ST011 Molecular Biological Methods in Aquaculture

6 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit

Information

Code ST011
Name Molecular Biological Methods in Aquaculture
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. MAKBULE BAYLAN


Course Goal / Objective

be teached and introduced of molecular biological methods used in aquaculture

Course Content

DNA isolation and analysis (DNA exraction of bacteria yeast and fish), Bacterial transformation (Chemical transformation, transformation by electroporation (electrotransformation)), DNA amplification by Polymerase Chain Reaction (PCR), isolation of proteins, purification and enzymatic analysis of proteins.

Course Precondition

None

Resources

Temizkan G., Arda N., 2004. Methods Used in Molecular Biology, Nobel Kitapevi, 613p

Notes

Temizkan G., Arda N., 2021. Basic and Advanced Molecular Biology Methods-Genomic and Proteomic Analysis.Nobel Kitapevi


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Ensuring do studying entirely on one s own of people wanting to join a study in genetics and biotechnology in aquaculture
LO02 Have detailed information about DNA isolation
LO03 Have detailed information about PCR amplification of DNA
LO04 Gain knowledge of the laboratory
LO05 Have knowledge about chemicals
LO06 Have detailed information about the methods to be used.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Improves theoretical and practical knowledge in the field of Marine and Inland Water Biology and Fisheries Basic Sciences.
PLO02 Bilgi - Kuramsal, Olgusal Comprehends interactions between Fisheries Basic Sciences and other disciplines. 3
PLO03 Bilgi - Kuramsal, Olgusal Determines strategies and investigates methods about their field of study in Fisheries Basic Science.
PLO04 Bilgi - Kuramsal, Olgusal Produces new information and theories by interpreting and synthesising the information from other disciplines and uses the theoretical and practical information from their field of study in Fisheries Basic Science. 2
PLO05 Bilgi - Kuramsal, Olgusal Collects data, interprets results and suggests solutions by using dialectic research methodology in the certain field of Marine and Inland Water Biology and Fisheries Basic Sciences. 3
PLO06 Bilgi - Kuramsal, Olgusal Independently plans, designs and performs a certain project in the field of Fisheries Basic Sciences. 4
PLO07 Bilgi - Kuramsal, Olgusal Produces solutions by improving new strategic approaches and taking responsibilities for the potential problems in the field of study as an individual or team member. 4
PLO08 Beceriler - Bilişsel, Uygulamalı Determines the requirements for Fishery Basic Science education, reaches the resources, critically interpretes knowledge and skills and gains experience to direct the education. 2
PLO09 Beceriler - Bilişsel, Uygulamalı Has positive stance on the lifelong education and uses it for the public benefit by using the gained theoretical and practical knowledge in the field of Marine and Inland Water Biology and Fisheries Basic Sciences. 1
PLO10 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Follows the current topics and improvements in the field of Fisheries Basic Sciences, publishes and presents the research results, contributes to constitution of a public conscience in the field of interest.
PLO11 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Effectively communicates about the field of Marine and Inland Water Biology and Fisheries Basic Sciences by using written and oral presentation tools, follows up and criticizes the meetings and seminars.
PLO12 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Follows up international publications and communicates with international collaborators by using language skills.
PLO13 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Uses the communication and information technologies about the field of interest in an advanced level. 4
PLO14 Yetkinlikler - Öğrenme Yetkinliği Conforms, controls and teaches social, cultural and scientific ethics in the investigation and publication process of the data related with the field of interest. 2
PLO15 Yetkinlikler - Öğrenme Yetkinliği Improves strategies, politics and application codes by following scientific and technological developments on the certain field of Marine and Inland Water Biology and Fisheries Basic Sciences. Investigates and extends the results on behalf of public in frame of total quality management process. 3
PLO16 Yetkinlikler - Öğrenme Yetkinliği Uses the abilities and experiences on applications and solving problems that gained during the MSc education for the interdisciplinary studies. 3


Week Plan

Week Topic Preparation Methods
1 DNA isolation and analysis, Chrosome DNA isolation Reading at lecture note Öğretim Yöntemleri:
Anlatım, Alıştırma ve Uygulama
2 Chromosomal DNA isolation from bacteria Reading at lecture note Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
3 Chromosomal DNA isolation from yeast Reading at lecture note Öğretim Yöntemleri:
Anlatım
4 Chromosomal DNA isolation from fish Reading at lecture note Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
5 transformation by chemical aplication in bacteria Reading at lecture note Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
6 transformation by electroporation in bacteria Reading at lecture note Öğretim Yöntemleri:
Anlatım
7 DNA amplification by Polymerase Chain Reaction (PCR) Reading at lecture note Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam preparation for term exam Ölçme Yöntemleri:
Yazılı Sınav
9 formation mechanism of Polymerase Chain Reaction, basic components of PCR, functioning of PCR Reading at lecture note Öğretim Yöntemleri:
Anlatım
10 Amplification of correct pruduct with PCR, identification and analysis of PCR products Reading at lecture note Öğretim Yöntemleri:
Anlatım
11 prevention from contamination in PCR, types of PCR Reading at lecture note Öğretim Yöntemleri:
Anlatım
12 Protein isolation, purification and enzymatic analysis Reading at lecture note Öğretim Yöntemleri:
Anlatım
13 Tools, materials and chemicals Reading at lecture note Öğretim Yöntemleri:
Anlatım
14 Preparation of protein extract Reading at lecture note Öğretim Yöntemleri:
Anlatım
15 Protein extraction, storage conditions, extraction of fish tissue Reading at lecture note Öğretim Yöntemleri:
Anlatım
16 Term Exams preparation for final exam Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams preparation for final exam Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 5 70
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 15 15
Final Exam 1 30 30
Total Workload (Hour) 157
Total Workload / 25 (h) 6,28
ECTS 6 ECTS

Update Time: 16.05.2024 03:38