TÜRKÇE
ENGLISH
Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| TRANSLATIONAL MEDICINE (MASTER) (WITH THESIS) (ENGLISH) (INTERDISCIPLINARY) | |
| Code | Trans555 |
| Name | Molecular Biology Laboratory Techniques I |
| Term | 2025-2026 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 1-4 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | İngilizce |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
Prof. Dr. MEHMET BERTAN YILMAZ
(Güz)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
Understanding Basic Molecular Biology Laboratory Techniques
Course Content
Analysis of Molecular Biology Laboratory Techniques
Course Precondition
None
Resources
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri
Notes
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Comprehends the methods used in basic molecular biology |
| LO02 | Knows which molecular biological technique to choose in which study |
| LO03 | Has knowledge of the basic concepts of molecular biology |
| LO04 | Knows how to apply molecular biological methods |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops and deepens knowledge in the field of translational medicine to the proficiency level based on bachelor-level qualifications. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Conceives the interdisciplinary interaction in which the field is related to translational medicine | 4 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Knows the information technologies, technical equipment, and field-specific devices and instruments at the level required by the field of Translational Medicine. | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows statistics as a tool that is constantly used in studies in the field of Translational Medicine, and uses relevant software effectively. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Uses of theoretical and practical knowledge within the field at a proficiency level. | |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Interprets the knowledge about the field by integrating the information gathered from different disciplines and formulates new knowledge. | 5 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Makes necessary investigations by using tools, devices, and instruments required for knowledge and technologies about translational medicine and related disciplines at an advanced level; develops a new and creative solution (device, method, treatment, drug) for the problems. | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Solves the problem faced related to the field by using research methods. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Plans and conducts an experimental study. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes responsibility and develops new strategic solutions as a team member in order to solve unexpected complex problems faced within the applications in the field, evaluates the results, and applies when necessary. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Conducts scientific studies in translational medicine and related fields independently or as a team member. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Publishes a scientific paper in a national journal or presents it at a scientific meeting. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Evaluates knowledge and skills acquired at proficiency level in the field with a critical approach and direct the learning. | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Applies the principles of advanced professional development and lifelong learning in the field of Translational Medicine. | |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicates current developments and studies within the field to both professional and non-professional groups systematically using written, oral and visual techniques by supporting quantitative and qualitative data. | |
| PLO16 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicates and discusses orally, in writing, and visually with peers by using a foreign language at least at a level of European Language Portfolio B2 General Level. | |
| PLO17 | Yetkinlikler - Alana Özgü Yetkinlik | Develops strategy, policy, and implementation plans on the issues related to the field and assess the findings within the frame of quality processes and ethics. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | The importance of nucleic acids in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Methods used in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | DNA isolation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | RNA isolation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Protein isolation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Protein assay | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Investigation of molecular biological methods in the literature | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Ödev |
|
| 9 | Eliza method and application areas | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | PCR variants | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Real time PCR | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Agarose gel electrophoresis and interpretation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Western blot method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Literature search of current molecular biological methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Literature search of current molecular biological methods II | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 8 | 16 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 7 | 7 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||