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Information
| Unit | |
| Code | SD0842 |
| Name | Spectroscopic Techniques |
| Term | 2026-2027 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Üniversite Dersi |
| Label | UCC University Common Course |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Öğr. Gör. UĞUR ÇAĞLAYAN |
| Course Instructor |
Öğr. Gör. UĞUR ÇAĞLAYAN
(Güz)
(A Group)
(Ins. in Charge)
Öğr. Gör. UĞUR ÇAĞLAYAN (Güz) (B Group) (Ins. in Charge) |
Course Goal / Objective
To introduce the principles of spectroscopic analysis, atomic and molecular spectroscopy, instrument components, method development, qualitative and quantitative analysis, and practical applications.
Course Content
This course covers the fundamental principles of electromagnetic radiation-matter interactions, as well as the theoretical foundations and instrumentation of major atomic (AAS, ICP, etc.) and molecular (UV-Vis, FT-IR, NMR, MS, etc.) spectroscopic techniques. Throughout the course, students are provided with practical and theoretical knowledge on sample preparation, spectrum interpretation, qualitative and quantitative analysis, selecting the appropriate spectroscopic technique for analytical problems, and method development.
Course Precondition
Resources
1) Explains the basic principles of spectroscopy. 2) Classifies atomic and molecular spectroscopy techniques. 3) Interprets the working principles of AAS, ICP-OES, and ICP-MS techniques. 4) Explains the application areas of UV-Vis, FTIR, and Raman spectroscopies. 5) Compares the basic characteristics of XRF, XPS, NMR, and EPR techniques.
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explains the regions of the electromagnetic spectrum and the fundamental theoretical principles of light–matter interaction. |
| LO02 | Defines fundamental optical phenomena such as absorption, emission, and scattering, as well as transitions between atomic/molecular energy levels |
| LO03 | Classifies spectroscopic analysis techniques into atomic and molecular methods and differentiates between them. |
| LO04 | Explains the working principles of Atomic Absorption Spectroscopy (AAS), atomization systems, and matrix interferences. |
| LO05 | Compares the fundamental theoretical principles of Atomic Emission Spectroscopy (AES), ICP-OES, and ICP-MS techniques. |
| LO06 | Describes the instrumental components of ICP systems and applies sample preparation and method development steps for elemental analysis. |
| LO07 | Evaluates the fundamental mechanisms of UV-Vis spectroscopy and the concepts of chromophores/auxochromes in practical applications. |
| LO08 | Compares the principles of Raman scattering with FTIR spectroscopy and selects the appropriate method based on application areas. |
| LO09 | Defines the basic principles of XRF and XPS surface/elemental analysis techniques and their application areas in material characterization. |
| LO10 | Explains the working mechanisms of NMR, EPR, and fluorescence spectroscopy techniques and their roles in structure elucidation. |
| LO11 | Evaluates spectroscopic analytical data to construct calibration curves and performs accuracy and uncertainty calculations. |
| LO12 | Identifies the most suitable spectroscopic technique for analytical problems in materials science, environmental science, food science, geology, and biomedical fields. |
| LO13 | Explains molecular vibration theory to acquire skills in sample preparation and functional group spectrum interpretation using FTIR spectroscopy. |
| LO14 | Demonstrates practical skills in sample preparation, instrument configuration, calibration, and quantitative result reporting using AAS and ICP instruments. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to spectroscopy and electromagnetic radiation | Reading the introductory chapter of the textbook and reviewing the electromagnetic spectrum regions. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Light–matter interaction (absorption, emission etc.) | Review of basic physics/chemistry concepts including relations between photon energy, wavelength, frequency, and energy levels | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Classification of spectroscopic techniques (atomic and molecular spectroscopy) | Reviewing lecture notes regarding the differences between atomic and molecular structures. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | Atomic absorption spectroscopy (AAS): principles, atomization systems, interferences. | Reading basic principles of AAS and researching flame and graphite furnace atomization systems. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | Basic principles of atomic emission spectroscopy (AES), ICP-OES, and ICP-MS. | Studying plasma technology and the principles of plasma-based emission/mass spectrometry. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | ICP-OES and ICP-MS instrument components, sample preparation, and method development. | Reviewing laboratory safety rules, reading on acid digestion techniques and matrix effect concepts. | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Deney / Laboratuvar |
| 7 | Molecular UV-Vis spectroscopy and its applications. | Reading working principles of UV-Vis spectrophotometers and reviewing Beer-Lambert's law. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Alıştırma ve Uygulama, Deney / Laboratuvar |
| 8 | Mid-Term Exam | General review of theoretical and practical course materials from weeks 1 to 7. | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | FTIR spectroscopy: vibration theory, sample preparation, and interpretation. | Studying molecular vibration types (stretching, bending, etc.) and FTIR sample preparation methods (KBr pellet, etc.). | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Deney / Laboratuvar |
| 10 | Raman spectroscopy: basic principles, Raman/FTIR comparison, and applications. | Researching elastic and inelastic scattering (Rayleigh, Raman) and reading the differences between FTIR and Raman. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Deney / Laboratuvar |
| 11 | Introduction to XRF and XPS techniques and their application areas. | Reviewing fundamental concepts of X-ray interactions with matter and surface analysis techniques. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | An overview of NMR, EPR, and fluorescence spectroscopy. | Preliminary reading on nuclear spin, coupling, and fluorescence phenomena. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Evaluation of spectroscopic data, calibration, accuracy, and uncertainty. | Studying calibration curve construction, standard deviation, accuracy, precision, and uncertainty calculations. | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar, Alıştırma ve Uygulama |
| 14 | Examples of spectroscopy-suitable applications from materials science, environmental science, food science, geology, and biomedical fields. | Examining sample literature articles or case studies involving real sample analyses. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Deney / Laboratuvar, Alıştırma ve Uygulama |
| 15 | Application: Sample preparation, instrument familiarization, analysis, and evaluation of results in AAS and ICP-OES/ICP-MS instruments. | reviewing sample solution preparation and analysis procedure steps. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Deney / Laboratuvar, Alıştırma ve Uygulama |
| 16 | Term Exams | Comprehensive review of all semester course content and laboratory applications. | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Final exam preparation and solving sample analytical problems. | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 80 | ||
| Total Workload / 25 (h) | 3,20 | ||
| ECTS | 3 ECTS | ||