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Information
| Code | VPT 503 |
| Name | Light Microscopic Preparation, Cytology and Staining Techniques |
| Term | 2024-2025 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
*Preparation of the sample for pathology for microscopic examination, * What are the materials required for tissue tracking and their supply * Sectioning and painting methods, *How the cytological preparation is prepared, *Providing skills on how to stain and examine the cytological preparation
Course Content
It includes determination of the material coming to the laboratory for histopathological examination, decalcification, preparation of preparations, cytology and staining methods.
Course Precondition
NONE
Resources
Ö Aytekin, Temel Histoloji, Nobel Akademik Yayıncılık, 2014
Notes
Lecture notes and current literature
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Lists sample acceptance and follow-up |
| LO02 | Defines sample acceptance-observation-recording-photography and preparation for follow-up. |
| LO03 | Explain what the materials required for the follow-up are, how to prepare them (preparation of 70, 80, 90% alcohol), |
| LO04 | It describes tissue tracking, blocking and sectioning. |
| LO05 | Lists the staining methods to be applied to the sections taken.. |
| LO06 | Explains the importance of painting techniques |
| LO07 | Comments on errors in stained tissues |
| LO08 | Explains the importance of learning tissue preparation and staining techniques well. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Defines the basic concepts in the field of veterinary pathology, | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Explain species-specific diseases to the field of veterinary pathology | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Defines and uses basic pathology concepts specific to the field of veterinary pathology, | 3 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Learns laboratory procedures and evaluation of samples related to the field of veterinary pathology. | 5 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Examines specimens related to veterinary pathology under microscope | 3 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Interprets the results of samples in the field of Veterinary Pathology | |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Conducts scientific research to respond to emerging problems in Veterinary, Medicine and health sciences | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Diagnoses the disease in the field of veterinary pathology and ensures that the necessary measures are taken to prevent the disease, | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | It provides the diagnosis of tumors according to animal species in the veterinary field, | |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Uses diagnostic methods within the framework of the latest scientific developments in the field of veterinary pathology, | 3 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Designs a field survey to identify animal diseases and zoonotic diseases, determines and manages the sampling method, | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Determines the diagnosis of animal diseases in the field of veterinary pathology and cooperates and consults with various units in the decision-making process in treatment, | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develop critical thinking skills, evaluate evidence critically and make decisions using ways to reach evidence, | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | It reports data on the prognosis of many types of diseases and tumors detected in the field of veterinary pathology. | |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Provides consultancy using effective communication skills, takes part in teamwork in research, defends scientific ethical rules | |
| PLO16 | Yetkinlikler - Alana Özgü Yetkinlik | Explains the concepts in the field of epidemiology, directs clinical and field research from the beginning, develops methods for determining disease risk factors and disease burdens, provides consultancy in selecting appropriate diagnostic tests, |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Course introduction and application, explanation of evaluation information to the student. Emphasizing the importance of the course in the curriculum and its interaction with other courses. | No pre-preparation | Öğretim Yöntemleri: Anlatım |
| 2 | Tissue acceptance-record-examination and photographing the findings, | Reading a textbook | Öğretim Yöntemleri: Anlatım |
| 3 | What are the tissue tracking and paraffin blocking processes and how they are done. | Reading a textbook | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | How to take a section from the block and stain it with routine pathological dyes. | Reading sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Gösteri |
| 5 | Examination of the prepared preparation under the light microscope | Doing research on the internet, reading textbooks | Öğretim Yöntemleri: Anlatım, Tartışma, Gösterip Yaptırma |
| 6 | Reporting and photographing findings | slide review | Öğretim Yöntemleri: Anlatım, Gösteri |
| 7 | How to accept tissue or sample for cytological examination, recording and observation. | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 8 | Mid-Term Exam | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 9 | Placing the cytological sample on the slide (scrape, smear, touch preparation) and application | Reading sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Preparation of the dye for the cytological preparation and staining of the sample | Doing research on the internet, reading sources | Öğretim Yöntemleri: Anlatım, Gösteri |
| 11 | Points to be considered in the examination of the cytological preparation under the light microscope | Doing homework | Öğretim Yöntemleri: Anlatım, Tartışma |
| 12 | Preparation, application and evaluation of blood cytology | Slide review | Öğretim Yöntemleri: Gösterip Yaptırma |
| 13 | Preparation, application and evaluation of smear (nasal cavity, vaginal, joint etc.). | Reading sources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Preparation, application and evaluation of the imprint cytological preparation | Reading textbooks, doing research on the internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Student presentations and critical giving | Reading a textbook | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams I | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 17 | Term Exams | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 108 | ||
| Total Workload / 25 (h) | 4,32 | ||
| ECTS | 4 ECTS | ||