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Information
| Code | BİO526 |
| Name | Medical Biology Laboratory Applications (Z) |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 0-10 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 10 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Uygulamalı Ders |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. HÜSNÜ ÜMİT LÜLEYAP |
Course Goal / Objective
The purpose of this lecture is to teach students the principles of laboratory practice, cleaning of laboratories, preliminaries for the practises of the lectures Medical Biology and Genetics, which is given to term-1 students and to give an introduction of materials and devices on cytogenetics and molecular-genetics laboratories, material acceptance, conservation of materials, principles of working with materials, and for our routine services, to teach them planning and programming and all the laboratory studies carried out at Medical Biology Department.
Course Content
The content of this lecture is to provide students with the required information about the principles of laboratory practice, cleaning of laboratories, preliminaries for the practises of the lectures Medical Biology and Genetics ,which is given to term-1 students and to give an introduction of materials and devices on cytogenetics and molecular-genetics laboratories, material acceptance for routine services, conservation of materials, principles of working with materials, and to give them a sense of responsibility for the procedures of routine services.
Course Precondition
None
Resources
Prof.Dr. Halil Kasap, Prof.Dr. Mülkiye Kasap, Prof.Dr. Osman Demirhan, Prof.Dr. Davut Alptekin, Prof.Dr. H. Ümit Lüleyap, Prof.Dr. Ayfer Pazarbaşı, Prof.Dr. Ali İrfan Güzel. Medical Biology and Genetics. Nobel Kitabevi, Genişletilmişl 3. Baskı. 2020.
Notes
Lecturer Lecture Notes and Slides
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the principles of laboratory practise. |
| LO02 | Knows how to clean the laboratory. |
| LO03 | Knows the preliminaries for the practises of the lectures Medical Biology and Genetics, which is given to term-1 students. |
| LO04 | Knows the materials and devices on cytogenetics and molecular genetics laboratories. |
| LO05 | Knows material acceptance for routine services, conservation of materials, and studying principles with materials. |
| LO06 | Has a sense of responsibility for the procedures of routine services. |
| LO07 | Should be able to perform blood and tissue culture from routine samples. |
| LO08 | Be able to apply the QF-PCR technique and analyze the results. |
| LO09 | Should be able to isolate DNA from any tissue. |
| LO10 | Should be able to PCR for any gene and walk in gel electrophoresis. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To comprehend the structure and functions of cells and organelles which constitute the basic structure of life | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To recognize the functions of tissue and organ systems | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To examine the structure of genetic material | |
| PLO04 | Bilgi - Kuramsal, Olgusal | To recognize genetic diseases and genes that cause these diseases | |
| PLO05 | Bilgi - Kuramsal, Olgusal | To learn the structure of genes | |
| PLO06 | Bilgi - Kuramsal, Olgusal | To define chromosome and gene mutations | |
| PLO07 | Bilgi - Kuramsal, Olgusal | To classify heredity forms (autosomal dominant, autosomal recessive, gender-dependent dominant and recessive inheritance models) | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To use molecular and cytogenetic analysis | |
| PLO09 | Bilgi - Kuramsal, Olgusal | To be able to explain the numerical and structural anomalies of autosomal and sex chromosomes | 2 |
| PLO10 | Bilgi - Kuramsal, Olgusal | To make DNA isolation from all kinds of tissues | 2 |
| PLO11 | Bilgi - Kuramsal, Olgusal | To prepare PCR | |
| PLO12 | Bilgi - Kuramsal, Olgusal | To make and interpret agarose and polyacrylamide gel electrophoresis | 2 |
| PLO13 | Bilgi - Kuramsal, Olgusal | To measure the obtained DNA on the spectrophotometer | 2 |
| PLO14 | Bilgi - Kuramsal, Olgusal | To apply and interpret QF-PCR technique | |
| PLO15 | Bilgi - Kuramsal, Olgusal | To make cell culture from blood, amnion, chorionic villus biopsy specimens and to obtain chromosome preparation | 5 |
| PLO16 | Bilgi - Kuramsal, Olgusal | To perform karyotype analysis of chromosome preparations and to recognize and interpret all human chromosomes | 5 |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | To interpret a scientific article and to learn how to write a paper | 4 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Principles of laboratory practice and Introduction of the laboratory setting and the devices | Device preparation in laboratory environment | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Types of microscopes and the principles involved in using them | Preparation of different types of microscopes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Using the microscope | Preparation of different types of microscopes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | Techniques of preparation | Preparation of materials such as Lame-Lamellae for the microscope | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Examination of plant and animal cells | Preparation of materials such as Lame-Lamellae for the microscope | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Examination of animal tissues | Preparation of materials such as Lame-Lamellae for the microscope | Öğretim Yöntemleri: Gösteri |
| 7 | Examination of animal limbs | Supplying animal limbs (sheep heart, lung, brain and kidney, etc) | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Exam preparations in the form of questions-answers | Ölçme Yöntemleri: Proje / Tasarım |
| 9 | The animal dissection (mouse and frog) | Supplying a frog and mouse | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Examination of a living sperm and ovum cell | Supplying frog ovum and testicle | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | Examination of giant chromosomes | Supplying a giant chromosome from salivary glands of mosquitos and malpighia tubules and painting them | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 12 | Examination of human chromosomes | Preparation of metaphasis plate obtained from human blood | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 13 | DNA isolation from blood | Supplying the required chemicals and solutions for human blood and DNA isolation | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 14 | DNA isolation from tissue | Supplying the required chemicals and solutions for human tissue and DNA isolation | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 15 | The electrophoresis methods and agarose gel electrophoresis | Supplying the required materials for electrophoresi | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 16 | Term Exams | Exam preparations in the form of questions-answers | Öğretim Yöntemleri: Soru-Cevap, Beyin Fırtınası |
| 17 | Term Exams | Exam preparations in the form of questions-answers | Öğretim Yöntemleri: Soru-Cevap, Beyin Fırtınası |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 9 | 126 |
| Out of Class Study (Preliminary Work, Practice) | 0 | 0 | 0 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 0 | 0 | 0 |
| Final Exam | 1 | 1 | 1 |
| Total Workload (Hour) | 127 | ||
| Total Workload / 25 (h) | 5,08 | ||
| ECTS | 5 ECTS | ||