TÜRKÇE
ENGLISH
Information
Course Goal / Objective
The aim of this course is to comprehensively teach students the basic principles of oxidative stress, mechanisms of cellular damage, its role in the pathogenesis of various diseases, methods of measuring oxidative stress, and antioxidant treatment approaches.
Course Content
Oxidative Stress Fundamentals(Free radicals and reactive oxygen species (ROS), Antioxidant defense systems (enzymatic and non-enzymatic), Definition and mechanisms of oxidative stress, Sources of ROS formation in cells (mitochondria, NADPH oxidase, etc.), Physiological and pathological roles of oxidative stress)-I., Oxidative Stress Fundamentals(Free radicals and reactive oxygen species (ROS), Antioxidant defense systems (enzymatic and non-enzymatic), Definition and mechanisms of oxidative stress, Sources of ROS formation in cells (mitochondria, NADPH oxidase, etc.), Physiological and pathological roles of oxidative stress)-II., Cellular Damage Mechanisms of Oxidative Stress (Lipid peroxidation and membrane damage, Protein oxidation and loss of function, DNA damage and mutations, Carbonyl stress, Changes in cell signaling and gene expression, Apoptosis and necrosis)-I., Cellular Damage Mechanisms of Oxidative Stress (Lipid peroxidation and membrane damage, Protein oxidation and loss of function, DNA damage and mutations, Carbonyl stress, Changes in cell signaling and gene expression, Apoptosis and necrosis)-II., The Role of Oxidative Stress in the Pathogenesis of Diseases (Neurodegenerative Diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis), Cardiovascular Diseases (Atherosclerosis, hypertension, heart failure), Cancer (Carcinogenesis, tumor growth and metastasis), Diabetes and Metabolic Syndrome (Insulin resistance, complications of obesity), Inflammatory Diseases (Rheumatoid arthritis, inflammatory bowel disease), Lung Diseases (Asthma, COPD, lung cancer), Kidney Diseases (Chronic renal failure, glomerulonephritis), Aging (The role of oxidative stress in the aging process))-I., The Role of Oxidative Stress in the Pathogenesis of Diseases (Neurodegenerative Diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis), Cardiovascular Diseases (Atherosclerosis, hypertension, heart failure), Cancer (Carcinogenesis, tumor growth and metastasis), Diabetes and Metabolic Syndrome (Insulin resistance, complications of obesity), Inflammatory Diseases (Rheumatoid arthritis, inflammatory bowel disease), Lung Diseases (Asthma, COPD, lung cancer), Kidney Diseases (Chronic renal failure, glomerulonephritis), Aging (The role of oxidative stress in the aging process))-II., The Role of Oxidative Stress in the Pathogenesis of Diseases (Neurodegenerative Diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis), Cardiovascular Diseases (Atherosclerosis, hypertension, heart failure), Cancer (Carcinogenesis, tumor growth and metastasis), Diabetes and Metabolic Syndrome (Insulin resistance, complications of obesity), Inflammatory Diseases (Rheumatoid arthritis, inflammatory bowel disease), Lung Diseases (Asthma, COPD, lung cancer), Kidney Diseases (Chronic renal failure, glomerulonephritis), Aging (The role of oxidative stress in the aging process))-III., Oxidative Stress Measurement Methods (Direct Measurements (ROS measurement (e.g., DCF-DA, hydroxyl radical measurement), Lipid peroxidation measurement (e.g., MDA, 4-HNE), Protein oxidation measurement (e.g., protein carbonyl, thiol measurement), DNA damage measurement (e.g., 8-OHdG), Indirect Measurements (Antioxidant capacity measurement (e.g., FRAP, ORAC), Glutathione/Glutathione disulfide (GSH/GSSG) ratio, Enzymatic antioxidant activity measurement (e.g., SOD, CAT, GPx))-I., Oxidative Stress Measurement Methods (Direct Measurements (ROS measurement (e.g., DCF-DA, hydroxyl radical measurement), Lipid peroxidation measurement (e.g., MDA, 4-HNE), Protein oxidation measurement (e.g., protein carbonyl, thiol measurement), DNA damage measurement (e.g., 8-OHdG), Indirect Measurements (Antioxidant capacity measurement (e.g., FRAP, ORAC), Glutathione/Glutathione disulfide (GSH/GSSG) ratio, Enzymatic antioxidant activity measurement (e.g., SOD, CAT, GPx))-II., Oxidative Stress Measurement Methods (Direct Measurements (ROS measurement (e.g., DCF-DA, hydroxyl radical measurement), Lipid peroxidation measurement (e.g., MDA, 4-HNE), Protein oxidation measurement (e.g., protein carbonyl, thiol measurement), DNA damage measurement (e.g., 8-OHdG), Indirect Measurements (Antioxidant capacity measurement (e.g., FRAP, ORAC), Glutathione/Glutathione disulfide (GSH/GSSG) ratio, Enzymatic antioxidant activity measurement (e.g., SOD, CAT, GPx))-III., Antioxidant Treatment Approaches (Dietary Antioxidant Intake (Vitamins (Vitamin C, Vitamin E), Polyphenols (flavonoids, resveratrol), Carotenoids (lycopene, β-carotene), Minerals (selenium, zinc), Antioxidant Supplements (Vitamin supplements, Mineral supplements, Herbal supplements), Synthetic Antioxidants (N-acetylcysteine (NAC), α-lipoic acid), New Antioxidant Strategies (Targeted antioxidants, Mitochondrial antioxidants, Gene therapy))-I., Antioxidant Treatment Approaches (Dietary Antioxidant Intake (Vitamins (Vitamin C, Vitamin E), Polyphenols (flavonoids, resveratrol), Carotenoids (lycopene, β-carotene), Minerals (selenium, zinc), Antioxidant Supplements (Vitamin supplements, Mineral supplements, Herbal supplements), Synthetic Antioxidants (N-acetylcysteine (NAC), α-lipoic acid), New Antioxidant Strategies (Targeted antioxidants, Mitochondrial antioxidants, Gene therapy))-II., Current Topics in Oxidative Stress Research (The role of oxidative stress in signaling pathways, The relationship between oxidative stress and inflammation, Oxidative stress and epigenetic changes, The place of oxidative stress in personalized medicine, New approaches for the prevention and treatment of oxidative stress)-I., Current Topics in Oxidative Stress Research (The role of oxidative stress in signaling pathways, The relationship between oxidative stress and inflammation, (The role of oxidative stress, the relationship between oxidative stress and inflammation, oxidative stress and epigenetic changes, the place of oxidative stress in personalized medicine, new approaches for the prevention and treatment of oxidative stress)-II.
Course Precondition
Not required.
Resources
Kıran, T. R., Otlu, O., & Karabulut, A. B. (2023). Oxidative stress and antioxidants in health and disease. Journal of Laboratory Medicine, 47(1), 1-11.
Notes
Current scientific articles and journals (e.g., Free Radical Biology and Medicine, Antioxidants & Redox Signaling), Internet sources (e.g., PubMed, Google Scholar)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explains the basics of oxidative stress and the cellular damage mechanisms of oxidative stress. |
| LO02 | Describes the role of oxidative stress in the pathogenesis of diseases. |
| LO03 | Counts the methods of measuring oxidative stress. |
| LO04 | Describes antioxidant treatment approaches and current issues in oxidative stress research. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops and deepens the current and advanced level knowledge of translational medicine via unique thoughts or researches at a level of proficiency, find out original definitions that will bring innovation in the field of translational medicine. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Conceives the interdisciplinary interactions related to translational medicine; analyzes synthesizes and evaluates original and new thoughts. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Explains the usage of tool, devices and instruments requiered for knowledge and technologies about translational medicine and its related disciplines. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Defines frequently used statistical methods in translational medicine and related disciplines, uses statistical softwares effectively. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Uses both theoretical and practical knowledge at an advanced level in the studies related to translational medicine. | |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Develops a new thoughts, method and designment/application which brings innovation in translational medicine or implements a known thoughts, method and designment/application in different fields, investigates, comprehends, designs, adapts, implements an original topic. | |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Writes the report of his/her research which he/she participated, | 4 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Makes necessary investigations by using tool, devices and instrument required for knowledge and technologies about translational medicine and related disciplines at an advanced level, develops a new and creative solution (device, method, treatment, drug) for the problems. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Uses statistical software in the field of Translational Medicine effectively, chooses statistical methods correctly, calculates and interprets correctly. