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Information
| Code | HİS518 |
| Name | Histological Techniques 2 Laboratory |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-4 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 6 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. YURDUN KUYUCU |
Course Goal / Objective
Applying the immunohistochemical techniques and observing the basic techniques of molecular biology.
Course Content
Applying basic principles of immunohistochemical techniques, antibody choise, tissue prosessing, staining methods, evaluation and observing basic principles of molecular biology.
Course Precondition
Histological Techniques 2
Resources
Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Notes
Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Applies fixation and immunohistochemical staining methods |
| LO02 | Observes application of basic methods for molecular biology. |
| LO03 | Evaluates molecular biological results |
| LO04 | Applies the basic steps of tissue processes. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Tell the basic histological features of cells and tissues. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Can establish connection betwwen the structure and function of the cells. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Have knowledge about the histological properties of organ systems. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Have knowledge about embryo development, development disorders and mechanisms. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Define light and electron microscopic tissue preparation and examination methods. | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has knowledge about general principles of cell culture, histochemistry and immunohistochemistry. | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Prepare tissues for light and electron microscopic analysis. | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Apply basic histochemical staining methods. | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Can distinguish tissue and organ systems at light microscopic level. | |
| PLO10 | Bilgi - Kuramsal, Olgusal | He / she carries out his / her study as an individual or a team member. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Do the tasks given within the scope of research projects. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Develop a positive attitude and behavior related to lifelong learning. | |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Identifies learning needs and resources, and guides them by using resources effectively. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Evaluate the knowledge and skills at the graduate level with a critical approach. | |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Use information and communication technologies at the level required by the field. | |
| PLO16 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Communicate effectively. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transfer their ideas and solutions by quantitative and qualitative datas by supporting the current developments and their studies orally and visiually | |
| PLO18 | Yetkinlikler - Öğrenme Yetkinliği | Knows the importance of ethical principles in conducting research studies, evaluating and sharing the results. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Praparing solutions of method. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 2 | Obtaining the tissues. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 3 | Applying the basic steps of tissue processes. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 4 | Taking sections. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 5 | Applying immunohistochemical method. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 6 | Examining slides in microscope. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 7 | Evaluation of immunohistochemical results. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Obtaining material for molecular biological techniques. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Observing gel electrophoresis technique. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri |
| 11 | Observing polymerase chain reaction procedures | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri |
| 12 | Observing Western Blot technique | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri |
| 13 | Observing Hybridisation technique. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri |
| 14 | Observing ELISA method. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri |
| 15 | Evaluation of results. | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama, Gösteri |
| 16 | Term Exams | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 10 | 10 |
| Final Exam | 1 | 14 | 14 |
| Total Workload (Hour) | 150 | ||
| Total Workload / 25 (h) | 6,00 | ||
| ECTS | 6 ECTS | ||