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Information
| Code | VTS236 |
| Name | Molecular Techniques used in Pathology |
| Term | 2024-2025 Academic Year |
| Semester | 4. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | FE Field Education Courses E Elective |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. Mehmet Önder KARAYİĞİT |
| Course Instructor |
Prof. Dr. MEHMET HALIGÜR
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
It is aimed to gain knowledge and skill about the molecular pathology technics using in the pathology department.
Course Content
Molecular pathology terminology and techniques, DNA, RNA and protein extraction and purification in tissue, PCR and application areas in pathology, Mutations and mutagens, DNA typing of the anatomic pathology, Tumoriogenesis
Course Precondition
NONE
Resources
Moleküler Biyoloji Teknileri, Doç. Dr. Ajda Çoker Gürkan Doç. Dr. Pınar Obakan Yerlikaya Doç. Dr. Elif Damla Arısan, 2017; Moleküler Biyoloji Teknileri, Doç. Dr. Ajda Çoker Gürkan Doç. Dr. Pınar Obakan Yerlikaya Doç. Dr. Elif Damla Arısan, 2017; Lecture notes
Notes
Lecture notes and current literature
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explain the importance of the relationship between routine pathology and molecular pathology |
| LO02 | Recognize molecular techniques used in the pathology laboratory. |
| LO03 | Describes the basic structures of molecular biology |
| LO04 | Infers genes involved in carcinogenesis and their treatments |
| LO05 | The use of PCR in the pathology laboratory expresses the usage technique and stages. |
| LO06 | Identify the materials used in the pathology laboratory. |
| LO07 | List the steps of DNA, RNA and protein extraction |
| LO08 | Makes inferences about mutation |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Distinguishes animal species and breeds based on their structural (morphological), functional (physiological, biochemical) and behavioral characteristics and whether they can be consumed for food purposes. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Explains the sources, physical and chemical properties and preparation of drugs. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Describes the effects and side effects of medications on patients | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Explains the formation mechanisms of animal diseases and their pathological effects on the body. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | It makes efficiency evaluations among animal breeds and, when necessary, carries out selection, hybridization and artificial insemination at a level that can carry out breeding work. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Disposes of dead animal material, tissue samples, medical and chemical waste appropriately in accordance with international, national laws and regulations. | 4 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | It determines the correct approach area for the patient by evaluating the examination, operation etc. practices in the clinic according to the topographic anatomy. | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Uses biochemical parameters in diagnostic processes by evaluating the information obtained with cause-effect relationships. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Writes prescriptions in accordance with diagnosis and treatment protocols for diseases and poisoning in animals. | 3 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Implements necessary measures, including biosecurity, to control infectious diseases. | |
| PLO11 | Beceriler - Bilişsel, Uygulamalı | Performs necropsy to evaluate findings in dead animals, directs tissues to the necessary laboratory according to appropriate storage methods and prepares a report. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Monitors patients in the post-operative process according to the main treatment methods of surgical diseases in animal species. | |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Applies sedation, anesthesia and pain management methods safely according to animal species. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Correctly applies biosafety principles such as sterilization of clinical and laboratory equipment and clothing. | |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | It regulates the care and feeding protocols according to animal species and supervises the consumption of animal foods produced for human consumption or foods containing animal products in their composition. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Evaluates laboratory results of animal diseases and applies correct diagnosis, prognosis and treatment methods based on the data obtained. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | He/she is conscious of performing his/her profession by being aware of his/her powers and responsibilities. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Examines scientific techniques and methods according to facts related to veterinary services and takes analysis or methodological responsibility for problems. | 5 |
| PLO19 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It uses adequate technology in the hygienic production, manufacturing, processing, consumption and sales of food by conducting health control of animal food products produced for human consumption. | |