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Maintains the organization of Translational Medicine laboratories and develops solutions in case of encountering unforeseen complex situations during laboratory working hours. | 3 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Conducts scientific studies in translational medicine and related fields independently or as a team member. | 3 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Writes the report of his/her research in which he/she participated, publishes in a national or international reputed journal (indexing in SCI, SCI-Expanded, SSCI, or AHCI), and presents it at scientific meetings. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Follows evidence-based practices and conducts research on professional practices that will create evidence in their field. | 3 |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Applies the principles of advanced professional development and lifelong learning in the field of Translational Medicine. | |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicate current developments and studies within the field to both professional and non-professional groups systematically using written, oral and visual techniques by supporting with quantitative and qualitative data. | 4 |
| PLO16 | Bilgi - Kuramsal, Olgusal | Learns how to teach a concept, subject, phenomenon or case related to Translational Medicine. | |
| PLO17 | Belirsiz | Communicate and discuss orally, in written and visually with peers by using a foreign language at least at a level of European Language Portfolio B2 General Level. | |
| PLO18 | Yetkinlikler - Alana Özgü Yetkinlik | Audit the data gathering, interpretation, implementation and announcement stages by taking into consideration the cultural, scientific, and ethic values and uses these issues for social strategy, implementation plans and frame of quality processes. | |
| PLO19 | Yetkinlikler - Alana Özgü Yetkinlik | Knows the importance of ethical principles and ethical committees for the individual and society, acts ethically. | |
| PLO20 | Yetkinlikler - Alana Özgü Yetkinlik | Increases the awareness of society about translational medicine. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Oxidative Stress Fundamentals(Free radicals and reactive oxygen species (ROS), Antioxidant defense systems (enzymatic and non-enzymatic), Definition and mechanisms of oxidative stress, Sources of ROS formation in cells (mitochondria, NADPH oxidase, etc.), Physiological and pathological roles of oxidative stress)-I. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Oxidative Stress Fundamentals(Free radicals and reactive oxygen species (ROS), Antioxidant defense systems (enzymatic and non-enzymatic), Definition and mechanisms of oxidative stress, Sources of ROS formation in cells (mitochondria, NADPH oxidase, etc.), Physiological and pathological roles of oxidative stress)-II. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Cellular Damage Mechanisms of Oxidative Stress (Lipid peroxidation and membrane damage, Protein oxidation and loss of function, DNA damage and mutations, Carbonyl stress, Changes in cell signaling and gene expression, Apoptosis and necrosis)-I. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | Cellular Damage Mechanisms of Oxidative Stress (Lipid peroxidation and membrane damage, Protein oxidation and loss of function, DNA damage and mutations, Carbonyl stress, Changes in cell signaling and gene expression, Apoptosis and necrosis)-II. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | The Role of Oxidative Stress in the Pathogenesis of Diseases (Neurodegenerative Diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis), Cardiovascular Diseases (Atherosclerosis, hypertension, heart failure), Cancer (Carcinogenesis, tumor growth and metastasis), Diabetes and Metabolic Syndrome (Insulin resistance, obesity complications), Inflammatory Diseases (Rheumatoid arthritis, inflammatory bowel disease), Lung Diseases (Asthma, COPD, lung cancer), Kidney Diseases (Chronic renal failure, glomerulonephritis), Aging (The role of oxidative stress in the aging process))-I. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | The Role of Oxidative Stress in the Pathogenesis of Diseases (Neurodegenerative Diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis), Cardiovascular Diseases (Atherosclerosis, hypertension, heart failure), Cancer (Carcinogenesis, tumor growth and metastasis), Diabetes and Metabolic Syndrome (Insulin resistance, obesity complications), Inflammatory Diseases (Rheumatoid arthritis, inflammatory bowel disease), Lung Diseases (Asthma, COPD, lung cancer), Kidney Diseases (Chronic renal failure, glomerulonephritis), Aging (The role of oxidative stress in the aging process))-II. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 7 | The Role of Oxidative Stress in the Pathogenesis of Diseases (Neurodegenerative Diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis), Cardiovascular Diseases (Atherosclerosis, hypertension, heart failure), Cancer (Carcinogenesis, tumor growth and metastasis), Diabetes and Metabolic Syndrome (Insulin resistance, obesity complications), Inflammatory Diseases (Rheumatoid arthritis, inflammatory bowel disease), Lung Diseases (Asthma, COPD, lung cancer), Kidney Diseases (Chronic renal failure, glomerulonephritis), Aging (The role of oxidative stress in the aging process))-III. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Proje / Tasarım |
|
| 9 | Oxidative Stress Measurement Methods (Direct Measurements (ROS measurement (e.g., DCF-DA, hydroxyl radical measurement), Lipid peroxidation measurement (e.g., MDA, 4-HNE), Protein oxidation measurement (e.g., protein carbonyl, thiol measurement), DNA damage measurement (e.g., 8-OHdG), Indirect Measurements (Antioxidant capacity measurement (e.g., FRAP, ORAC), Glutathione/Glutathione disulfide (GSH/GSSG) ratio, Enzymatic antioxidant activity measurement (e.g., SOD, CAT, GPx))-I. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Oxidative Stress Measurement Methods (Direct Measurements (ROS measurement (e.g., DCF-DA, hydroxyl radical measurement), Lipid peroxidation measurement (e.g., MDA, 4-HNE), Protein oxidation measurement (e.g., protein carbonyl, thiol measurement), DNA damage measurement (e.g., 8-OHdG), Indirect Measurements (Antioxidant capacity measurement (e.g., FRAP, ORAC), Glutathione/Glutathione disulfide (GSH/GSSG) ratio, Enzymatic antioxidant activity measurement (e.g., SOD, CAT, GPx))-II. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Oxidative Stress Measurement Methods (Direct Measurements (ROS measurement (e.g., DCF-DA, hydroxyl radical measurement), Lipid peroxidation measurement (e.g., MDA, 4-HNE), Protein oxidation measurement (e.g., protein carbonyl, thiol measurement), DNA damage measurement (e.g., 8-OHdG), Indirect Measurements (Antioxidant capacity measurement (e.g., FRAP, ORAC), Glutathione/Glutathione disulfide (GSH/GSSG) ratio, Enzymatic antioxidant activity measurement (e.g., SOD, CAT, GPx))-III. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | Antioxidant Treatment Approaches (Dietary Antioxidant Intake (Vitamins (vitamin C, vitamin E), Polyphenols (flavonoids, resveratrol), Carotenoids (lycopene, β-carotene), Minerals (selenium, zinc), Antioxidant Supplements (Vitamin supplements, Mineral supplements, Herbal supplements), Synthetic Antioxidants (N-acetylcysteine (NAC), α-lipoic acid), New Antioxidant Strategies (Targeted antioxidants, Mitochondrial antioxidants, Gene therapy))-I. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Antioxidant Treatment Approaches (Dietary Antioxidant Intake (Vitamins (vitamin C, vitamin E), Polyphenols (flavonoids, resveratrol), Carotenoids (lycopene, β-carotene), Minerals (selenium, zinc), Antioxidant Supplements (Vitamin supplements, Mineral supplements, Herbal supplements), Synthetic Antioxidants (N-acetylcysteine (NAC), α-lipoic acid), New Antioxidant Strategies (Targeted antioxidants, Mitochondrial antioxidants, Gene therapy))-II. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Current Topics in Oxidative Stress Research (Role of oxidative stress in signaling pathways, Relationship between oxidative stress and inflammation, Oxidative stress and epigenetic changes, Place of oxidative stress in personalized medicine, New approaches for prevention and treatment of oxidative stress)-I. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Current Topics in Oxidative Stress Research (Role of oxidative stress in signaling pathways, Relationship between oxidative stress and inflammation, Oxidative stress and epigenetic changes, Place of oxidative stress in personalized medicine, New approaches for prevention and treatment of oxidative stress)-II. | books and scientific databases | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Proje / Tasarım |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Proje / Tasarım |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||