

Course Content

Cell culture Techniques, Devices Used in Cell Culture and Their Properties, Cell Culture Sowing, Propagation, Passaging and Freezing Methods, Cell Culture Safety Conditions

Course Precondition

none

Resources

Freshney RI., Culture of Animas Cells:A Manual of Basic Technique

Notes

Freshney RI., Culture of Animas Cells:A Manual of Basic Technique


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Can choose a medium to support cell culture
LO02 Has knowledge of the basic features of cell culture
LO03 Knows the differences of cell culture types and has information about comparing cell structures
LO04 Has the knowledge to choose the appropriate medium for the structure of the cells according to their types
LO05 Define the coordination of medium and support medium in cell culture.
LO06 Can identify contaminations that may occur in cell culture
LO07 Determine what contamination may be in cell culture and evaluate the appropriate solution.
LO08 Has knowledge of the safety of the person working in cell culture applications
LO09 Define the methods that can be used in living cell determination
LO10 Has knowledge about the methods of using cells obtained from cell culture in molecular studies.
LO11 Has knowledge about the cryopreservation and storage conditions of sub-passages in cell culture


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. 5
PLO02 Bilgi - Kuramsal, Olgusal Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas.
PLO03 Beceriler - Bilişsel, Uygulamalı Evaluates, uses and transfers new information in the field with a systematic approach. 5
PLO04 Beceriler - Bilişsel, Uygulamalı Makes critical analysis, synthesis and evaluation of new and complex thoughts.
PLO05 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Makes leadership in environments that require the analysis of original and interdisciplinary problems.
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work.
PLO07 Yetkinlikler - Öğrenme Yetkinliği Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making.
PLO08 Yetkinlikler - İletişim ve Sosyal Yetkinlik He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field.
PLO09 Yetkinlikler - Alana Özgü Yetkinlik Conducts scientific research in national and international scientific research groups. 4
PLO10 Yetkinlikler - Alana Özgü Yetkinlik Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. 4


Week Plan

Week Topic Preparation Methods
1 What is cell culture? What are the cell culture uses? Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
2 Cell culture types Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
3 Techniques used in cell culture Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
4 Cell culture media Literature browsing and reading Öğretim Yöntemleri:
Soru-Cevap, Anlatım
5 Support media in cell culture Literature browsing and reading Öğretim Yöntemleri:
Soru-Cevap, Anlatım
6 Cell culture contaminations Literature browsing and reading Öğretim Yöntemleri:
Soru-Cevap, Anlatım
7 Measures to be taken against cell culture contamination Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
8 midterm Literature browsing and reading Ölçme Yöntemleri:
Performans Değerlendirmesi
9 Safety conditions in cell culture Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
10 Cell viability determination methods Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
11 Molecular methods that can be used after Cell Culture Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
12 Cell culture freezing methods Literature browsing andreading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
13 Storage conditions of frozen cells Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
14 Cell count Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
15 Flask selection according to application method in cell culture Literature browsing and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap
16 Final exam Literature browsing and reading Ölçme Yöntemleri:
Performans Değerlendirmesi
17 Final exam Literature browsing and reading Ölçme Yöntemleri:
Performans Değerlendirmesi


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 2 2
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS

Update Time: 14.05.2024 05:03