| PLO20 | Yetkinlikler - Öğrenme Yetkinliği | Lists the methods of producing and using scientific knowledge in his/her professional field. | |
| PLO21 | Yetkinlikler - Öğrenme Yetkinliği | Uses updated legislation regarding veterinary services when necessary. | |
| PLO22 | Yetkinlikler - Öğrenme Yetkinliği | Performs an examination appropriate to the case, applying the contribution of imaging methods to diagnosis and radiation safety in accordance with current regulations. | |
| PLO23 | Yetkinlikler - Öğrenme Yetkinliği | Keeps clinical and patient records using proper and scientific methods. | |
| PLO24 | Yetkinlikler - Öğrenme Yetkinliği | By observing the basic principles of medicine, it develops management methods and appropriate management plans for animal production enterprises. | |
| PLO25 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Implement social projects and plans with social awareness, based on professional skills and authority with a computer usage license, advanced computer software. | |
| PLO26 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Works in cooperation with other colleagues by following the world agenda within the framework of professional ethics and deontology rules. | |
| PLO27 | Yetkinlikler - Alana Özgü Yetkinlik | Produces solutions to field-specific problems in line with scientific data/evidence. | |
| PLO28 | Yetkinlikler - Alana Özgü Yetkinlik | He/she has the ethical values of the veterinary profession and defends these values. | |
| PLO29 | Yetkinlikler - Alana Özgü Yetkinlik | Takes appropriate precautions by clinically recognizing zoonosis and notifiable diseases. | |
| PLO30 | Yetkinlikler - Alana Özgü Yetkinlik | Determines the methods of diagnosis, prevention, treatment and operation of diseases in different animal species. | |
| PLO31 | Yetkinlikler - Alana Özgü Yetkinlik | In emergency cases, intervenes in all animals and applies first aid methods. | |
| PLO32 | Yetkinlikler - Alana Özgü Yetkinlik | Makes judgments on animal husbandry principles and breeding by assessing the physical condition, welfare and rearing conditions of domestic animals, communicating appropriately with animal owners and colleagues. | |
| PLO33 | Yetkinlikler - Alana Özgü Yetkinlik | Independently interprets articles and presentations published in other scientific fields related to Veterinary Medicine to maintain professional development and skills. | |
| PLO34 | Yetkinlikler - Alana Özgü Yetkinlik | When necessary, euthanizes animals using appropriate methods and respecting the feelings of animal owners. | |
| PLO35 | Yetkinlikler - Alana Özgü Yetkinlik | By mastering the concepts of occupational safety in terms of the workplace and the personnel working with it, they have the competence to apply and defend it. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to pathology, rules to be observed in the laboratory and materials used | Lecture notes, book | Öğretim Yöntemleri: Anlatım |
| 2 | The structure and functions of nucleic acids | Lecture notes, book | Öğretim Yöntemleri: Anlatım |
| 3 | Cell cyclus, DNA replication, protein synthesis | lecture notes, internet, visual data | Öğretim Yöntemleri: Anlatım, Gösteri |
| 4 | Mitosis and meiosis | Lecture notes, visual data, internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 5 | Stages of mitosis and meiosis | Lecture notes, video demonstration | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 6 | Extraction of DNA, RNA and protein and isolation | Lecture notes, visual data, book | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 7 | PCR and its areas of use in pathology, PCR formation mechanism, basic components of PCR | Lecture notes, book | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Lecture notes, book, internet | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 9 | Mutation and mutagens, types of mutation, DNA repair, DNA typing in anatomic pathology | Current articles, lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Cytogenetic, cytogenetic in pathology, molecular genetic pathology | Current articles, lecture notes, book | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Immunohistochemical methods and use in pathology, immunohistochemical markers | Book, lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Tumoriogenesis, p53 gene in tumorigenesis, genetic treatment methods | book, current articles, lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Tumors related genes, protooncogenes, tumor suppressor genes, DNA repair genes, apoptosis genes | lecture notes, book | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Cancer genetics, molecular biology of cancer | lecture notes, book | Öğretim Yöntemleri: Anlatım |
| 15 | Microarray technology, Electrophoretic methods and blotting, display methods of the cell cycle phases | lecture notes, book, visual data | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 16 | Term Exams | Lecture notes | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 17 | Term Exams | Lecture notes | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 4 | 4 |
| Total Workload (Hour) | 76 | ||
| Total Workload / 25 (h) | 3,04 | ||
| ECTS | 3 ECTS | ||