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Information
| Code | TB607 |
| Name | Laboratory Techniques in Biotechnology |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
The aim of this course is to provide theoretical information and practice on working techniques in biotechnology laboratories and biotechnological methods used in plants.
Course Content
536 / 5.000 This course covers the explanation of the concepts of biotechnology, plant biotechnology, in vitro culture, introduction of the equipment of the biotechnology laboratory, nutrient media used in in vitro culture, preparation of macro and micro element stock solutions, preparation of growth regulator and vitamin stock solutions, sterilization principles of glassware and metal materials, preparation of nutrient media, sterilization of plant material, embryo culture, anther culture, DNA isolation, PCR analysis.
Course Precondition
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Resources
Hatipoğlu, R. 1993. Biyoteknolojide Laboratuar teknikleri ders notu
Notes
Hatipoğlu, R. 1993. Biyoteknolojide Laboratuar teknikleri ders notu
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the concepts of biotechnology, plant biotechnology, in vitro culture, genetic engineering, molecular markers |
| LO02 | Have knowledge about the infrastructure and equipment required in a biotechnology laboratory. |
| LO03 | Can apply topics such as preparation of nutrient medium, sterile work, some in vitro culture techniques, DNA isolation, PCR, etc. |
| LO04 | Has knowledge of sterile work and safety precautions |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Graduates become a specialist on the field crops area by improving their skills. | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | They comprehend interdisciplinary interaction in his specialization area. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | They can follow the latest developments in field crops area and get access to the knowledge source, gather this knowledge, reach new knowledge and synthesize by evaluating available studies and follow the innovations in his field. | 2 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | They can synthesize and interpret interdisciplinary knowledge by using theoretical and practical skills at a specialist level in field crops area. | 3 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They find solutions for problems related to field crops by using research methods and establish cause effect relationships. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They incorporate their knowledge on his special area by combining their knowledge with those from the other scientific areas and produce new knowledge and also solve the problems by using scientific research methods. | 5 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They determine a problem independently in field crops subject, provide solutions, evaluate the results and implement whenever required. | |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They are qualified to solve a problem in their field. | |
| PLO09 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They are equipped with analytical and critical thinking ability to direct their learning and to conduct advanced studies in their fields independently. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They transfer current developments and their own studies in the field crops area systematically to the his working group and to different groups from other study fields orally or visually by supporting them with qualitative and quantitive data. | 4 |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | They study and improve the social relationships and standards leading these relationship by a critical point of view and take action when needed. | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | They are able to understand and translate an article written in a foreign language. | |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | They develop policy, strategy and experimental plans related to field crops area and evaluate obtained results within the framework of quality processes. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Explanation of Biotechnology, Plant Biotechnology, and In Vitro Culture Concepts | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 2 | Introducing Biotechnology Laboratory Equipment | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 3 | Nutrient Media for In Vitro Culture | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 4 | Preparing Macro and Microelement Stock Solutions | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 5 | Preparing Growth Regulator and Vitamin Stock Solutions | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 6 | Principles of Sterilizing Glassware and Metalware | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 7 | Nutrient Media Preparation | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Pre-reading | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Principles of Working in a Sterile Cabin | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 10 | Sterilization of Plant Material: A Homework Assignment Will Be Given | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 11 | Embryo culture | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 12 | Anther culture | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 13 | DNA Isolation | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 14 | PCR analysis of DNA with pre-determined quality and quantity | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 15 | Analyzing PCR Results | Pre-reading | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Pre-reading | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Pre-reading | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 24 | 24 |
| Total Workload (Hour) | 144 | ||
| Total Workload / 25 (h) | 5,76 | ||
| ECTS | 6 ECTS | ||